Myo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodes
Abstract Cell migration is a dynamic process that involves adhesion molecules and the deformation of the moving cell that depends on cytoskeletal remodeling and actin-modulating proteins such as myosins. In this work, we analyzed the role of the class I Myosin-1 g (Myo1g) in migratory processes of L...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-85477-y |
| _version_ | 1818683807131238400 |
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| author | D. Cruz-Zárate O. López-Ortega D. A. Girón-Pérez A. M. Gonzalez-Suarez J. L. García-Cordero M. Schnoor L. Santos-Argumedo |
| author_facet | D. Cruz-Zárate O. López-Ortega D. A. Girón-Pérez A. M. Gonzalez-Suarez J. L. García-Cordero M. Schnoor L. Santos-Argumedo |
| author_sort | D. Cruz-Zárate |
| collection | DOAJ |
| description | Abstract Cell migration is a dynamic process that involves adhesion molecules and the deformation of the moving cell that depends on cytoskeletal remodeling and actin-modulating proteins such as myosins. In this work, we analyzed the role of the class I Myosin-1 g (Myo1g) in migratory processes of LPS + IL-4 activated B lymphocytes in vivo and in vitro. In vivo, the absence of Myo1g reduced homing of activated B lymphocytes into the inguinal lymph node. Using microchannel chambers and morphology analysis, we found that the lack of Myo1g caused adhesion and chemotaxis defects. Additionally, deficiency in Myo1g causes flaws in adopting a migratory morphology. Our results highlight the importance of Myo1g during B cell migration. |
| first_indexed | 2024-12-17T10:40:36Z |
| format | Article |
| id | doaj.art-02b8ea5efc7943298d716d4ed4d77750 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T10:40:36Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-02b8ea5efc7943298d716d4ed4d777502022-12-21T21:52:15ZengNature PortfolioScientific Reports2045-23222021-03-0111111210.1038/s41598-021-85477-yMyo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodesD. Cruz-Zárate0O. López-Ortega1D. A. Girón-Pérez2A. M. Gonzalez-Suarez3J. L. García-Cordero4M. Schnoor5L. Santos-Argumedo6Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)Unidad Monterrey, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)Unidad Monterrey, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN)Abstract Cell migration is a dynamic process that involves adhesion molecules and the deformation of the moving cell that depends on cytoskeletal remodeling and actin-modulating proteins such as myosins. In this work, we analyzed the role of the class I Myosin-1 g (Myo1g) in migratory processes of LPS + IL-4 activated B lymphocytes in vivo and in vitro. In vivo, the absence of Myo1g reduced homing of activated B lymphocytes into the inguinal lymph node. Using microchannel chambers and morphology analysis, we found that the lack of Myo1g caused adhesion and chemotaxis defects. Additionally, deficiency in Myo1g causes flaws in adopting a migratory morphology. Our results highlight the importance of Myo1g during B cell migration.https://doi.org/10.1038/s41598-021-85477-y |
| spellingShingle | D. Cruz-Zárate O. López-Ortega D. A. Girón-Pérez A. M. Gonzalez-Suarez J. L. García-Cordero M. Schnoor L. Santos-Argumedo Myo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodes Scientific Reports |
| title | Myo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodes |
| title_full | Myo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodes |
| title_fullStr | Myo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodes |
| title_full_unstemmed | Myo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodes |
| title_short | Myo1g is required for efficient adhesion and migration of activated B lymphocytes to inguinal lymph nodes |
| title_sort | myo1g is required for efficient adhesion and migration of activated b lymphocytes to inguinal lymph nodes |
| url | https://doi.org/10.1038/s41598-021-85477-y |
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