DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation
Abstract Polychlorinated biphenyl (PCB)-contaminated soils represent a major treat for ecosystems health. Plant biostimulation of autochthonous microbial PCB degraders is a way to restore polluted sites where traditional remediation techniques are not sustainable, though its success requires the und...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23728-2 |
| _version_ | 1811230402490138624 |
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| author | Lorenzo Vergani Francesca Mapelli Magdalena Folkmanova Jakub Papik Jan Jansa Ondrej Uhlik Sara Borin |
| author_facet | Lorenzo Vergani Francesca Mapelli Magdalena Folkmanova Jakub Papik Jan Jansa Ondrej Uhlik Sara Borin |
| author_sort | Lorenzo Vergani |
| collection | DOAJ |
| description | Abstract Polychlorinated biphenyl (PCB)-contaminated soils represent a major treat for ecosystems health. Plant biostimulation of autochthonous microbial PCB degraders is a way to restore polluted sites where traditional remediation techniques are not sustainable, though its success requires the understanding of site-specific plant–microbe interactions. In an historical PCB contaminated soil, we applied DNA stable isotope probing (SIP) using 13C-labeled 4-chlorobiphenyl (4-CB) and 16S rRNA MiSeq amplicon sequencing to determine how the structure of total and PCB-degrading bacterial populations were affected by different treatments: biostimulation with Phalaris arundinacea subjected (PhalRed) or not (Phal) to a redox cycle and the non-planted controls (Bulk and BulkRed). Phal soils hosted the most diverse community and plant biostimulation induced an enrichment of Actinobacteria. Mineralization of 4-CB in SIP microcosms varied between 10% in Bulk and 39% in PhalRed soil. The most abundant taxa deriving carbon from PCB were Betaproteobacteria and Actinobacteria. Comamonadaceae was the family most represented in Phal soils, Rhodocyclaceae and Nocardiaceae in non-planted soils. Planted soils subjected to redox cycle enriched PCB degraders affiliated to Pseudonocardiaceae, Micromonosporaceae and Nocardioidaceae. Overall, we demonstrated different responses of soil bacterial taxa to specific rhizoremediation treatments and we provided new insights into the populations active in PCB biodegradation. |
| first_indexed | 2024-04-12T10:28:50Z |
| format | Article |
| id | doaj.art-029a3f85e7774042a9963223eca8a2e3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-12T10:28:50Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-029a3f85e7774042a9963223eca8a2e32022-12-22T03:36:54ZengNature PortfolioScientific Reports2045-23222022-11-0112111010.1038/s41598-022-23728-2DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradationLorenzo Vergani0Francesca Mapelli1Magdalena Folkmanova2Jakub Papik3Jan Jansa4Ondrej Uhlik5Sara Borin6Department of Food, Environmental and Nutritional Sciences, University of MilanDepartment of Food, Environmental and Nutritional Sciences, University of MilanDepartement of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueDepartement of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueLaboratory of Fungal Biology, Institute of Microbiology, Czech Academy of SciencesDepartement of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, University of Chemistry and Technology PragueDepartment of Food, Environmental and Nutritional Sciences, University of MilanAbstract Polychlorinated biphenyl (PCB)-contaminated soils represent a major treat for ecosystems health. Plant biostimulation of autochthonous microbial PCB degraders is a way to restore polluted sites where traditional remediation techniques are not sustainable, though its success requires the understanding of site-specific plant–microbe interactions. In an historical PCB contaminated soil, we applied DNA stable isotope probing (SIP) using 13C-labeled 4-chlorobiphenyl (4-CB) and 16S rRNA MiSeq amplicon sequencing to determine how the structure of total and PCB-degrading bacterial populations were affected by different treatments: biostimulation with Phalaris arundinacea subjected (PhalRed) or not (Phal) to a redox cycle and the non-planted controls (Bulk and BulkRed). Phal soils hosted the most diverse community and plant biostimulation induced an enrichment of Actinobacteria. Mineralization of 4-CB in SIP microcosms varied between 10% in Bulk and 39% in PhalRed soil. The most abundant taxa deriving carbon from PCB were Betaproteobacteria and Actinobacteria. Comamonadaceae was the family most represented in Phal soils, Rhodocyclaceae and Nocardiaceae in non-planted soils. Planted soils subjected to redox cycle enriched PCB degraders affiliated to Pseudonocardiaceae, Micromonosporaceae and Nocardioidaceae. Overall, we demonstrated different responses of soil bacterial taxa to specific rhizoremediation treatments and we provided new insights into the populations active in PCB biodegradation.https://doi.org/10.1038/s41598-022-23728-2 |
| spellingShingle | Lorenzo Vergani Francesca Mapelli Magdalena Folkmanova Jakub Papik Jan Jansa Ondrej Uhlik Sara Borin DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation Scientific Reports |
| title | DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation |
| title_full | DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation |
| title_fullStr | DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation |
| title_full_unstemmed | DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation |
| title_short | DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation |
| title_sort | dna stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in pcb degradation |
| url | https://doi.org/10.1038/s41598-022-23728-2 |
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