Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds
The quantitative real time-PCR (QRT-PCR) is a very sensitive method used to quantify mRNA level in gene expression analysis. Combining amplification, detection and quantification in a single step, allows a more accurate measurement compared to the traditional PCR end point analysis (Pfaffl, 2001; Bu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2010-01-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/664 |
| _version_ | 1818027988644528128 |
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| author | M. Cagnazzo S. Braglia R. Davoli |
| author_facet | M. Cagnazzo S. Braglia R. Davoli |
| author_sort | M. Cagnazzo |
| collection | DOAJ |
| description | The quantitative real time-PCR (QRT-PCR) is a very sensitive method used to quantify mRNA level in gene expression analysis. Combining amplification, detection and quantification in a single step, allows a more accurate measurement compared to the traditional PCR end point analysis (Pfaffl, 2001; Bustin, 2002). |
| first_indexed | 2024-12-10T04:56:39Z |
| format | Article |
| id | doaj.art-029e97aaf5624ef0ab77a1d69e1d9379 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-10T04:56:39Z |
| publishDate | 2010-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-029e97aaf5624ef0ab77a1d69e1d93792022-12-22T02:01:29ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-01-0142s919310.4081/ijas.2005.2s.91Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breedsM. CagnazzoS. BragliaR. DavoliThe quantitative real time-PCR (QRT-PCR) is a very sensitive method used to quantify mRNA level in gene expression analysis. Combining amplification, detection and quantification in a single step, allows a more accurate measurement compared to the traditional PCR end point analysis (Pfaffl, 2001; Bustin, 2002).http://www.aspajournal.it/index.php/ijas/article/view/664quantitative real time-PCR, gene expression, pig embryos, skeletal muscle genes |
| spellingShingle | M. Cagnazzo S. Braglia R. Davoli Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds Italian Journal of Animal Science quantitative real time-PCR, gene expression, pig embryos, skeletal muscle genes |
| title | Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds |
| title_full | Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds |
| title_fullStr | Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds |
| title_full_unstemmed | Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds |
| title_short | Quantitative Real Time PCR approach to study gene expression profile during prenatal growth of skeletal muscle in pig of Duroc and Pietrain breeds |
| title_sort | quantitative real time pcr approach to study gene expression profile during prenatal growth of skeletal muscle in pig of duroc and pietrain breeds |
| topic | quantitative real time-PCR, gene expression, pig embryos, skeletal muscle genes |
| url | http://www.aspajournal.it/index.php/ijas/article/view/664 |
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