Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with Chemometrics
Wuyi Rock tea, well-recognized for rich flavor and long-lasting fragrance, is a premium subcategory of oolong tea mainly produced in Wuyi Mountain and nearby regions of China. The quality of tea is mainly determined by the chemical constituents in the tea leaves. However, this remains underexplored...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/2/104 |
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| author | Si Chen Meihong Li Gongyu Zheng Tingting Wang Jun Lin Shanshan Wang Xiaxia Wang Qianlin Chao Shixian Cao Zhenbiao Yang Xiaomin Yu |
| author_facet | Si Chen Meihong Li Gongyu Zheng Tingting Wang Jun Lin Shanshan Wang Xiaxia Wang Qianlin Chao Shixian Cao Zhenbiao Yang Xiaomin Yu |
| author_sort | Si Chen |
| collection | DOAJ |
| description | Wuyi Rock tea, well-recognized for rich flavor and long-lasting fragrance, is a premium subcategory of oolong tea mainly produced in Wuyi Mountain and nearby regions of China. The quality of tea is mainly determined by the chemical constituents in the tea leaves. However, this remains underexplored for Wuyi Rock tea cultivars. In this study, we investigated the leaf metabolite profiles of 14 major Wuyi Rock tea cultivars grown in the same producing region using UPLC-QTOF MS and UPLC-QqQ MS with data processing via principal component analysis and cluster analysis. Relative quantitation of 49 major metabolites including flavan-3-ols, proanthocyanidins, flavonol glycosides, flavone glycosides, flavonone glycosides, phenolic acid derivatives, hydrolysable tannins, alkaloids and amino acids revealed clear variations between tea cultivars. In particular, catechins, kaempferol and quercetin derivatives were key metabolites responsible for cultivar discrimination. Information on the varietal differences in the levels of bioactive/functional metabolites, such as methylated catechins, flavonol glycosides and theanine, offers valuable insights to further explore the nutritional values and sensory qualities of Wuyi Rock tea. It also provides potential markers for tea plant fingerprinting and cultivar identification. |
| first_indexed | 2024-12-12T08:26:25Z |
| format | Article |
| id | doaj.art-02c2a3f7af3f466dbca2e37c8c3a7cf5 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-12T08:26:25Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-02c2a3f7af3f466dbca2e37c8c3a7cf52022-12-22T00:31:14ZengMDPI AGMolecules1420-30492018-01-0123210410.3390/molecules23020104molecules23020104Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with ChemometricsSi Chen0Meihong Li1Gongyu Zheng2Tingting Wang3Jun Lin4Shanshan Wang5Xiaxia Wang6Qianlin Chao7Shixian Cao8Zhenbiao Yang9Xiaomin Yu10College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaFAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaWuyi Star Tea Industry Co., Ltd., Wuyishan 354300, ChinaWuyi Star Tea Industry Co., Ltd., Wuyishan 354300, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaWuyi Rock tea, well-recognized for rich flavor and long-lasting fragrance, is a premium subcategory of oolong tea mainly produced in Wuyi Mountain and nearby regions of China. The quality of tea is mainly determined by the chemical constituents in the tea leaves. However, this remains underexplored for Wuyi Rock tea cultivars. In this study, we investigated the leaf metabolite profiles of 14 major Wuyi Rock tea cultivars grown in the same producing region using UPLC-QTOF MS and UPLC-QqQ MS with data processing via principal component analysis and cluster analysis. Relative quantitation of 49 major metabolites including flavan-3-ols, proanthocyanidins, flavonol glycosides, flavone glycosides, flavonone glycosides, phenolic acid derivatives, hydrolysable tannins, alkaloids and amino acids revealed clear variations between tea cultivars. In particular, catechins, kaempferol and quercetin derivatives were key metabolites responsible for cultivar discrimination. Information on the varietal differences in the levels of bioactive/functional metabolites, such as methylated catechins, flavonol glycosides and theanine, offers valuable insights to further explore the nutritional values and sensory qualities of Wuyi Rock tea. It also provides potential markers for tea plant fingerprinting and cultivar identification.http://www.mdpi.com/1420-3049/23/2/104Wuyi Rock teaqualityUPLC-QTOF MSUPLC-QqQ MSmetabolite profilingmetabolomicscluster analysiscultivars |
| spellingShingle | Si Chen Meihong Li Gongyu Zheng Tingting Wang Jun Lin Shanshan Wang Xiaxia Wang Qianlin Chao Shixian Cao Zhenbiao Yang Xiaomin Yu Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with Chemometrics Molecules Wuyi Rock tea quality UPLC-QTOF MS UPLC-QqQ MS metabolite profiling metabolomics cluster analysis cultivars |
| title | Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with Chemometrics |
| title_full | Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with Chemometrics |
| title_fullStr | Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with Chemometrics |
| title_full_unstemmed | Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with Chemometrics |
| title_short | Metabolite Profiling of 14 Wuyi Rock Tea Cultivars Using UPLC-QTOF MS and UPLC-QqQ MS Combined with Chemometrics |
| title_sort | metabolite profiling of 14 wuyi rock tea cultivars using uplc qtof ms and uplc qqq ms combined with chemometrics |
| topic | Wuyi Rock tea quality UPLC-QTOF MS UPLC-QqQ MS metabolite profiling metabolomics cluster analysis cultivars |
| url | http://www.mdpi.com/1420-3049/23/2/104 |
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