Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients
Cleft lip and palate (CLP) is one of the craniofacial defects. The objective of this study was to identify the differences in appearance between the tissue factors in cartilage of CLP patients after primary and secondary rhinoplasty. Immunohistochemistry was performed with MMP-2, MMP-8, MMP-9, TIMP-...
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| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Pediatric Reports |
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| Online Access: | https://www.mdpi.com/2036-7503/14/4/50 |
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| author | Dace Buile Mara Pilmane Ilze Akota |
| author_facet | Dace Buile Mara Pilmane Ilze Akota |
| author_sort | Dace Buile |
| collection | DOAJ |
| description | Cleft lip and palate (CLP) is one of the craniofacial defects. The objective of this study was to identify the differences in appearance between the tissue factors in cartilage of CLP patients after primary and secondary rhinoplasty. Immunohistochemistry was performed with MMP-2, MMP-8, MMP-9, TIMP-2, IL-1α, IL-10, bFGF, and TGFβ1. The quantification of the structures was performed using a semi-quantitative census method. MMP-2, -9, IL-1a, and bFGF demonstrated higher number of positive cells in patients, while the number of MMP-8, IL-1a, -10 and TGFβ1 cells was higher or equal in the control subjects. The only statistically significant difference between CLP-operated patients was found in the TIMP-2 group, where the primary CLP patient group had a higher number of TIMP-2 positive chondrocytes than the secondary CLP patient group (U = 53.5; <i>p</i> = 0.021). The median value of the primary CLP group was ++ number of TIMP-2 positive chondrocytes compared to +++ in the secondary CLP group. No statistically significant difference was found between primary and secondary rhinoplasty patients for other tissue factors. Commonly, the rich expression of different tissue factors suggests a stimulation of higher elasticity in cleft affected cartilage. The statistically significant TIMP-2 elevation in primary operated cartilage indicates an impact of the selective tissue remodeling for hard tissue. |
| first_indexed | 2024-03-09T15:58:57Z |
| format | Article |
| id | doaj.art-02c785e8942644108169ed43178d8217 |
| institution | Directory Open Access Journal |
| issn | 2036-7503 |
| language | English |
| last_indexed | 2024-03-09T15:58:57Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pediatric Reports |
| spelling | doaj.art-02c785e8942644108169ed43178d82172023-11-24T17:15:02ZengMDPI AGPediatric Reports2036-75032022-10-0114441943310.3390/pediatric14040050Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate PatientsDace Buile0Mara Pilmane1Ilze Akota2Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradiņš University, 9 Kronvalda Str., LV-1010 Riga, LatviaDepartment of Morphology, Institute of Anatomy and Anthropology, Riga Stradiņš University, 9 Kronvalda Str., LV-1010 Riga, LatviaDepartment of Maxillofacial Surgery, Institute of Stomatology, Riga Stradiņš University, 20 Dzirciema Str., LV-1007 Riga, LatviaCleft lip and palate (CLP) is one of the craniofacial defects. The objective of this study was to identify the differences in appearance between the tissue factors in cartilage of CLP patients after primary and secondary rhinoplasty. Immunohistochemistry was performed with MMP-2, MMP-8, MMP-9, TIMP-2, IL-1α, IL-10, bFGF, and TGFβ1. The quantification of the structures was performed using a semi-quantitative census method. MMP-2, -9, IL-1a, and bFGF demonstrated higher number of positive cells in patients, while the number of MMP-8, IL-1a, -10 and TGFβ1 cells was higher or equal in the control subjects. The only statistically significant difference between CLP-operated patients was found in the TIMP-2 group, where the primary CLP patient group had a higher number of TIMP-2 positive chondrocytes than the secondary CLP patient group (U = 53.5; <i>p</i> = 0.021). The median value of the primary CLP group was ++ number of TIMP-2 positive chondrocytes compared to +++ in the secondary CLP group. No statistically significant difference was found between primary and secondary rhinoplasty patients for other tissue factors. Commonly, the rich expression of different tissue factors suggests a stimulation of higher elasticity in cleft affected cartilage. The statistically significant TIMP-2 elevation in primary operated cartilage indicates an impact of the selective tissue remodeling for hard tissue.https://www.mdpi.com/2036-7503/14/4/50CLPcartilagerhinoplastyimmunohistochemistry |
| spellingShingle | Dace Buile Mara Pilmane Ilze Akota Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients Pediatric Reports CLP cartilage rhinoplasty immunohistochemistry |
| title | Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients |
| title_full | Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients |
| title_fullStr | Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients |
| title_full_unstemmed | Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients |
| title_short | Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients |
| title_sort | evaluation of the multiple tissue factors in the cartilage of primary and secondary rhinoplasty in cleft lip and palate patients |
| topic | CLP cartilage rhinoplasty immunohistochemistry |
| url | https://www.mdpi.com/2036-7503/14/4/50 |
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