Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro
Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host...
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MDPI AG
2020-12-01
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Online Access: | https://www.mdpi.com/2076-2607/8/12/1958 |
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author | Marie Wiatr Simon Staubach Ricardo Figueiredo Carolin Stump-Guthier Hiroshi Ishikawa Christian Schwerk Horst Schroten Franz-Georg Hanisch Henriette Rudolph Tobias Tenenbaum |
author_facet | Marie Wiatr Simon Staubach Ricardo Figueiredo Carolin Stump-Guthier Hiroshi Ishikawa Christian Schwerk Horst Schroten Franz-Georg Hanisch Henriette Rudolph Tobias Tenenbaum |
author_sort | Marie Wiatr |
collection | DOAJ |
description | Echovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration. |
first_indexed | 2024-03-10T14:10:21Z |
format | Article |
id | doaj.art-0291d8fc538846f48a1b5948bc7e18b1 |
institution | Directory Open Access Journal |
issn | 2076-2607 |
language | English |
last_indexed | 2024-03-10T14:10:21Z |
publishDate | 2020-12-01 |
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series | Microorganisms |
spelling | doaj.art-0291d8fc538846f48a1b5948bc7e18b12023-11-21T00:10:57ZengMDPI AGMicroorganisms2076-26072020-12-01812195810.3390/microorganisms8121958Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In VitroMarie Wiatr0Simon Staubach1Ricardo Figueiredo2Carolin Stump-Guthier3Hiroshi Ishikawa4Christian Schwerk5Horst Schroten6Franz-Georg Hanisch7Henriette Rudolph8Tobias Tenenbaum9Pediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyInstitute for Transfusion Medicine, University Hospital of Essen, University of Druisburg-Essen, 45157 Essen, GermanyGenXPro GmbH, 60438 Frankfurt am Main, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyLaboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine, University of Tsukuba, Tennodai, Tsukuba, Ibaraki 305-8575, JapanPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyInstitute of Biochemistry II, Medical Faculty, University of Cologne, 50931 Cologne, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyPediatric Infectious Diseases, University Children’s Hospital Mannheim, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, GermanyEchovirus-30 (E-30) is a non-polio enterovirus responsible for meningitis outbreaks in children worldwide. To gain access to the central nervous system (CNS), E-30 first has to cross the blood-brain barrier (BBB) or the blood-cerebrospinal fluid barrier (BCSFB). E-30 may use lipid rafts of the host cells to interact with and to invade the BCSFB. To study enteroviral infection of the BCSFB, an established in vitro model based on human immortalized brain choroid plexus papilloma (HIBCPP) cells has been used. Here, we investigated the impact of E-30 infection on the protein content of the lipid rafts at the BCSFB in vitro. Mass spectrometry analysis following E-30 infection versus uninfected conditions revealed differential abundancy in proteins implicated in cellular adhesion, cytoskeleton remodeling, and endocytosis/vesicle budding. Further, we evaluated the blocking of endocytosis via clathrin/dynamin blocking and its consequences for E-30 induced barrier disruption. Interestingly, blocking of endocytosis had no impact on the capacity of E-30 to induce loss of barrier properties in HIBCPP cells. Altogether, these data highlight the impact of E-30 on HIBCPP cells microdomain as an important factor for host cell alteration.https://www.mdpi.com/2076-2607/8/12/1958enterovirusEchovirus-30lipid raftblood-cerebrospinal fluid barrierviral infectionHIBCPP cells |
spellingShingle | Marie Wiatr Simon Staubach Ricardo Figueiredo Carolin Stump-Guthier Hiroshi Ishikawa Christian Schwerk Horst Schroten Franz-Georg Hanisch Henriette Rudolph Tobias Tenenbaum Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro Microorganisms enterovirus Echovirus-30 lipid raft blood-cerebrospinal fluid barrier viral infection HIBCPP cells |
title | Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro |
title_full | Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro |
title_fullStr | Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro |
title_full_unstemmed | Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro |
title_short | Echovirus-30 Infection Alters Host Proteins in Lipid Rafts at the Cerebrospinal Fluid Barrier In Vitro |
title_sort | echovirus 30 infection alters host proteins in lipid rafts at the cerebrospinal fluid barrier in vitro |
topic | enterovirus Echovirus-30 lipid raft blood-cerebrospinal fluid barrier viral infection HIBCPP cells |
url | https://www.mdpi.com/2076-2607/8/12/1958 |
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