Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs
The fundamental role of any neuron within a network is to transform complex spatiotemporal synaptic input patterns into individual output spikes. These spikes, in turn, act as inputs for other neurons in the network. Neurons must execute this function across a diverse range of physiological conditio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2023.1281932/full |
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| author | Paola Vitale Fabio Librizzi Andrea C. Vaiana Elisa Capuana Maurizio Pezzoli Maurizio Pezzoli Ying Shi Ying Shi Armando Romani Michele Migliore Rosanna Migliore |
| author_facet | Paola Vitale Fabio Librizzi Andrea C. Vaiana Elisa Capuana Maurizio Pezzoli Maurizio Pezzoli Ying Shi Ying Shi Armando Romani Michele Migliore Rosanna Migliore |
| author_sort | Paola Vitale |
| collection | DOAJ |
| description | The fundamental role of any neuron within a network is to transform complex spatiotemporal synaptic input patterns into individual output spikes. These spikes, in turn, act as inputs for other neurons in the network. Neurons must execute this function across a diverse range of physiological conditions, often based on species-specific traits. Therefore, it is crucial to determine the extent to which findings can be extrapolated between species and, ultimately, to humans. In this study, we employed a multidisciplinary approach to pinpoint the factors accounting for the observed electrophysiological differences between mice and rats, the two species most used in experimental and computational research. After analyzing the morphological properties of their hippocampal CA1 pyramidal cells, we conducted a statistical comparison of rat and mouse electrophysiological features in response to somatic current injections. This analysis aimed to uncover the parameters underlying these distinctions. Using a well-established computational workflow, we created ten distinct single-cell computational models of mouse CA1 pyramidal neurons, ready to be used in a full-scale hippocampal circuit. By comparing their responses to a variety of somatic and synaptic inputs with those of rat models, we generated experimentally testable hypotheses regarding species-specific differences in ion channel distribution, kinetics, and the electrophysiological mechanisms underlying their distinct responses to synaptic inputs during the behaviorally relevant Gamma and Sharp-Wave rhythms. |
| first_indexed | 2024-03-09T01:59:18Z |
| format | Article |
| id | doaj.art-02ad6e22254048d2aa16beb2b99abb6b |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-03-09T01:59:18Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-02ad6e22254048d2aa16beb2b99abb6b2023-12-08T05:48:26ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022023-12-011710.3389/fncel.2023.12819321281932Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputsPaola Vitale0Fabio Librizzi1Andrea C. Vaiana2Elisa Capuana3Maurizio Pezzoli4Maurizio Pezzoli5Ying Shi6Ying Shi7Armando Romani8Michele Migliore9Rosanna Migliore10Institute of Biophysics, National Research Council, Palermo, ItalyInstitute of Biophysics, National Research Council, Palermo, ItalyInstitute of Biophysics, National Research Council, Palermo, ItalyInstitute of Biophysics, National Research Council, Palermo, ItalyLaboratory of Neural Microcircuitry, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, SwitzerlandBlue Brain Project, École Polytechnique Fédérale de Lausanne (EPFL), Campus Biotech, Geneva, SwitzerlandLaboratory of Neural Microcircuitry, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, SwitzerlandBlue Brain Project, École Polytechnique Fédérale de Lausanne (EPFL), Campus Biotech, Geneva, SwitzerlandBlue Brain Project, École Polytechnique Fédérale de Lausanne (EPFL), Campus Biotech, Geneva, SwitzerlandInstitute of Biophysics, National Research Council, Palermo, ItalyInstitute of Biophysics, National Research Council, Palermo, ItalyThe fundamental role of any neuron within a network is to transform complex spatiotemporal synaptic input patterns into individual output spikes. These spikes, in turn, act as inputs for other neurons in the network. Neurons must execute this function across a diverse range of physiological conditions, often based on species-specific traits. Therefore, it is crucial to determine the extent to which findings can be extrapolated between species and, ultimately, to humans. In this study, we employed a multidisciplinary approach to pinpoint the factors accounting for the observed electrophysiological differences between mice and rats, the two species most used in experimental and computational research. After analyzing the morphological properties of their hippocampal CA1 pyramidal cells, we conducted a statistical comparison of rat and mouse electrophysiological features in response to somatic current injections. This analysis aimed to uncover the parameters underlying these distinctions. Using a well-established computational workflow, we created ten distinct single-cell computational models of mouse CA1 pyramidal neurons, ready to be used in a full-scale hippocampal circuit. By comparing their responses to a variety of somatic and synaptic inputs with those of rat models, we generated experimentally testable hypotheses regarding species-specific differences in ion channel distribution, kinetics, and the electrophysiological mechanisms underlying their distinct responses to synaptic inputs during the behaviorally relevant Gamma and Sharp-Wave rhythms.https://www.frontiersin.org/articles/10.3389/fncel.2023.1281932/fullpyramidal neuronshippocampusrodent comparisonelectrophysiological featurescomputational modeling |
| spellingShingle | Paola Vitale Fabio Librizzi Andrea C. Vaiana Elisa Capuana Maurizio Pezzoli Maurizio Pezzoli Ying Shi Ying Shi Armando Romani Michele Migliore Rosanna Migliore Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs Frontiers in Cellular Neuroscience pyramidal neurons hippocampus rodent comparison electrophysiological features computational modeling |
| title | Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs |
| title_full | Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs |
| title_fullStr | Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs |
| title_full_unstemmed | Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs |
| title_short | Different responses of mice and rats hippocampus CA1 pyramidal neurons to in vitro and in vivo-like inputs |
| title_sort | different responses of mice and rats hippocampus ca1 pyramidal neurons to in vitro and in vivo like inputs |
| topic | pyramidal neurons hippocampus rodent comparison electrophysiological features computational modeling |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2023.1281932/full |
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