Testing patients with uveal melanoma for herpesvirus infections

Results of comprehensive ELISA tests of blood serum for the presence of IgM-, IgA-, and IgG-antibodies to herpes simplex virus types 1 and 2, cytomegalovirus, Epstein-Barr virus, human herpesvirus 6, human herpes virus 8 type, Chlamydia trachomatis in 38 patients with uveal melanoma are presented. T...

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Main Authors: S. V. Saakyan, E. B. Myakoshina, G. I. Krichevskaya, O. S. Slepova, O. G. Panteleeva, A. E. Andryushin, I. P. Khoroshilova, G. P. Zakharova
Format: Article
Language:English
Published: Central Research Institute for Epidemiology 2016-12-01
Series:Вопросы вирусологии
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Online Access:https://virusjour.crie.ru/jour/article/viewFile/109/40
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author S. V. Saakyan
E. B. Myakoshina
G. I. Krichevskaya
O. S. Slepova
O. G. Panteleeva
A. E. Andryushin
I. P. Khoroshilova
G. P. Zakharova
author_facet S. V. Saakyan
E. B. Myakoshina
G. I. Krichevskaya
O. S. Slepova
O. G. Panteleeva
A. E. Andryushin
I. P. Khoroshilova
G. P. Zakharova
author_sort S. V. Saakyan
collection DOAJ
description Results of comprehensive ELISA tests of blood serum for the presence of IgM-, IgA-, and IgG-antibodies to herpes simplex virus types 1 and 2, cytomegalovirus, Epstein-Barr virus, human herpesvirus 6, human herpes virus 8 type, Chlamydia trachomatis in 38 patients with uveal melanoma are presented. The polymerase chain reaction was used to detect DNA of these pathogens in tumor biopsies, vitreous body of 10 enucleated eyes, as well as in plasma IgG-antibodies to HHV 6 were revealed in 50% of patients; IgG-antibodies to HHV 8, in 5.3% of patients. Among the 16 patients with uveal melanoma at advanced stages, 6 patients had antibodies indicative of EBV reactivation (1.2-3.3). Chlamydia trachomatis genome was detected in both biopsies; in one of them, in conjunction with EBV and CMV DNA . Tissue samples from the identified infectious agents were related only to the spindle-cell histologic type AB of uveal melanoma. In plasma, genomes of pathogens were not determined. The results indicate the presence of infectious agents in patients with uveal melanoma and require further study of the pathogenetic role of infections in the pathogenesis of uveal melanoma.
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spelling doaj.art-02c507e5d1fe4d0384dc4f66f0c86dcf2023-07-12T20:03:24ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972016-12-0161628428710.18821/0507-4088-2016-61-6-284-28790Testing patients with uveal melanoma for herpesvirus infectionsS. V. Saakyan0E. B. Myakoshina1G. I. Krichevskaya2O. S. Slepova3O. G. Panteleeva4A. E. Andryushin5I. P. Khoroshilova6G. P. Zakharova7Helmholtz Moscow Research Institute of Eye DiseasesHelmholtz Moscow Research Institute of Eye DiseasesHelmholtz Moscow Research Institute of Eye DiseasesHelmholtz Moscow Research Institute of Eye DiseasesHelmholtz Moscow Research Institute of Eye DiseasesHelmholtz Moscow Research Institute of Eye DiseasesHelmholtz Moscow Research Institute of Eye DiseasesHelmholtz Moscow Research Institute of Eye DiseasesResults of comprehensive ELISA tests of blood serum for the presence of IgM-, IgA-, and IgG-antibodies to herpes simplex virus types 1 and 2, cytomegalovirus, Epstein-Barr virus, human herpesvirus 6, human herpes virus 8 type, Chlamydia trachomatis in 38 patients with uveal melanoma are presented. The polymerase chain reaction was used to detect DNA of these pathogens in tumor biopsies, vitreous body of 10 enucleated eyes, as well as in plasma IgG-antibodies to HHV 6 were revealed in 50% of patients; IgG-antibodies to HHV 8, in 5.3% of patients. Among the 16 patients with uveal melanoma at advanced stages, 6 patients had antibodies indicative of EBV reactivation (1.2-3.3). Chlamydia trachomatis genome was detected in both biopsies; in one of them, in conjunction with EBV and CMV DNA . Tissue samples from the identified infectious agents were related only to the spindle-cell histologic type AB of uveal melanoma. In plasma, genomes of pathogens were not determined. The results indicate the presence of infectious agents in patients with uveal melanoma and require further study of the pathogenetic role of infections in the pathogenesis of uveal melanoma.https://virusjour.crie.ru/jour/article/viewFile/109/40uveal melanomacytomegalovirusherpes simplex virus type 6 and 8epstein-barr viruschlamydia trachomatispolymerase chain reaction
spellingShingle S. V. Saakyan
E. B. Myakoshina
G. I. Krichevskaya
O. S. Slepova
O. G. Panteleeva
A. E. Andryushin
I. P. Khoroshilova
G. P. Zakharova
Testing patients with uveal melanoma for herpesvirus infections
Вопросы вирусологии
uveal melanoma
cytomegalovirus
herpes simplex virus type 6 and 8
epstein-barr virus
chlamydia trachomatis
polymerase chain reaction
title Testing patients with uveal melanoma for herpesvirus infections
title_full Testing patients with uveal melanoma for herpesvirus infections
title_fullStr Testing patients with uveal melanoma for herpesvirus infections
title_full_unstemmed Testing patients with uveal melanoma for herpesvirus infections
title_short Testing patients with uveal melanoma for herpesvirus infections
title_sort testing patients with uveal melanoma for herpesvirus infections
topic uveal melanoma
cytomegalovirus
herpes simplex virus type 6 and 8
epstein-barr virus
chlamydia trachomatis
polymerase chain reaction
url https://virusjour.crie.ru/jour/article/viewFile/109/40
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