Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>)
Canine distemper is a contagious and severe systemic viral disease that affects domestic and wild carnivores worldwide. In this study, two adult female ferrets (<i>Mustela putorius furo</i>) were evaluated for cutaneous lesions. Scab, fur, and swab samples from the external auditory cana...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/6/375 |
| _version_ | 1797592333086097408 |
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| author | Annalisa Guercio Francesco Mira Santina Di Bella Francesca Gucciardi Antonio Lastra Giuseppa Purpari Calogero Castronovo Melissa Pennisi Vincenzo Di Marco Lo Presti Maria Rizzo Elisabetta Giudice |
| author_facet | Annalisa Guercio Francesco Mira Santina Di Bella Francesca Gucciardi Antonio Lastra Giuseppa Purpari Calogero Castronovo Melissa Pennisi Vincenzo Di Marco Lo Presti Maria Rizzo Elisabetta Giudice |
| author_sort | Annalisa Guercio |
| collection | DOAJ |
| description | Canine distemper is a contagious and severe systemic viral disease that affects domestic and wild carnivores worldwide. In this study, two adult female ferrets (<i>Mustela putorius furo</i>) were evaluated for cutaneous lesions. Scab, fur, and swab samples from the external auditory canal, cutaneous lesions, and scrapings were analyzed. Canine distemper virus (CDV)-positive samples underwent RT-PCR/RFLP with the restriction enzyme PsiI, and the hemagglutinin gene sequence was obtained. According to the restriction enzyme and sequence analyses, the viral strains were typed as CDV field strains that are included within the Europe lineage and distinct from those including vaccinal CDV strains. The sequence analysis showed the highest nucleotide identity rates in older Europe lineage CDV strains collected from dogs and a fox in Europe. This study is the first to report on CDV infection in ferrets in southern Italy and contributes to the current knowledge about natural CDV infection in this species. In conclusion, vaccination remains crucial for preventing the disease and counteracting cross-species infection. Molecular biology techniques can enable the monitoring of susceptible wild animals by ensuring the active surveillance of CDV spread. |
| first_indexed | 2024-03-11T01:50:37Z |
| format | Article |
| id | doaj.art-02cd56ec440d4d35817bc9df5bd14841 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T01:50:37Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-02cd56ec440d4d35817bc9df5bd148412023-11-18T13:00:00ZengMDPI AGVeterinary Sciences2306-73812023-05-0110637510.3390/vetsci10060375Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>)Annalisa Guercio0Francesco Mira1Santina Di Bella2Francesca Gucciardi3Antonio Lastra4Giuseppa Purpari5Calogero Castronovo6Melissa Pennisi7Vincenzo Di Marco Lo Presti8Maria Rizzo9Elisabetta Giudice10Istituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyDepartment of Veterinary Science, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, ItalyIstituto Zooprofilattico Sperimentale della Sicilia “A. Mirri”, Via G. Marinuzzi, 3, 90129 Palermo, ItalyDepartment of Veterinary Science, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, ItalyDepartment of Veterinary Science, University of Messina, Polo Universitario dell’Annunziata, 98168 Messina, ItalyCanine distemper is a contagious and severe systemic viral disease that affects domestic and wild carnivores worldwide. In this study, two adult female ferrets (<i>Mustela putorius furo</i>) were evaluated for cutaneous lesions. Scab, fur, and swab samples from the external auditory canal, cutaneous lesions, and scrapings were analyzed. Canine distemper virus (CDV)-positive samples underwent RT-PCR/RFLP with the restriction enzyme PsiI, and the hemagglutinin gene sequence was obtained. According to the restriction enzyme and sequence analyses, the viral strains were typed as CDV field strains that are included within the Europe lineage and distinct from those including vaccinal CDV strains. The sequence analysis showed the highest nucleotide identity rates in older Europe lineage CDV strains collected from dogs and a fox in Europe. This study is the first to report on CDV infection in ferrets in southern Italy and contributes to the current knowledge about natural CDV infection in this species. In conclusion, vaccination remains crucial for preventing the disease and counteracting cross-species infection. Molecular biology techniques can enable the monitoring of susceptible wild animals by ensuring the active surveillance of CDV spread.https://www.mdpi.com/2306-7381/10/6/375CDVferretcutaneous lesionsRT-PCRPCR-RFLPvaccine |
| spellingShingle | Annalisa Guercio Francesco Mira Santina Di Bella Francesca Gucciardi Antonio Lastra Giuseppa Purpari Calogero Castronovo Melissa Pennisi Vincenzo Di Marco Lo Presti Maria Rizzo Elisabetta Giudice Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>) Veterinary Sciences CDV ferret cutaneous lesions RT-PCR PCR-RFLP vaccine |
| title | Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>) |
| title_full | Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>) |
| title_fullStr | Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>) |
| title_full_unstemmed | Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>) |
| title_short | Biomolecular Analysis of Canine Distemper Virus Strains in Two Domestic Ferrets (<i>Mustela putorius furo</i>) |
| title_sort | biomolecular analysis of canine distemper virus strains in two domestic ferrets i mustela putorius furo i |
| topic | CDV ferret cutaneous lesions RT-PCR PCR-RFLP vaccine |
| url | https://www.mdpi.com/2306-7381/10/6/375 |
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