Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy Cows
Dietary lipids increase energy density in dairy cow diets and in some cases can increase beneficial fatty acids (FA) in milk and dairy products. However, the degree of FA saturation may affect the rumen microbiome. The objective of this study was to determine the long-term effects of feeding saturat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/6/1121 |
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| author | Nathaly Cancino-Padilla Natalia Catalán Karen Siu-Ting Christopher J. Creevey Sharon A. Huws Jaime Romero Einar Vargas-Bello-Pérez |
| author_facet | Nathaly Cancino-Padilla Natalia Catalán Karen Siu-Ting Christopher J. Creevey Sharon A. Huws Jaime Romero Einar Vargas-Bello-Pérez |
| author_sort | Nathaly Cancino-Padilla |
| collection | DOAJ |
| description | Dietary lipids increase energy density in dairy cow diets and in some cases can increase beneficial fatty acids (FA) in milk and dairy products. However, the degree of FA saturation may affect the rumen microbiome. The objective of this study was to determine the long-term effects of feeding saturated (hydrogenated vegetable oil; HVO) or unsaturated (olive oil; OO) fatty acid (FA) sources on the rumen microbiome of dairy cows. For 63 days, 15 mid-lactating cows were fed with either a basal diet (no fat supplement), or the basal diet supplemented with 3% dry matter (DM), either HVO or OO. Rumen contents were collected on days 21, 42 and 63 for 16S rRNA gene sequencing using the Illumina MiSeq platform. The results reveal dominance of the phyla Firmicutes (71.5%) and Bacteroidetes (26.2%), and their respective prevalent genera <i>Succiniclasticum</i> (19.4%) and <i>Prevotella</i> (16.6%). <i>Succiniclasticum</i> increased with both treatments at all time points. <i>Prevotella</i> was reduced on day 42 in both diets. Bacterial diversity alpha or beta were not affected by diets. Predicted bacterial functions by CowPI showed changes in energy and protein metabolism. Overall, 3% DM of lipid supplementation over 63 days can be used in dairy cow diets without major impacts on global bacterial community structure. |
| first_indexed | 2024-03-10T11:08:56Z |
| format | Article |
| id | doaj.art-02ab5f3f511e4594b24680f8664f7589 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:08:56Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-02ab5f3f511e4594b24680f8664f75892023-11-21T20:54:20ZengMDPI AGMicroorganisms2076-26072021-05-0196112110.3390/microorganisms9061121Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy CowsNathaly Cancino-Padilla0Natalia Catalán1Karen Siu-Ting2Christopher J. Creevey3Sharon A. Huws4Jaime Romero5Einar Vargas-Bello-Pérez6Departamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Avda. Vicuña Mackenna 4860, Santiago 6904411, ChileLaboratorio de Biotecnología de Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, El Líbano 5524, Macul, Santiago 7830490, ChileInstitute for Global Food Security, School of Biological Sciences, Queen’s University of Belfast, 19 Chlorine Gardens, Belfast BT9 5DL, UKInstitute for Global Food Security, School of Biological Sciences, Queen’s University of Belfast, 19 Chlorine Gardens, Belfast BT9 5DL, UKInstitute for Global Food Security, School of Biological Sciences, Queen’s University of Belfast, 19 Chlorine Gardens, Belfast BT9 5DL, UKLaboratorio de Biotecnología de Alimentos, Unidad de Alimentos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, El Líbano 5524, Macul, Santiago 7830490, ChileDepartamento de Ciencias Animales, Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Avda. Vicuña Mackenna 4860, Santiago 6904411, ChileDietary lipids increase energy density in dairy cow diets and in some cases can increase beneficial fatty acids (FA) in milk and dairy products. However, the degree of FA saturation may affect the rumen microbiome. The objective of this study was to determine the long-term effects of feeding saturated (hydrogenated vegetable oil; HVO) or unsaturated (olive oil; OO) fatty acid (FA) sources on the rumen microbiome of dairy cows. For 63 days, 15 mid-lactating cows were fed with either a basal diet (no fat supplement), or the basal diet supplemented with 3% dry matter (DM), either HVO or OO. Rumen contents were collected on days 21, 42 and 63 for 16S rRNA gene sequencing using the Illumina MiSeq platform. The results reveal dominance of the phyla Firmicutes (71.5%) and Bacteroidetes (26.2%), and their respective prevalent genera <i>Succiniclasticum</i> (19.4%) and <i>Prevotella</i> (16.6%). <i>Succiniclasticum</i> increased with both treatments at all time points. <i>Prevotella</i> was reduced on day 42 in both diets. Bacterial diversity alpha or beta were not affected by diets. Predicted bacterial functions by CowPI showed changes in energy and protein metabolism. Overall, 3% DM of lipid supplementation over 63 days can be used in dairy cow diets without major impacts on global bacterial community structure.https://www.mdpi.com/2076-2607/9/6/1121rumenbacteria16S rDNAmicrobiomeolive oilpalm oil |
| spellingShingle | Nathaly Cancino-Padilla Natalia Catalán Karen Siu-Ting Christopher J. Creevey Sharon A. Huws Jaime Romero Einar Vargas-Bello-Pérez Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy Cows Microorganisms rumen bacteria 16S rDNA microbiome olive oil palm oil |
| title | Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy Cows |
| title_full | Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy Cows |
| title_fullStr | Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy Cows |
| title_full_unstemmed | Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy Cows |
| title_short | Long-Term Effects of Dietary Supplementation with Olive Oil and Hydrogenated Vegetable Oil on the Rumen Microbiome of Dairy Cows |
| title_sort | long term effects of dietary supplementation with olive oil and hydrogenated vegetable oil on the rumen microbiome of dairy cows |
| topic | rumen bacteria 16S rDNA microbiome olive oil palm oil |
| url | https://www.mdpi.com/2076-2607/9/6/1121 |
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