Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma
A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480855/?tool=EBI |
| _version_ | 1818387353198133248 |
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| author | Lluc Farrera-Soler Jean-Pierre Daguer Sofia Barluenga Oscar Vadas Patrick Cohen Sabrina Pagano Sabine Yerly Laurent Kaiser Nicolas Vuilleumier Nicolas Winssinger Nicholas J. Mantis |
| author_facet | Lluc Farrera-Soler Jean-Pierre Daguer Sofia Barluenga Oscar Vadas Patrick Cohen Sabrina Pagano Sabine Yerly Laurent Kaiser Nicolas Vuilleumier Nicolas Winssinger Nicholas J. Mantis |
| author_sort | Lluc Farrera-Soler |
| collection | DOAJ |
| description | A novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2’) known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike. |
| first_indexed | 2024-12-14T04:08:36Z |
| format | Article |
| id | doaj.art-02a07d8dd0ea443a9c771cfdf1826917 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T04:08:36Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-02a07d8dd0ea443a9c771cfdf18269172022-12-21T23:17:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01159Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasmaLluc Farrera-SolerJean-Pierre DaguerSofia BarluengaOscar VadasPatrick CohenSabrina PaganoSabine YerlyLaurent KaiserNicolas VuilleumierNicolas WinssingerNicholas J. MantisA novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) is the source of a current pandemic (COVID-19) with devastating consequences in public health and economic stability. Using a peptide array to map the antibody response of plasma from healing patients (12) and heathy patients (6), we identified three immunodominant linear epitopes, two of which correspond to key proteolytic sites on the spike protein (S1/S2 and S2’) known to be critical for cellular entry. We show biochemical evidence that plasma positive for the epitope adjacent to the S1/S2 cleavage site inhibits furin-mediated proteolysis of spike.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480855/?tool=EBI |
| spellingShingle | Lluc Farrera-Soler Jean-Pierre Daguer Sofia Barluenga Oscar Vadas Patrick Cohen Sabrina Pagano Sabine Yerly Laurent Kaiser Nicolas Vuilleumier Nicolas Winssinger Nicholas J. Mantis Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma PLoS ONE |
| title | Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma |
| title_full | Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma |
| title_fullStr | Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma |
| title_full_unstemmed | Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma |
| title_short | Identification of immunodominant linear epitopes from SARS-CoV-2 patient plasma |
| title_sort | identification of immunodominant linear epitopes from sars cov 2 patient plasma |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7480855/?tool=EBI |
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