Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro

Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host...

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Main Authors: Marie Wiatr, Simon Staubach, Ricardo Figueiredo, Carolin Stump-Guthier, Hiroshi Ishikawa, Christian Schwerk, Horst Schroten, Franz-Georg Hanisch, Henriette Rudolph, Tobias Tenenbaum
Format: Article
Language:English
Published: MDPI AG 2020-12-01
Series:Microorganisms
Subjects:
Online Access:https://www.mdpi.com/2076-2607/8/12/1958
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author Marie Wiatr
Simon Staubach
Ricardo Figueiredo
Carolin Stump-Guthier
Hiroshi Ishikawa
Christian Schwerk
Horst Schroten
Franz-Georg Hanisch
Henriette Rudolph
Tobias Tenenbaum
author_facet Marie Wiatr
Simon Staubach
Ricardo Figueiredo
Carolin Stump-Guthier
Hiroshi Ishikawa
Christian Schwerk
Horst Schroten
Franz-Georg Hanisch
Henriette Rudolph
Tobias Tenenbaum
author_sort Marie Wiatr
collection DOAJ
description Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.
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spelling doaj.art-0291d8fc538846f48a1b5948bc7e18b12023-11-21T00:10:57ZengMDPI AGMicroorganisms2076-26072020-12-01812195810.3390/microorganisms8121958Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In VitroMarie Wiatr0Simon Staubach1Ricardo Figueiredo2Carolin Stump-Guthier3Hiroshi Ishikawa4Christian Schwerk5Horst Schroten6Franz-Georg Hanisch7Henriette Rudolph8Tobias Tenenbaum9Pediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyInstitute for Transfusion Medicine, University Hospital of Essen, University of Druisburg-Essen, 45157 Essen, GermanyGenXPro GmbH, 60438 Frankfurt am Main, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyLaboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, Tennodai, Tsukuba, Ibaraki 305-8575, JapanPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyInstitute of Biochemistry II, Medical Faculty, University of Cologne, 50931 Cologne, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyEchovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.https://www.mdpi.com/2076-2607/8/12/1958enterovirusEchovirus-30lipid raftblood-cerebrospinal fluid barrierviral infectionHIBCPP cells
spellingShingle Marie Wiatr
Simon Staubach
Ricardo Figueiredo
Carolin Stump-Guthier
Hiroshi Ishikawa
Christian Schwerk
Horst Schroten
Franz-Georg Hanisch
Henriette Rudolph
Tobias Tenenbaum
Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
Microorganisms
enterovirus
Echovirus-30
lipid raft
blood-cerebrospinal fluid barrier
viral infection
HIBCPP cells
title Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_full Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_fullStr Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_full_unstemmed Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_short Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
title_sort echovirus 30 infection alters host proteins in lipid rafts at the cerebrospinal fluid barrier in vitro
topic enterovirus
Echovirus-30
lipid raft
blood-cerebrospinal fluid barrier
viral infection
HIBCPP cells
url https://www.mdpi.com/2076-2607/8/12/1958
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