CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS
Background Renal failure is a frequent and serious complication in patients with decompensated cirrhosis. Objectives We aimed to evaluate the renal oxidative stress, cell damage and impaired cell function in animal model of cirrhosis. Methods Secondary biliary cirrhosis was induced in rats by ligati...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE)
2015-03-01
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| Series: | Arquivos de Gastroenterologia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032015000100014&lng=en&tlng=en |
| _version_ | 1818570463048105984 |
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| author | Keli Cristina Simões da SILVEIRA Cassiana Macagnan VIAU Josiane Raskopf COLARES Jenifer SAFFI Norma Possa MARRONI Marilene PORAWSKI |
| author_facet | Keli Cristina Simões da SILVEIRA Cassiana Macagnan VIAU Josiane Raskopf COLARES Jenifer SAFFI Norma Possa MARRONI Marilene PORAWSKI |
| author_sort | Keli Cristina Simões da SILVEIRA |
| collection | DOAJ |
| description | Background Renal failure is a frequent and serious complication in patients with decompensated cirrhosis. Objectives We aimed to evaluate the renal oxidative stress, cell damage and impaired cell function in animal model of cirrhosis. Methods Secondary biliary cirrhosis was induced in rats by ligation of the common bile duct. We measured TBARS, ROS and mitochondrial membrane potential in kidney as markers of oxidative stress, and activities of the antioxidant enzymes. Relative cell viability was determined by trypan blue dye-exclusion assay. Annexin V-PE was used with a vital dye, 7-AAD, to distinguish apoptotic from necrotic cells and comet assay was used for determined DNA integrity in single cells. Results In bile duct ligation animals there was significant increase in the kidney lipoperoxidation and an increase of the level of intracellular ROS. There was too an increase in the activity of all antioxidant enzymes evaluated in the kidney. The percentage viability was above 90% in the control group and in bile duct ligation was 64.66% and the dominant cell death type was apoptosis. DNA damage was observed in the bile duct ligation. There was a decreased in the mitochondrial membrane potential from 71.40% ± 6.35% to 34.48% ± 11.40% in bile duct ligation. Conclusions These results indicate that intracellular increase of ROS cause damage in the DNA and apoptosis getting worse the renal function in cirrhosis. |
| first_indexed | 2024-12-14T13:41:42Z |
| format | Article |
| id | doaj.art-029b0340ab5b417eb5a6cb09b3a73ac9 |
| institution | Directory Open Access Journal |
| issn | 1678-4219 |
| language | English |
| last_indexed | 2024-12-14T13:41:42Z |
| publishDate | 2015-03-01 |
| publisher | Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE) |
| record_format | Article |
| series | Arquivos de Gastroenterologia |
| spelling | doaj.art-029b0340ab5b417eb5a6cb09b3a73ac92022-12-21T22:59:26ZengInstituto Brasileiro de Estudos e Pesquisas de Gastroenterologia (IBEPEGE)Arquivos de Gastroenterologia1678-42192015-03-01521657110.1590/S0004-28032015000100014S0004-28032015000100014CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESSKeli Cristina Simões da SILVEIRACassiana Macagnan VIAUJosiane Raskopf COLARESJenifer SAFFINorma Possa MARRONIMarilene PORAWSKIBackground Renal failure is a frequent and serious complication in patients with decompensated cirrhosis. Objectives We aimed to evaluate the renal oxidative stress, cell damage and impaired cell function in animal model of cirrhosis. Methods Secondary biliary cirrhosis was induced in rats by ligation of the common bile duct. We measured TBARS, ROS and mitochondrial membrane potential in kidney as markers of oxidative stress, and activities of the antioxidant enzymes. Relative cell viability was determined by trypan blue dye-exclusion assay. Annexin V-PE was used with a vital dye, 7-AAD, to distinguish apoptotic from necrotic cells and comet assay was used for determined DNA integrity in single cells. Results In bile duct ligation animals there was significant increase in the kidney lipoperoxidation and an increase of the level of intracellular ROS. There was too an increase in the activity of all antioxidant enzymes evaluated in the kidney. The percentage viability was above 90% in the control group and in bile duct ligation was 64.66% and the dominant cell death type was apoptosis. DNA damage was observed in the bile duct ligation. There was a decreased in the mitochondrial membrane potential from 71.40% ± 6.35% to 34.48% ± 11.40% in bile duct ligation. Conclusions These results indicate that intracellular increase of ROS cause damage in the DNA and apoptosis getting worse the renal function in cirrhosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032015000100014&lng=en&tlng=enCirrose hepáticaInsuficiência renalEstresse oxidativoCitometria de fluxoEspécies reativas de oxigênio |
| spellingShingle | Keli Cristina Simões da SILVEIRA Cassiana Macagnan VIAU Josiane Raskopf COLARES Jenifer SAFFI Norma Possa MARRONI Marilene PORAWSKI CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS Arquivos de Gastroenterologia Cirrose hepática Insuficiência renal Estresse oxidativo Citometria de fluxo Espécies reativas de oxigênio |
| title | CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS |
| title_full | CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS |
| title_fullStr | CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS |
| title_full_unstemmed | CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS |
| title_short | CIRRHOSIS INDUCES APOPTOSIS IN RENAL TISSUE THROUGH INTRACELLULAR OXIDATIVE STRESS |
| title_sort | cirrhosis induces apoptosis in renal tissue through intracellular oxidative stress |
| topic | Cirrose hepática Insuficiência renal Estresse oxidativo Citometria de fluxo Espécies reativas de oxigênio |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0004-28032015000100014&lng=en&tlng=en |
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