Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea
Objectives Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the pat...
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| Format: | Article |
| Language: | English |
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Korea Disease Control and Prevention Agency
2022-08-01
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| Series: | Osong Public Health and Research Perspectives |
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| Online Access: | http://ophrp.org/upload/pdf/j-phrp-2022-0232.pdf |
| _version_ | 1797710678121775104 |
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| author | Jin-Won Kim Minji Lee Hwachul Shin Chi-Hwan Choi Myung-Min Choi Jee Woong Kim Hwajung Yi Cheon-Kwon Yoo Gi-Eun Rhie |
| author_facet | Jin-Won Kim Minji Lee Hwachul Shin Chi-Hwan Choi Myung-Min Choi Jee Woong Kim Hwajung Yi Cheon-Kwon Yoo Gi-Eun Rhie |
| author_sort | Jin-Won Kim |
| collection | DOAJ |
| description | Objectives Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens. Methods Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products. Results Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing. Conclusion These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022. |
| first_indexed | 2024-03-12T06:54:41Z |
| format | Article |
| id | doaj.art-02c7b95491304277a805aaa8ff8fe782 |
| institution | Directory Open Access Journal |
| issn | 2210-9099 2210-9110 |
| language | English |
| last_indexed | 2024-03-12T06:54:41Z |
| publishDate | 2022-08-01 |
| publisher | Korea Disease Control and Prevention Agency |
| record_format | Article |
| series | Osong Public Health and Research Perspectives |
| spelling | doaj.art-02c7b95491304277a805aaa8ff8fe7822023-09-03T00:04:40ZengKorea Disease Control and Prevention AgencyOsong Public Health and Research Perspectives2210-90992210-91102022-08-0113430831110.24171/j.phrp.2022.0232677Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of KoreaJin-Won Kim0Minji Lee1Hwachul Shin2Chi-Hwan Choi3Myung-Min Choi4Jee Woong Kim5Hwajung Yi6Cheon-Kwon Yoo7Gi-Eun Rhie8 Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of Research Support, Department of Research Planning and Coordination, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, Korea Bureau of Infectious Diseases Diagnosis Control, Korea Disease Control and Prevention Agency, Cheongju, Korea Division of High-Risk Pathogens, Korea Disease Control and Prevention Agency, Cheongju, KoreaObjectives Monkeypox outbreaks in nonendemic countries have been reported since early May 2022. The first case of monkeypox in the Republic of Korea was confirmed in a patient who traveled to Europe in June 2022, and an attempt was made to isolate and identify the monkeypox virus (MPXV) from the patient’s specimens. Methods Clinical specimens from the patient were inoculated in Vero E6 cells. The isolated virus was identified as MPXV by the observation of cytopathic effects on Vero E6 cells, transmission electron microscopy, conventional polymerase chain reaction (PCR), and sequencing of PCR products. Results Cytopathic effects were observed in Vero E6 cells that were inoculated with skin lesion swab eluates. After multiple passages from the primary culture, orthopoxvirus morphology was observed using transmission electron microscopy. In addition, both MPXV-specific (F3L and ATI) and orthopoxvirus-specific genes (A39R, B2R, and HA) were confirmed by conventional PCR and Sanger sequencing. Conclusion These results indicate the successful isolation and identification of MPXV from the first patient in the Republic of Korea. The isolated virus was named MPXV-ROK-P1-2022.http://ophrp.org/upload/pdf/j-phrp-2022-0232.pdfidentificationisolationmonkeypox virus |
| spellingShingle | Jin-Won Kim Minji Lee Hwachul Shin Chi-Hwan Choi Myung-Min Choi Jee Woong Kim Hwajung Yi Cheon-Kwon Yoo Gi-Eun Rhie Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea Osong Public Health and Research Perspectives identification isolation monkeypox virus |
| title | Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea |
| title_full | Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea |
| title_fullStr | Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea |
| title_full_unstemmed | Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea |
| title_short | Isolation and identification of monkeypox virus MPXV-ROK-P1-2022 from the first case in the Republic of Korea |
| title_sort | isolation and identification of monkeypox virus mpxv rok p1 2022 from the first case in the republic of korea |
| topic | identification isolation monkeypox virus |
| url | http://ophrp.org/upload/pdf/j-phrp-2022-0232.pdf |
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