Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cells
Introduction: Kaffir lime (Citrus hystrix DC.) is a medicinal plant found in Southeast Asia, both fruit and leaf have been used in Thai traditional medicine and food recipes. Objectives: Previous report demonstrated that partially purified fraction F9 of kaffir lime has anti-leukemic activity. Thi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Chaing Mai University
2017-01-01
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| Series: | Journal of Associated Medical Sciences |
| Subjects: | |
| Online Access: | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/73567 |
| _version_ | 1811303170768371712 |
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| author | Wipavee Utthawang Chadarat Ampasavate Siriporn Okonogi Methee Rungrojsakul Sawitree Chiampanichayakul Singkome Tima Songyot Anuchapreeda |
| author_facet | Wipavee Utthawang Chadarat Ampasavate Siriporn Okonogi Methee Rungrojsakul Sawitree Chiampanichayakul Singkome Tima Songyot Anuchapreeda |
| author_sort | Wipavee Utthawang |
| collection | DOAJ |
| description | Introduction: Kaffir lime (Citrus hystrix DC.) is a medicinal plant found in Southeast Asia, both fruit and leaf have been used in Thai traditional medicine and food recipes.
Objectives: Previous report demonstrated that partially purified fraction F9 of kaffir lime has anti-leukemic activity. This study aimed to investigate the anti-proliferative effects of low doses of F9 in Molt4 (human acute lymphoblastic leukemia) cell line. Effects on cell cycle progression as well as cell cycle regulatory proteins at the related phases after treatment were also studied.
Materials and methods: Cytotoxic effect of F9 was studied by MTT assay, and its effect on cell cycle progression in Molt4 cells was further examined by flow cytometry. The effect on regulatory proteins was determined by Western blot analysis.
Results: The F9 showed cytotoxic activity with an IC50 value of 24.5 μg/mL and low dose at the concentration of IC20 (2.5 μg/mL) of F9 induced cell death (sub-G1) as analyzed by flow cytometry. The F9 also increased p53 level while decreased cyclin A, cyclin B, and cdc2 (regulatory proteins in S and G2/M phases) protein expressions in a time- and dose-dependent manner.
Conclusion: The F9 from kaffir lime leaf at low doses induced cell death. Moreover, it also inhibited cell cycle regulatory proteins. This new knowledge will be further investigated to determine active compounds in the future.
Journal of Associated Medical Sciences 2017; 50(1): 27-37. Doi: 10.14456/jams.2017.3 |
| first_indexed | 2024-04-13T07:42:02Z |
| format | Article |
| id | doaj.art-02c9e2c91dac44438a9650ad8b24d2bb |
| institution | Directory Open Access Journal |
| issn | 2539-6056 2539-6056 |
| language | English |
| last_indexed | 2024-04-13T07:42:02Z |
| publishDate | 2017-01-01 |
| publisher | Chaing Mai University |
| record_format | Article |
| series | Journal of Associated Medical Sciences |
| spelling | doaj.art-02c9e2c91dac44438a9650ad8b24d2bb2022-12-22T02:55:50ZengChaing Mai UniversityJournal of Associated Medical Sciences2539-60562539-60562017-01-01501272773567Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cellsWipavee Utthawang0Chadarat Ampasavate1Siriporn Okonogi2Methee Rungrojsakul3Sawitree Chiampanichayakul4Singkome Tima5Songyot Anuchapreeda61 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 3 Alternative Medical College, Chandrakasem Rajabhat University, Bangkok, Thailand1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 3 Alternative Medical College, Chandrakasem Rajabhat University, Bangkok, Thailand1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 3 Alternative Medical College, Chandrakasem Rajabhat University, Bangkok, Thailand1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 3 Alternative Medical College, Chandrakasem Rajabhat University, Bangkok, Thailand1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 3 Alternative Medical College, Chandrakasem Rajabhat University, Bangkok, Thailand1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 3 Alternative Medical College, Chandrakasem Rajabhat University, Bangkok, Thailand1 Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand 2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, Thailand 3 Alternative Medical College, Chandrakasem Rajabhat University, Bangkok, ThailandIntroduction: Kaffir lime (Citrus hystrix DC.) is a medicinal plant found in Southeast Asia, both fruit and leaf have been used in Thai traditional medicine and food recipes. Objectives: Previous report demonstrated that partially purified fraction F9 of kaffir lime has anti-leukemic activity. This study aimed to investigate the anti-proliferative effects of low doses of F9 in Molt4 (human acute lymphoblastic leukemia) cell line. Effects on cell cycle progression as well as cell cycle regulatory proteins at the related phases after treatment were also studied. Materials and methods: Cytotoxic effect of F9 was studied by MTT assay, and its effect on cell cycle progression in Molt4 cells was further examined by flow cytometry. The effect on regulatory proteins was determined by Western blot analysis. Results: The F9 showed cytotoxic activity with an IC50 value of 24.5 μg/mL and low dose at the concentration of IC20 (2.5 μg/mL) of F9 induced cell death (sub-G1) as analyzed by flow cytometry. The F9 also increased p53 level while decreased cyclin A, cyclin B, and cdc2 (regulatory proteins in S and G2/M phases) protein expressions in a time- and dose-dependent manner. Conclusion: The F9 from kaffir lime leaf at low doses induced cell death. Moreover, it also inhibited cell cycle regulatory proteins. This new knowledge will be further investigated to determine active compounds in the future. Journal of Associated Medical Sciences 2017; 50(1): 27-37. Doi: 10.14456/jams.2017.3https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/73567Citrus hystrix DCkaffir lime leafcell cycleleukemiaMolt4 cells |
| spellingShingle | Wipavee Utthawang Chadarat Ampasavate Siriporn Okonogi Methee Rungrojsakul Sawitree Chiampanichayakul Singkome Tima Songyot Anuchapreeda Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cells Journal of Associated Medical Sciences Citrus hystrix DC kaffir lime leaf cell cycle leukemia Molt4 cells |
| title | Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cells |
| title_full | Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cells |
| title_fullStr | Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cells |
| title_full_unstemmed | Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cells |
| title_short | Low doses of partially purified fraction of kaffir lime (Citrus hystrix DC.) leaf extract induce cell death in Molt4 cells |
| title_sort | low doses of partially purified fraction of kaffir lime citrus hystrix dc leaf extract induce cell death in molt4 cells |
| topic | Citrus hystrix DC kaffir lime leaf cell cycle leukemia Molt4 cells |
| url | https://www.tci-thaijo.org/index.php/bulletinAMS/article/view/73567 |
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