KIR- Ligand Interactions in Hypertensive Disorders in Pregnancy
HypothesisThe activity of natural killer (NK) cells is considered an important factor for the tolerance of the fetus during pregnancy. The complications of pregnancy, such as hypertensive disorders (HDP), may be therefore associated with this immune compartment.MethodsThe current study included 41 p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.868175/full |
| _version_ | 1811291422663376896 |
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| author | Katarzyna Stefańska Martyna Tomaszewicz Joanna Dębska-Zielkowska Dorota Zamkowska Karolina Piekarska Justyna Sakowska Maciej Studziński Bogusław Tymoniuk Przemysław Adamski Joanna Jassem-Bobowicz Piotr Wydra Katarzyna Leszczyńska Renata Świątkowska-Stodulska Sebastian Kwiatkowski Krzysztof Preis Piotr Trzonkowski Natalia Marek-Trzonkowska Natalia Marek-Trzonkowska Maciej Zieliński |
| author_facet | Katarzyna Stefańska Martyna Tomaszewicz Joanna Dębska-Zielkowska Dorota Zamkowska Karolina Piekarska Justyna Sakowska Maciej Studziński Bogusław Tymoniuk Przemysław Adamski Joanna Jassem-Bobowicz Piotr Wydra Katarzyna Leszczyńska Renata Świątkowska-Stodulska Sebastian Kwiatkowski Krzysztof Preis Piotr Trzonkowski Natalia Marek-Trzonkowska Natalia Marek-Trzonkowska Maciej Zieliński |
| author_sort | Katarzyna Stefańska |
| collection | DOAJ |
| description | HypothesisThe activity of natural killer (NK) cells is considered an important factor for the tolerance of the fetus during pregnancy. The complications of pregnancy, such as hypertensive disorders (HDP), may be therefore associated with this immune compartment.MethodsThe current study included 41 pregnant women diagnosed with HDPs (Gestational Hypertension; GH or Preeclampsia; PE) and 21 healthy women. All the patients were under continuous obstetric care during the pregnancy and labour. The number of mother-child mismatches within killer immunoglobulin-like receptors (KIRs), their ligands [MM], and missing KIR ligands [MSLs] was assessed. KIRs and their ligands were assessed with Next Generation Sequencing (NGS) and Polymerase Chain Reaction Sequence-Specific Oligonucleotide (PCR-SSO) typing. The subsets of NK cells were assessed with multicolor flow cytometry and correlated to the number of MSLs.ResultsThe number of MSLs was significantly higher in HDP patients when compared to healthy non-complicated pregnancy patients. Some MSLs, such as those with 2DS2 activating KIR, were present only in HDP patients. The percentage of CD56+CD16-CD94+ NK cells and CD56+CD16-CD279+ NK cells correlated with the number of MSLs with inhibiting KIRs only in healthy patients. In HDP patients, there was a correlation between the percentage of CD56-CD16+CD69+ NK cells and the number of MSLs with inhibiting and activating KIRs. As compared to the healthy group, the percentage of CD56+CD16-CD279+ NK cells and CD56-CD16+CD279+ NK cells were lower in HDP patients. HDP patients were also characterized by a higher percentage of CD56+CD16+perforin+ NK cells than their healthy counterparts.ConclusionsPatients with HDP were characterized by a higher number of MSLs within the KIRs receptors. It seemed that the number of MSLs in the healthy group was balanced by various receptors, such as CD94 or inhibitory CD279, expressed on NK cells. Conversely, in HDP patients the number of MSLs was associated with the activation detected as the increased level of CD69+ NK cells. |
| first_indexed | 2024-04-13T04:29:09Z |
| format | Article |
| id | doaj.art-02b24d5798c240a2bd6bdd0eed5c0c09 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-13T04:29:09Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-02b24d5798c240a2bd6bdd0eed5c0c092022-12-22T03:02:23ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-07-011310.3389/fimmu.2022.868175868175KIR- Ligand Interactions in Hypertensive Disorders in PregnancyKatarzyna Stefańska0Martyna Tomaszewicz1Joanna Dębska-Zielkowska2Dorota Zamkowska3Karolina Piekarska4Justyna Sakowska5Maciej Studziński6Bogusław Tymoniuk7Przemysław Adamski8Joanna Jassem-Bobowicz9Piotr Wydra10Katarzyna Leszczyńska11Renata Świątkowska-Stodulska12Sebastian Kwiatkowski13Krzysztof Preis14Piotr Trzonkowski15Natalia Marek-Trzonkowska16Natalia Marek-Trzonkowska17Maciej Zieliński18Division of Gynecology and Obstetrics, Medical University of Gdansk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDivision of Gynecology and Obstetrics, Medical University of Gdansk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Immunology and Allergy, Medical University of Lodz, Łódź, PolandDivision of Gynecology and Obstetrics, Medical University of Gdansk, Gdańsk, PolandDepartment of Neonatology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDivision of Gynecology and Obstetrics, Medical University of Gdansk, Gdańsk, PolandDepartment of Endocrinology and Internal Medicine, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Obstetrics and Gynecology, Pomeranian Medical University of Szczecin, Szczecin, PolandDivision of Gynecology and Obstetrics, Medical University of Gdansk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandInternational Centre for Cancer Vaccine Science Cancer Immunology Group, University of Gdansk, Gdańsk, PolandLaboratory of Immunoregulation and Cellular Therapies, Department of Family Medicine, Medical University of Gdańsk, Gdańsk, PolandDepartment of Medical Immunology, Faculty of Medicine, Medical University of Gdańsk, Gdańsk, PolandHypothesisThe activity of natural killer (NK) cells is considered an important factor for the tolerance of the fetus during pregnancy. The complications of pregnancy, such as hypertensive disorders (HDP), may be therefore associated with this immune compartment.MethodsThe current study included 41 pregnant women diagnosed with HDPs (Gestational Hypertension; GH or Preeclampsia; PE) and 21 healthy women. All the patients were under continuous obstetric care during the pregnancy and labour. The number of mother-child mismatches within killer immunoglobulin-like receptors (KIRs), their ligands [MM], and missing KIR ligands [MSLs] was assessed. KIRs and their ligands were assessed with Next Generation Sequencing (NGS) and Polymerase Chain Reaction Sequence-Specific Oligonucleotide (PCR-SSO) typing. The subsets of NK cells were assessed with multicolor flow cytometry and correlated to the number of MSLs.ResultsThe number of MSLs was significantly higher in HDP patients when compared to healthy non-complicated pregnancy patients. Some MSLs, such as those with 2DS2 activating KIR, were present only in HDP patients. The percentage of CD56+CD16-CD94+ NK cells and CD56+CD16-CD279+ NK cells correlated with the number of MSLs with inhibiting KIRs only in healthy patients. In HDP patients, there was a correlation between the percentage of CD56-CD16+CD69+ NK cells and the number of MSLs with inhibiting and activating KIRs. As compared to the healthy group, the percentage of CD56+CD16-CD279+ NK cells and CD56-CD16+CD279+ NK cells were lower in HDP patients. HDP patients were also characterized by a higher percentage of CD56+CD16+perforin+ NK cells than their healthy counterparts.ConclusionsPatients with HDP were characterized by a higher number of MSLs within the KIRs receptors. It seemed that the number of MSLs in the healthy group was balanced by various receptors, such as CD94 or inhibitory CD279, expressed on NK cells. Conversely, in HDP patients the number of MSLs was associated with the activation detected as the increased level of CD69+ NK cells.https://www.frontiersin.org/articles/10.3389/fimmu.2022.868175/fullhypertensive disorder complicating pregnancyKIR HLA ligandnatural killer (NK)immunologygestational hypertension (GH)preeclampsia (PE) |
| spellingShingle | Katarzyna Stefańska Martyna Tomaszewicz Joanna Dębska-Zielkowska Dorota Zamkowska Karolina Piekarska Justyna Sakowska Maciej Studziński Bogusław Tymoniuk Przemysław Adamski Joanna Jassem-Bobowicz Piotr Wydra Katarzyna Leszczyńska Renata Świątkowska-Stodulska Sebastian Kwiatkowski Krzysztof Preis Piotr Trzonkowski Natalia Marek-Trzonkowska Natalia Marek-Trzonkowska Maciej Zieliński KIR- Ligand Interactions in Hypertensive Disorders in Pregnancy Frontiers in Immunology hypertensive disorder complicating pregnancy KIR HLA ligand natural killer (NK) immunology gestational hypertension (GH) preeclampsia (PE) |
| title | KIR- Ligand Interactions in Hypertensive Disorders in Pregnancy |
| title_full | KIR- Ligand Interactions in Hypertensive Disorders in Pregnancy |
| title_fullStr | KIR- Ligand Interactions in Hypertensive Disorders in Pregnancy |
| title_full_unstemmed | KIR- Ligand Interactions in Hypertensive Disorders in Pregnancy |
| title_short | KIR- Ligand Interactions in Hypertensive Disorders in Pregnancy |
| title_sort | kir ligand interactions in hypertensive disorders in pregnancy |
| topic | hypertensive disorder complicating pregnancy KIR HLA ligand natural killer (NK) immunology gestational hypertension (GH) preeclampsia (PE) |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.868175/full |
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