Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in Hens
Salmonellosis remains a major medical and an unmet socioeconomic challenge. Worldwide, more than three million deaths per year are associated with <i>Salmonella enterica</i> serovar Enteritidis infections. Although commercially available vaccines for use in poultry exist, their efficacy...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/9/3/216 |
| _version_ | 1797415003056242688 |
|---|---|
| author | Javier Ochoa-Repáraz Eduard Sebastià Marta Sitjà Ibai Tamayo Juan Manuel Irache Carlos Gamazo |
| author_facet | Javier Ochoa-Repáraz Eduard Sebastià Marta Sitjà Ibai Tamayo Juan Manuel Irache Carlos Gamazo |
| author_sort | Javier Ochoa-Repáraz |
| collection | DOAJ |
| description | Salmonellosis remains a major medical and an unmet socioeconomic challenge. Worldwide, more than three million deaths per year are associated with <i>Salmonella enterica</i> serovar Enteritidis infections. Although commercially available vaccines for use in poultry exist, their efficacy is limited. We previously described a method for isolating a heat extract (HE) fraction of the cell surface of <i>S.</i> Enteritidis that contained major antigenic complexes immunogenic in hens naturally infected with the bacterium. One single dose of S. Enteritidis’ HE induced protection against lethal salmonellosis in mice. Furthermore, HE encapsulation in nanoparticles of the copolymer of methyl vinyl ether and maleic anhydride (PVM/MA), Gantrez AN, improved and prolonged the protection against the disease in mice. We formulated new preparations of Gantrez AN nanoparticles with HE <i>S.</i> Enteritidis and assessed their stability in drinking water and their efficacy in hens after experimental infection. The oral treatment of six-week-old hens with two doses of HE nanoparticles significantly reduced the <i>Salmonella</i> excretion in hens. Due to the effectiveness of the treatment in reducing bacterial excretion, we conclude that HE nanoencapsulation obtained from S. Enteritidis is a viable novel vaccination approach against salmonellosis in farms. |
| first_indexed | 2024-03-09T05:41:48Z |
| format | Article |
| id | doaj.art-02ca50f3fe3a4ee08d1de06b3b64c93f |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T05:41:48Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-02ca50f3fe3a4ee08d1de06b3b64c93f2023-12-03T12:23:57ZengMDPI AGVaccines2076-393X2021-03-019321610.3390/vaccines9030216Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in HensJavier Ochoa-Repáraz0Eduard Sebastià1Marta Sitjà2Ibai Tamayo3Juan Manuel Irache4Carlos Gamazo5Department of Microbiology and Parasitology, Universidad de Navarra, 31008 Pamplona, SpainHipra Scientific, S.L., 17170 Amer, SpainHipra Scientific, S.L., 17170 Amer, SpainDepartment of Microbiology and Parasitology, Universidad de Navarra, 31008 Pamplona, SpainDepartment of Chemistry and Pharmaceutical Technology, Universidad de Navarra, 31008 Pamplona, SpainDepartment of Microbiology and Parasitology, Universidad de Navarra, 31008 Pamplona, SpainSalmonellosis remains a major medical and an unmet socioeconomic challenge. Worldwide, more than three million deaths per year are associated with <i>Salmonella enterica</i> serovar Enteritidis infections. Although commercially available vaccines for use in poultry exist, their efficacy is limited. We previously described a method for isolating a heat extract (HE) fraction of the cell surface of <i>S.</i> Enteritidis that contained major antigenic complexes immunogenic in hens naturally infected with the bacterium. One single dose of S. Enteritidis’ HE induced protection against lethal salmonellosis in mice. Furthermore, HE encapsulation in nanoparticles of the copolymer of methyl vinyl ether and maleic anhydride (PVM/MA), Gantrez AN, improved and prolonged the protection against the disease in mice. We formulated new preparations of Gantrez AN nanoparticles with HE <i>S.</i> Enteritidis and assessed their stability in drinking water and their efficacy in hens after experimental infection. The oral treatment of six-week-old hens with two doses of HE nanoparticles significantly reduced the <i>Salmonella</i> excretion in hens. Due to the effectiveness of the treatment in reducing bacterial excretion, we conclude that HE nanoencapsulation obtained from S. Enteritidis is a viable novel vaccination approach against salmonellosis in farms.https://www.mdpi.com/2076-393X/9/3/216<i>Salmonella</i> enteritidisvaccinenanoparticleshens |
| spellingShingle | Javier Ochoa-Repáraz Eduard Sebastià Marta Sitjà Ibai Tamayo Juan Manuel Irache Carlos Gamazo Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in Hens Vaccines <i>Salmonella</i> enteritidis vaccine nanoparticles hens |
| title | Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in Hens |
| title_full | Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in Hens |
| title_fullStr | Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in Hens |
| title_full_unstemmed | Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in Hens |
| title_short | Protection Conferred by Drinking Water Administration of a Nanoparticle-Based Vaccine against <i>Salmonella</i> Enteritidis in Hens |
| title_sort | protection conferred by drinking water administration of a nanoparticle based vaccine against i salmonella i enteritidis in hens |
| topic | <i>Salmonella</i> enteritidis vaccine nanoparticles hens |
| url | https://www.mdpi.com/2076-393X/9/3/216 |
| work_keys_str_mv | AT javierochoareparaz protectionconferredbydrinkingwateradministrationofananoparticlebasedvaccineagainstisalmonellaienteritidisinhens AT eduardsebastia protectionconferredbydrinkingwateradministrationofananoparticlebasedvaccineagainstisalmonellaienteritidisinhens AT martasitja protectionconferredbydrinkingwateradministrationofananoparticlebasedvaccineagainstisalmonellaienteritidisinhens AT ibaitamayo protectionconferredbydrinkingwateradministrationofananoparticlebasedvaccineagainstisalmonellaienteritidisinhens AT juanmanuelirache protectionconferredbydrinkingwateradministrationofananoparticlebasedvaccineagainstisalmonellaienteritidisinhens AT carlosgamazo protectionconferredbydrinkingwateradministrationofananoparticlebasedvaccineagainstisalmonellaienteritidisinhens |