Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication
Human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS) are still a scientific challenge as to their in vivo eradication. Continuous variability of the HIV genome causes the present therapies to lose efficiency over the course of time and prevents the successful development...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2003-12-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2003-8-4-278-286.pdf |
| _version_ | 1797908860686565376 |
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| author | Yüksel YILDIZ John J. ROSSI |
| author_facet | Yüksel YILDIZ John J. ROSSI |
| author_sort | Yüksel YILDIZ |
| collection | DOAJ |
| description | Human immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS) are still a scientific challenge as to their in vivo eradication. Continuous variability of the HIV genome causes the present therapies to lose efficiency over the course of time and prevents the successful development of a vaccine. That’s why; investigation of new therapy methods has come to agenda. In this respect, since all genes are expressed through RNA intermediates and since HIV is a RNA virus, gene therapy employing ribozymes (RNA enzymes) has gained important consideration as a possible treatment for AIDS. Since HIV-1 gp120 and gp41 envelope glycoproteins which provide binding and fusion of HIV to target cells are encoded by HIV envelope (env) gene, one of the factors determining HIV infectivity is expression of the HIV env gene. By selectively decreasing of the HIV env mRNA expression with ribozymes, HIV infectivity can be inhibited. To effectively apply ribozymes as therapeutic agents, they have to be able to access and bind to target RNA. In this study, the most accessible sites for ribozyme targeting in a region of the HIV-1 env RNA encoding gp 120 and gp41 proteins has been investigated using antisense and RNAseH mapping in cell extracts prepared from HIV-1 infected CEM T-lymphocytes. |
| first_indexed | 2024-04-10T11:00:34Z |
| format | Article |
| id | doaj.art-02cca103ee754bd289af284e6ff9daf3 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1300-932X |
| language | English |
| last_indexed | 2024-04-10T11:00:34Z |
| publishDate | 2003-12-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-02cca103ee754bd289af284e6ff9daf32023-02-15T16:19:43ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1300-932X2003-12-0184278286Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral ReplicationYüksel YILDIZ0John J. ROSSI1Department of Physiology, Loma Linda University, Loma Linda, CA 92350Department of Molecular Biology, Beckman Research Institute of the City of Hope, Duarte, CA, USAHuman immunodeficiency virus (HIV) and acquired immunodeficiency syndrome (AIDS) are still a scientific challenge as to their in vivo eradication. Continuous variability of the HIV genome causes the present therapies to lose efficiency over the course of time and prevents the successful development of a vaccine. That’s why; investigation of new therapy methods has come to agenda. In this respect, since all genes are expressed through RNA intermediates and since HIV is a RNA virus, gene therapy employing ribozymes (RNA enzymes) has gained important consideration as a possible treatment for AIDS. Since HIV-1 gp120 and gp41 envelope glycoproteins which provide binding and fusion of HIV to target cells are encoded by HIV envelope (env) gene, one of the factors determining HIV infectivity is expression of the HIV env gene. By selectively decreasing of the HIV env mRNA expression with ribozymes, HIV infectivity can be inhibited. To effectively apply ribozymes as therapeutic agents, they have to be able to access and bind to target RNA. In this study, the most accessible sites for ribozyme targeting in a region of the HIV-1 env RNA encoding gp 120 and gp41 proteins has been investigated using antisense and RNAseH mapping in cell extracts prepared from HIV-1 infected CEM T-lymphocytes.http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2003-8-4-278-286.pdfHIVRNARibozymeAccessibility |
| spellingShingle | Yüksel YILDIZ John J. ROSSI Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi HIV RNA Ribozyme Accessibility |
| title | Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication |
| title_full | Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication |
| title_fullStr | Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication |
| title_full_unstemmed | Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication |
| title_short | Investigation of the Most Accessible Sites for Hammerhead Ribozymes Targeting HIV-1 env mRNA to Inhibit Viral Replication |
| title_sort | investigation of the most accessible sites for hammerhead ribozymes targeting hiv 1 env mrna to inhibit viral replication |
| topic | HIV RNA Ribozyme Accessibility |
| url | http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2003-8-4-278-286.pdf |
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