Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding
Breeding of multi-stress tolerant rice varieties with higher grain yields is the best option to enhance the rice productivity of abiotic stresses prone areas. It also poses the greatest challenge to plant breeders to breed rice varieties for such stress prone conditions. Here, we carried out a desig...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2017.01275/full |
| _version_ | 1828475906157445120 |
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| author | Yunlong Pang Yunlong Pang Kai Chen Xiaoqian Wang Xiaoqian Wang Wensheng Wang Jianlong Xu Jianlong Xu Jianlong Xu Jauhar Ali Zhikang Li Zhikang Li |
| author_facet | Yunlong Pang Yunlong Pang Kai Chen Xiaoqian Wang Xiaoqian Wang Wensheng Wang Jianlong Xu Jianlong Xu Jianlong Xu Jauhar Ali Zhikang Li Zhikang Li |
| author_sort | Yunlong Pang |
| collection | DOAJ |
| description | Breeding of multi-stress tolerant rice varieties with higher grain yields is the best option to enhance the rice productivity of abiotic stresses prone areas. It also poses the greatest challenge to plant breeders to breed rice varieties for such stress prone conditions. Here, we carried out a designed QTL pyramiding experiment to develop high yielding “Green Super Rice” varieties with significantly improved tolerance to salt stress and grain yield. Using the F4 population derived from a cross between two selected introgression lines, we were able to develop six mostly homozygous promising high yielding lines with significantly improved salt tolerance and grain yield under optimal and/or saline conditions in 3 years. Simultaneous mapping using the same breeding population and tunable genotyping-by-sequencing technology, we identified three QTL affecting salt injury score and leaf chlorophyll content. By analyzing 32M SNP data of the grandparents and graphical genotypes of the parents, we discovered 87 positional candidate genes for salt tolerant QTL. According to their functional annotation, we inferred the most likely candidate genes. We demonstrated that designed QTL pyramiding is a powerful strategy for simultaneous improvement and genetic dissection of complex traits in rice. |
| first_indexed | 2024-12-11T06:20:51Z |
| format | Article |
| id | doaj.art-029cbaf97b7e4a9fb1c93acfb6e4b121 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-11T06:20:51Z |
| publishDate | 2017-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-029cbaf97b7e4a9fb1c93acfb6e4b1212022-12-22T01:17:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-07-01810.3389/fpls.2017.01275273909Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL PyramidingYunlong Pang0Yunlong Pang1Kai Chen2Xiaoqian Wang3Xiaoqian Wang4Wensheng Wang5Jianlong Xu6Jianlong Xu7Jianlong Xu8Jauhar Ali9Zhikang Li10Zhikang Li11Institute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural SciencesBeijing, ChinaInternational Rice Research InstituteMetro Manila, PhilippinesAgricultural Genomics Institute, Chinese Academy of Agricultural SciencesShenzhen, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural SciencesBeijing, ChinaInternational Rice Research InstituteMetro Manila, PhilippinesInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural SciencesBeijing, ChinaInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural SciencesBeijing, ChinaAgricultural Genomics Institute, Chinese Academy of Agricultural SciencesShenzhen, ChinaShenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural SciencesShenzhen, ChinaInternational Rice Research InstituteMetro Manila, PhilippinesInstitute of Crop Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural SciencesBeijing, ChinaShenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural SciencesShenzhen, ChinaBreeding of multi-stress tolerant rice varieties with higher grain yields is the best option to enhance the rice productivity of abiotic stresses prone areas. It also poses the greatest challenge to plant breeders to breed rice varieties for such stress prone conditions. Here, we carried out a designed QTL pyramiding experiment to develop high yielding “Green Super Rice” varieties with significantly improved tolerance to salt stress and grain yield. Using the F4 population derived from a cross between two selected introgression lines, we were able to develop six mostly homozygous promising high yielding lines with significantly improved salt tolerance and grain yield under optimal and/or saline conditions in 3 years. Simultaneous mapping using the same breeding population and tunable genotyping-by-sequencing technology, we identified three QTL affecting salt injury score and leaf chlorophyll content. By analyzing 32M SNP data of the grandparents and graphical genotypes of the parents, we discovered 87 positional candidate genes for salt tolerant QTL. According to their functional annotation, we inferred the most likely candidate genes. We demonstrated that designed QTL pyramiding is a powerful strategy for simultaneous improvement and genetic dissection of complex traits in rice.http://journal.frontiersin.org/article/10.3389/fpls.2017.01275/fulldesigned QTL pyramidinggreen super ricegrain yieldsalt tolerancetGBS |
| spellingShingle | Yunlong Pang Yunlong Pang Kai Chen Xiaoqian Wang Xiaoqian Wang Wensheng Wang Jianlong Xu Jianlong Xu Jianlong Xu Jauhar Ali Zhikang Li Zhikang Li Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding Frontiers in Plant Science designed QTL pyramiding green super rice grain yield salt tolerance tGBS |
| title | Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding |
| title_full | Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding |
| title_fullStr | Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding |
| title_full_unstemmed | Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding |
| title_short | Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding |
| title_sort | simultaneous improvement and genetic dissection of salt tolerance of rice oryza sativa l by designed qtl pyramiding |
| topic | designed QTL pyramiding green super rice grain yield salt tolerance tGBS |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2017.01275/full |
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