Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector
Background: Echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus Echinococcus, family Taeniidae. We aimed to subclone antigen B gene in pQE-30 plasmid, its expression, and purification. Methods: We subcloned HI gene into p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2009-12-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/111 |
| _version_ | 1818403112600207360 |
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| author | N Taghipour M Bandepour R Pazoki A Haghighi MR Nazari Pouya B Kazemi |
| author_facet | N Taghipour M Bandepour R Pazoki A Haghighi MR Nazari Pouya B Kazemi |
| author_sort | N Taghipour |
| collection | DOAJ |
| description | Background: Echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus Echinococcus, family Taeniidae. We aimed to subclone antigen B gene in pQE-30 plasmid, its expression, and purification.
Methods: We subcloned HI gene into pQE-30 expression vector. The recombinant vector was transformed into E. coli, M15 and mass cultured. The subcloned gene was expressed by IPTG. Subcloning of gene was confirmed by both PCR and enzyme digestion.
Results: Production of recombinant protein was confirmed by SDS-PAGE. Western blot analysis was carried out by both His-Tag monoclonal Ab and human serum to estimate the expressed protein in E. coli cells. Recombinant protein was purified and its specificity was proved by Western blotting.
Conclusion: Production of this recombinant protein can increase sensitivity and specificity in serological test (ELISA). |
| first_indexed | 2024-12-14T08:19:05Z |
| format | Article |
| id | doaj.art-029f8d24def44634ac2f9cf60a6ad59b |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-14T08:19:05Z |
| publishDate | 2009-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-029f8d24def44634ac2f9cf60a6ad59b2022-12-21T23:09:50ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2009-12-0144Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression VectorN Taghipour0M Bandepour1R Pazoki2A Haghighi3MR Nazari Pouya4B Kazemi5 Background: Echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus Echinococcus, family Taeniidae. We aimed to subclone antigen B gene in pQE-30 plasmid, its expression, and purification. Methods: We subcloned HI gene into pQE-30 expression vector. The recombinant vector was transformed into E. coli, M15 and mass cultured. The subcloned gene was expressed by IPTG. Subcloning of gene was confirmed by both PCR and enzyme digestion. Results: Production of recombinant protein was confirmed by SDS-PAGE. Western blot analysis was carried out by both His-Tag monoclonal Ab and human serum to estimate the expressed protein in E. coli cells. Recombinant protein was purified and its specificity was proved by Western blotting. Conclusion: Production of this recombinant protein can increase sensitivity and specificity in serological test (ELISA).https://ijpa.tums.ac.ir/index.php/ijpa/article/view/111Cystic echinococcosisRecombinant antigen BHI gene |
| spellingShingle | N Taghipour M Bandepour R Pazoki A Haghighi MR Nazari Pouya B Kazemi Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector Iranian Journal of Parasitology Cystic echinococcosis Recombinant antigen B HI gene |
| title | Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector |
| title_full | Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector |
| title_fullStr | Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector |
| title_full_unstemmed | Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector |
| title_short | Subcloning and Expression of Recombinant Echinococcus granulosus Antigen B, in Pqe-30 Expression Vector |
| title_sort | subcloning and expression of recombinant echinococcus granulosus antigen b in pqe 30 expression vector |
| topic | Cystic echinococcosis Recombinant antigen B HI gene |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/111 |
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