The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats
Background & aim: Conazoles including imidazoles or triazoles are anti- fungal agents widely used to prevent fungal growth and their infections. Propiconazole placed in this group is a systemic fungicide used widely for detoxification of cereal seeds especially rice in Iran and other countries....
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| Format: | Article |
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Yasuj University Of Medical Sciences
2016-08-01
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| Series: | Armaghane Danesh Bimonthly Journal |
| Subjects: | |
| Online Access: | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-666-1&slc_lang=en&sid=1 |
| _version_ | 1818024613474467840 |
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| author | S Rashidi pouya H Mohseni kouchesfehani SA Angaji |
| author_facet | S Rashidi pouya H Mohseni kouchesfehani SA Angaji |
| author_sort | S Rashidi pouya |
| collection | DOAJ |
| description | Background & aim: Conazoles including imidazoles or triazoles are anti- fungal agents widely used to prevent fungal growth and their infections. Propiconazole placed in this group is a systemic fungicide used widely for detoxification of cereal seeds especially rice in Iran and other countries. This fungicide were designed to inhibit a specific cytochrome P450, CYP51 (lanosterol-14-α- demethylase), a critical step in the biosynthesis of ergosterol, a steroid required for the formation of the fungal cell wall. In the present experimental study, the effect of propiconazole on Caspase 9 gene expression profile as an initiator of apoptotic process and protective effect of selenium were investigated.
Methods: Forty SD rats were divided into 10 groups of 4, including : control , sham1 (solvent of propiconazole, distilled water), sham 2 (solvent of selenium, normal saline) and 1 group received 0.5 mg/kg selenium ,3 groups received propiconazole in doses of 10,50,75 mg/kg and 3 groups received propiconazole in doses of 10,50,75 mg/kg propiconazole with 0.5 mg/kg of selenium. Injections were intrapritoneal for two weeks in alternate days. Then, using RT-PCR and Total Lab program gene expression of caspase-9 testicular of all groups were studied. Data were analyzed using descriptive statistics.
Results: A significant increase of caspase 9 expression were observed among all experimental groups compared to control and sham groups. These findings indicated that 0.5 mg/kg selenium is not a suitable dose to create protection in this experimental study.
Conclusion: The significant increase in Caspase 9 gene expression profile observed in all experimental groups as compared to control suggests activation of apoptosis and inefficacy of selenium to protect the testis against induced damages. |
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| issn | 1728-6514 1728-6506 |
| language | fas |
| last_indexed | 2024-12-10T04:03:00Z |
| publishDate | 2016-08-01 |
| publisher | Yasuj University Of Medical Sciences |
| record_format | Article |
| series | Armaghane Danesh Bimonthly Journal |
| spelling | doaj.art-02ac93140f43469aa11c2552f7707e9e2022-12-22T02:02:56ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65141728-65062016-08-01215435445The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) RatsS Rashidi pouya0H Mohseni kouchesfehani1SA Angaji2 Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Department of Cell & Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran Background & aim: Conazoles including imidazoles or triazoles are anti- fungal agents widely used to prevent fungal growth and their infections. Propiconazole placed in this group is a systemic fungicide used widely for detoxification of cereal seeds especially rice in Iran and other countries. This fungicide were designed to inhibit a specific cytochrome P450, CYP51 (lanosterol-14-α- demethylase), a critical step in the biosynthesis of ergosterol, a steroid required for the formation of the fungal cell wall. In the present experimental study, the effect of propiconazole on Caspase 9 gene expression profile as an initiator of apoptotic process and protective effect of selenium were investigated. Methods: Forty SD rats were divided into 10 groups of 4, including : control , sham1 (solvent of propiconazole, distilled water), sham 2 (solvent of selenium, normal saline) and 1 group received 0.5 mg/kg selenium ,3 groups received propiconazole in doses of 10,50,75 mg/kg and 3 groups received propiconazole in doses of 10,50,75 mg/kg propiconazole with 0.5 mg/kg of selenium. Injections were intrapritoneal for two weeks in alternate days. Then, using RT-PCR and Total Lab program gene expression of caspase-9 testicular of all groups were studied. Data were analyzed using descriptive statistics. Results: A significant increase of caspase 9 expression were observed among all experimental groups compared to control and sham groups. These findings indicated that 0.5 mg/kg selenium is not a suitable dose to create protection in this experimental study. Conclusion: The significant increase in Caspase 9 gene expression profile observed in all experimental groups as compared to control suggests activation of apoptosis and inefficacy of selenium to protect the testis against induced damages.http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-666-1&slc_lang=en&sid=1Apoptosis CASP 9 Propiconazole SD rat Selenium |
| spellingShingle | S Rashidi pouya H Mohseni kouchesfehani SA Angaji The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats Armaghane Danesh Bimonthly Journal Apoptosis CASP 9 Propiconazole SD rat Selenium |
| title | The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats |
| title_full | The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats |
| title_fullStr | The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats |
| title_full_unstemmed | The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats |
| title_short | The Effect of Propiconazole and Protective Effects of Selenium Gene Expression Profile of Caspase 9 in the Testicular Tissue of Male Sprague Dawley (SD) Rats |
| title_sort | effect of propiconazole and protective effects of selenium gene expression profile of caspase 9 in the testicular tissue of male sprague dawley sd rats |
| topic | Apoptosis CASP 9 Propiconazole SD rat Selenium |
| url | http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-666-1&slc_lang=en&sid=1 |
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