Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages
Abstract Background Tuberculosis (TB) remains a significant global health concern and its diagnosis is challenging due to the limitations in the specificity and sensitivity of the current diagnostic tests. Exosomes are bioactive 30–100 nm vesicles produced by most cell types and are found in almost...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-05-01
|
| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-017-1205-9 |
| _version_ | 1818893532297953280 |
|---|---|
| author | Shamila D. Alipoor Esmaeil Mortaz Payam Tabarsi Parissa Farnia Mehdi Mirsaeidi Johan Garssen Masoud Movassaghi Ian M. Adcock |
| author_facet | Shamila D. Alipoor Esmaeil Mortaz Payam Tabarsi Parissa Farnia Mehdi Mirsaeidi Johan Garssen Masoud Movassaghi Ian M. Adcock |
| author_sort | Shamila D. Alipoor |
| collection | DOAJ |
| description | Abstract Background Tuberculosis (TB) remains a significant global health concern and its diagnosis is challenging due to the limitations in the specificity and sensitivity of the current diagnostic tests. Exosomes are bioactive 30–100 nm vesicles produced by most cell types and are found in almost all human body fluids. Exosomal microRNAs (miRNAs) can transfer biological information between cells and tissues and may act as potential biomarkers in many diseases. In this pilot study, we assessed the miRNA profile of exosomes released from human monocyte-derived macrophages upon infection with Mycobacterium bovis Bacillus Calmette–Guerin (BCG). Methods Human monocytes were obtained from the peripheral blood of three healthy subjects and driven to a monocyte-derived macrophage (MDM) phenotype using standard protocols. MDMs were infected with BCG or left uninfected as control. 72 h post-infection, exosomes were collected from the cell culture medium, RNA was isolated and RNA-seq performed. The raw reads were filtered to eliminate adaptor and primer sequences and the sequences were run against the mature human miRNA sequences available in miRBase. MicroRNAs were identified using an E value <0.01. miRNA network analysis was performed using the DIANA miRNA tool, miRDB and functional KEGG pathway analysis. Results Infection of MDMs with BCG leads to the release of several exosomal miRNAs. These included miR-1224, -1293, -425, -4467, -4732, -484, -5094, -6848-6849, -4488 and -96 all of which were predicted to target metabolism and energy production-related pathways. Conclusions This study provides evidence for the release of specific exosomal miRNAs from BCG-infected MDMs. These exosomal miRNAs reflect host-pathogen interaction and subversion of host metabolic processes following infection. |
| first_indexed | 2024-12-19T18:14:06Z |
| format | Article |
| id | doaj.art-02c9870baa2a447bbdf53506e97ad192 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-19T18:14:06Z |
| publishDate | 2017-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-02c9870baa2a447bbdf53506e97ad1922022-12-21T20:11:09ZengBMCJournal of Translational Medicine1479-58762017-05-011511910.1186/s12967-017-1205-9Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophagesShamila D. Alipoor0Esmaeil Mortaz1Payam Tabarsi2Parissa Farnia3Mehdi Mirsaeidi4Johan Garssen5Masoud Movassaghi6Ian M. Adcock7Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical SciencesClinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical SciencesClinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical SciencesMycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical SciencesDivision of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of MiamiDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht UniversityDepartment of Pathology and Laboratory Medicine, University of California, Los Angeles (UCLA)Airways Disease Section, National Heart & Lung Institute, Imperial College LondonAbstract Background Tuberculosis (TB) remains a significant global health concern and its diagnosis is challenging due to the limitations in the specificity and sensitivity of the current diagnostic tests. Exosomes are bioactive 30–100 nm vesicles produced by most cell types and are found in almost all human body fluids. Exosomal microRNAs (miRNAs) can transfer biological information between cells and tissues and may act as potential biomarkers in many diseases. In this pilot study, we assessed the miRNA profile of exosomes released from human monocyte-derived macrophages upon infection with Mycobacterium bovis Bacillus Calmette–Guerin (BCG). Methods Human monocytes were obtained from the peripheral blood of three healthy subjects and driven to a monocyte-derived macrophage (MDM) phenotype using standard protocols. MDMs were infected with BCG or left uninfected as control. 72 h post-infection, exosomes were collected from the cell culture medium, RNA was isolated and RNA-seq performed. The raw reads were filtered to eliminate adaptor and primer sequences and the sequences were run against the mature human miRNA sequences available in miRBase. MicroRNAs were identified using an E value <0.01. miRNA network analysis was performed using the DIANA miRNA tool, miRDB and functional KEGG pathway analysis. Results Infection of MDMs with BCG leads to the release of several exosomal miRNAs. These included miR-1224, -1293, -425, -4467, -4732, -484, -5094, -6848-6849, -4488 and -96 all of which were predicted to target metabolism and energy production-related pathways. Conclusions This study provides evidence for the release of specific exosomal miRNAs from BCG-infected MDMs. These exosomal miRNAs reflect host-pathogen interaction and subversion of host metabolic processes following infection.http://link.springer.com/article/10.1186/s12967-017-1205-9MycobacteriumExosomemiRNAMacrophageBiomarker |
| spellingShingle | Shamila D. Alipoor Esmaeil Mortaz Payam Tabarsi Parissa Farnia Mehdi Mirsaeidi Johan Garssen Masoud Movassaghi Ian M. Adcock Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages Journal of Translational Medicine Mycobacterium Exosome miRNA Macrophage Biomarker |
| title | Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages |
| title_full | Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages |
| title_fullStr | Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages |
| title_full_unstemmed | Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages |
| title_short | Bovis Bacillus Calmette–Guerin (BCG) infection induces exosomal miRNA release by human macrophages |
| title_sort | bovis bacillus calmette guerin bcg infection induces exosomal mirna release by human macrophages |
| topic | Mycobacterium Exosome miRNA Macrophage Biomarker |
| url | http://link.springer.com/article/10.1186/s12967-017-1205-9 |
| work_keys_str_mv | AT shamiladalipoor bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages AT esmaeilmortaz bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages AT payamtabarsi bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages AT parissafarnia bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages AT mehdimirsaeidi bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages AT johangarssen bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages AT masoudmovassaghi bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages AT ianmadcock bovisbacilluscalmetteguerinbcginfectioninducesexosomalmirnareleasebyhumanmacrophages |