Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods

The aim of this study was to determine the nutrient and energy levels of red clover and lucerne forage. Investigation of forage at different maturity stages of three growths was carried out by chemical analysis, in vitro and in vivo digestibility methods. Generally, maturation caused a significant i...

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Main Authors: P. Homolka, V. Koukolová, M. Podsedníček, A. Hlaváčková
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2012-10-01
Series:Czech Journal of Animal Science
Subjects:
Online Access:https://cjas.agriculturejournals.cz/artkey/cjs-201210-0002_nutritive-value-of-red-clover-and-lucerne-forages-for-ruminants-estimated-by-in-vitro-and-in-vivo-digestibility.php
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author P. Homolka
V. Koukolová
M. Podsedníček
A. Hlaváčková
author_facet P. Homolka
V. Koukolová
M. Podsedníček
A. Hlaváčková
author_sort P. Homolka
collection DOAJ
description The aim of this study was to determine the nutrient and energy levels of red clover and lucerne forage. Investigation of forage at different maturity stages of three growths was carried out by chemical analysis, in vitro and in vivo digestibility methods. Generally, maturation caused a significant increase in fibre fractions. With the increasing maturity of forage samples the in vivo, in vitro, and calculated in vivo (in vivocalcul) digestibilities of organic matter (OM) linearly decreased. The in vitro and in vivocalcul digestibilities of OM averaged 0.754 and 0.708 for red clover and 0.717 and 0.667 for lucerne, respectively. The in vivo OM digestibility averaged 0.710 for red clover and 0.666 for lucerne. Gross energy (GE), digestible energy (DE), metabolizable energy (ME), net energy for lactation (NEL), and net energy for growth (NEG) averaged 18.12, 12.41, 9.60, 5.67, 5.50 and 18.09, 11.56, 9.01, 5.26, 4.99 MJ/kg of dry matter for red clover and lucerne, respectively. The effect of a vegetative stage on energy values of both forages was diverged for various growth times. When data were pooled across the estimated season, seven cutting-specific equations for descriptions of GE, DE, ME, NEL, NEG, in vitro, and in vivo OM digestibilities were obtained for red clover and lucerne, separately. The red clover model expression gave similar prediction equations for lucerne. It was possible to predict cutting-specific equations with coefficients of determination R2 > 0.719 for red clover and R2 > 0.400 for lucerne of the variation in GE, DE, ME, NEL, and NEG. The in vitro and in vivo OM digestibility equations were predicted with R2 being 0.840 (in vitro) and 0.707 (in vivo) for red clover, and 0.979 (in vitro) and 0.937 (in vivo) for lucerne. The parameters of these specific equations were statistically preferable than the general model expression which included both forages together.
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spelling doaj.art-02c8bacb9de24bf385eb28c3eef547bf2023-02-23T03:33:00ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092012-10-01571045446810.17221/6346-CJAScjs-201210-0002Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methodsP. Homolka0V. Koukolová1M. Podsedníček2A. Hlaváčková3Institute of Animal Science, Prague-Uhříněves, Czech RepublicInstitute of Animal Science, Prague-Uhříněves, Czech RepublicInstitute of Animal Science, Prague-Uhříněves, Czech RepublicFaculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicThe aim of this study was to determine the nutrient and energy levels of red clover and lucerne forage. Investigation of forage at different maturity stages of three growths was carried out by chemical analysis, in vitro and in vivo digestibility methods. Generally, maturation caused a significant increase in fibre fractions. With the increasing maturity of forage samples the in vivo, in vitro, and calculated in vivo (in vivocalcul) digestibilities of organic matter (OM) linearly decreased. The in vitro and in vivocalcul digestibilities of OM averaged 0.754 and 0.708 for red clover and 0.717 and 0.667 for lucerne, respectively. The in vivo OM digestibility averaged 0.710 for red clover and 0.666 for lucerne. Gross energy (GE), digestible energy (DE), metabolizable energy (ME), net energy for lactation (NEL), and net energy for growth (NEG) averaged 18.12, 12.41, 9.60, 5.67, 5.50 and 18.09, 11.56, 9.01, 5.26, 4.99 MJ/kg of dry matter for red clover and lucerne, respectively. The effect of a vegetative stage on energy values of both forages was diverged for various growth times. When data were pooled across the estimated season, seven cutting-specific equations for descriptions of GE, DE, ME, NEL, NEG, in vitro, and in vivo OM digestibilities were obtained for red clover and lucerne, separately. The red clover model expression gave similar prediction equations for lucerne. It was possible to predict cutting-specific equations with coefficients of determination R2 > 0.719 for red clover and R2 > 0.400 for lucerne of the variation in GE, DE, ME, NEL, and NEG. The in vitro and in vivo OM digestibility equations were predicted with R2 being 0.840 (in vitro) and 0.707 (in vivo) for red clover, and 0.979 (in vitro) and 0.937 (in vivo) for lucerne. The parameters of these specific equations were statistically preferable than the general model expression which included both forages together.https://cjas.agriculturejournals.cz/artkey/cjs-201210-0002_nutritive-value-of-red-clover-and-lucerne-forages-for-ruminants-estimated-by-in-vitro-and-in-vivo-digestibility.phpruminantforage qualityorganic matter digestibilityenergy value
spellingShingle P. Homolka
V. Koukolová
M. Podsedníček
A. Hlaváčková
Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods
Czech Journal of Animal Science
ruminant
forage quality
organic matter digestibility
energy value
title Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods
title_full Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods
title_fullStr Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods
title_full_unstemmed Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods
title_short Nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods
title_sort nutritive value of red clover and lucerne forages for ruminants estimated by in vitro and in vivo digestibility methods
topic ruminant
forage quality
organic matter digestibility
energy value
url https://cjas.agriculturejournals.cz/artkey/cjs-201210-0002_nutritive-value-of-red-clover-and-lucerne-forages-for-ruminants-estimated-by-in-vitro-and-in-vivo-digestibility.php
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AT vkoukolova nutritivevalueofredcloverandlucerneforagesforruminantsestimatedbyinvitroandinvivodigestibilitymethods
AT mpodsednicek nutritivevalueofredcloverandlucerneforagesforruminantsestimatedbyinvitroandinvivodigestibilitymethods
AT ahlavackova nutritivevalueofredcloverandlucerneforagesforruminantsestimatedbyinvitroandinvivodigestibilitymethods