Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011
We assessed drug susceptibilities of 125 avian influenza A(H5N1) viruses isolated from poultry in Vietnam during 2009–2011. Of 25 clade 1.1 viruses, all possessed a marker of resistance to M2 blockers amantadine and rimantadine; 24 were inhibited by neuraminidase inhibitors. One clade 1.1 virus cont...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2013-12-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/19/12/13-0705_article |
| _version_ | 1818284814491451392 |
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| author | Ha T. Nguyen Tung Nguyen Vasiliy P. Mishin Katrina Sleeman Amanda Balish Joyce Jones Adrian Creanga Henju Marjuki Timothy M. Uyeki Dang H. Nguyen Diep T. Nguyen Hoa T. Do Alexander I. Klimov Charles T. Davis Larisa V. Gubareva |
| author_facet | Ha T. Nguyen Tung Nguyen Vasiliy P. Mishin Katrina Sleeman Amanda Balish Joyce Jones Adrian Creanga Henju Marjuki Timothy M. Uyeki Dang H. Nguyen Diep T. Nguyen Hoa T. Do Alexander I. Klimov Charles T. Davis Larisa V. Gubareva |
| author_sort | Ha T. Nguyen |
| collection | DOAJ |
| description | We assessed drug susceptibilities of 125 avian influenza A(H5N1) viruses isolated from poultry in Vietnam during 2009–2011. Of 25 clade 1.1 viruses, all possessed a marker of resistance to M2 blockers amantadine and rimantadine; 24 were inhibited by neuraminidase inhibitors. One clade 1.1 virus contained the R430W neuraminidase gene and reduced inhibition by oseltamivir, zanamivir, and laninamivir 12-, 73-, and 29-fold, respectively. Three of 30 clade 2.3.4 viruses contained a I223T mutation and showed 7-fold reduced inhibition by oseltamivir. One of 70 clade 2.3.2.1 viruses had the H275Y marker of oseltamivir resistance and exhibited highly reduced inhibition by oseltamivir and peramivir; antiviral agents DAS181 and favipiravir inhibited H275Y mutant virus replication in MDCK-SIAT1 cells. Replicative fitness of the H275Y mutant virus was comparable to that of wildtype virus. These findings highlight the role of drug susceptibility monitoring of H5N1 subtype viruses circulating among birds to inform antiviral stockpiling decisions for pandemic preparedness. |
| first_indexed | 2024-12-13T00:58:47Z |
| format | Article |
| id | doaj.art-02c960cc0676449f80f5883300bf87a0 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-13T00:58:47Z |
| publishDate | 2013-12-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-02c960cc0676449f80f5883300bf87a02022-12-22T00:04:44ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592013-12-0119121963197110.3201/eid1912.130705Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011Ha T. NguyenTung NguyenVasiliy P. MishinKatrina SleemanAmanda BalishJoyce JonesAdrian CreangaHenju MarjukiTimothy M. UyekiDang H. NguyenDiep T. NguyenHoa T. DoAlexander I. KlimovCharles T. DavisLarisa V. GubarevaWe assessed drug susceptibilities of 125 avian influenza A(H5N1) viruses isolated from poultry in Vietnam during 2009–2011. Of 25 clade 1.1 viruses, all possessed a marker of resistance to M2 blockers amantadine and rimantadine; 24 were inhibited by neuraminidase inhibitors. One clade 1.1 virus contained the R430W neuraminidase gene and reduced inhibition by oseltamivir, zanamivir, and laninamivir 12-, 73-, and 29-fold, respectively. Three of 30 clade 2.3.4 viruses contained a I223T mutation and showed 7-fold reduced inhibition by oseltamivir. One of 70 clade 2.3.2.1 viruses had the H275Y marker of oseltamivir resistance and exhibited highly reduced inhibition by oseltamivir and peramivir; antiviral agents DAS181 and favipiravir inhibited H275Y mutant virus replication in MDCK-SIAT1 cells. Replicative fitness of the H275Y mutant virus was comparable to that of wildtype virus. These findings highlight the role of drug susceptibility monitoring of H5N1 subtype viruses circulating among birds to inform antiviral stockpiling decisions for pandemic preparedness.https://wwwnc.cdc.gov/eid/article/19/12/13-0705_articleInfluenzavirusesH5N1OseltamivirH275Yantiviral |
| spellingShingle | Ha T. Nguyen Tung Nguyen Vasiliy P. Mishin Katrina Sleeman Amanda Balish Joyce Jones Adrian Creanga Henju Marjuki Timothy M. Uyeki Dang H. Nguyen Diep T. Nguyen Hoa T. Do Alexander I. Klimov Charles T. Davis Larisa V. Gubareva Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011 Emerging Infectious Diseases Influenza viruses H5N1 Oseltamivir H275Y antiviral |
| title | Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011 |
| title_full | Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011 |
| title_fullStr | Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011 |
| title_full_unstemmed | Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011 |
| title_short | Antiviral Susceptibility of Highly Pathogenic Avian Influenza A(H5N1) Viruses Isolated from Poultry, Vietnam, 2009–2011 |
| title_sort | antiviral susceptibility of highly pathogenic avian influenza a h5n1 viruses isolated from poultry vietnam 2009 2011 |
| topic | Influenza viruses H5N1 Oseltamivir H275Y antiviral |
| url | https://wwwnc.cdc.gov/eid/article/19/12/13-0705_article |
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