Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> Mice
Stevioside, the primary sweetener in stevia, is a glycoside with numerous beneficial biological activities. However, its anti-adipogenic effects on tissue differentiation and adipose tissues remain to be thoroughly investigated. In this study, the anti-adipogenic effects of stevioside during the dif...
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MDPI AG
2022-03-01
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| author | Miey Park Hana Baek Jin-Young Han Hae-Jeung Lee |
| author_facet | Miey Park Hana Baek Jin-Young Han Hae-Jeung Lee |
| author_sort | Miey Park |
| collection | DOAJ |
| description | Stevioside, the primary sweetener in stevia, is a glycoside with numerous beneficial biological activities. However, its anti-adipogenic effects on tissue differentiation and adipose tissues remain to be thoroughly investigated. In this study, the anti-adipogenic effects of stevioside during the differentiation of 3T3-L1 cells and epididymal adipose tissues of <i>db/db</i> mice were investigated by measuring the lipid droplets stained with Oil Red O and an immunoblot assay. Immunoblot analysis revealed that stevioside downregulated the expression of peroxisome proliferator-activated receptor-gamma (PPARγ), sterol regulatory element-binding protein-1c (SREBP-1c), CCAAT/enhancer-binding protein alpha (C/EBPα), and fatty acid synthase (FAS). Additionally, the protein expression of carnitine palmitoyltransferase 1 (CPT1), silent mating type information regulation 2 homolog 1 (SIRT1), and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) increased following treatment with stevioside. Furthermore, stevioside increased the phosphorylation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), both in vitro and in vivo. The activity of AMPK in stevioside-treated 3T3-L1 cells was further confirmed using agonists and antagonists of AMPK signaling. Our data indicate that stevioside ameliorates anti-adipogenic effects and promotes β-oxidation in adipocytes by activating AMPK-mediated signaling. The results of this study clearly demonstrated the inhibitory effect of stevioside on the differentiation of adipocytes and the reduction of lipid accumulation in the epididymal adipose tissues of <i>db/db</i> mice. |
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| last_indexed | 2024-03-09T12:01:17Z |
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| spelling | doaj.art-02d0120ad6af420abdd6f10cdc3c972b2023-11-30T23:03:10ZengMDPI AGCells2073-44092022-03-01117107610.3390/cells11071076Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> MiceMiey Park0Hana Baek1Jin-Young Han2Hae-Jeung Lee3Department of Food and Nutrition, Institute for Aging and Clinical Nutrition Research, College of BioNano Technology, Gachon University, Seongnam 13120, KoreaDepartment of Food and Nutrition, Institute for Aging and Clinical Nutrition Research, College of BioNano Technology, Gachon University, Seongnam 13120, KoreaDepartment of Food and Nutrition, Institute for Aging and Clinical Nutrition Research, College of BioNano Technology, Gachon University, Seongnam 13120, KoreaDepartment of Food and Nutrition, Institute for Aging and Clinical Nutrition Research, College of BioNano Technology, Gachon University, Seongnam 13120, KoreaStevioside, the primary sweetener in stevia, is a glycoside with numerous beneficial biological activities. However, its anti-adipogenic effects on tissue differentiation and adipose tissues remain to be thoroughly investigated. In this study, the anti-adipogenic effects of stevioside during the differentiation of 3T3-L1 cells and epididymal adipose tissues of <i>db/db</i> mice were investigated by measuring the lipid droplets stained with Oil Red O and an immunoblot assay. Immunoblot analysis revealed that stevioside downregulated the expression of peroxisome proliferator-activated receptor-gamma (PPARγ), sterol regulatory element-binding protein-1c (SREBP-1c), CCAAT/enhancer-binding protein alpha (C/EBPα), and fatty acid synthase (FAS). Additionally, the protein expression of carnitine palmitoyltransferase 1 (CPT1), silent mating type information regulation 2 homolog 1 (SIRT1), and peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) increased following treatment with stevioside. Furthermore, stevioside increased the phosphorylation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC), both in vitro and in vivo. The activity of AMPK in stevioside-treated 3T3-L1 cells was further confirmed using agonists and antagonists of AMPK signaling. Our data indicate that stevioside ameliorates anti-adipogenic effects and promotes β-oxidation in adipocytes by activating AMPK-mediated signaling. The results of this study clearly demonstrated the inhibitory effect of stevioside on the differentiation of adipocytes and the reduction of lipid accumulation in the epididymal adipose tissues of <i>db/db</i> mice.https://www.mdpi.com/2073-4409/11/7/1076steviosideadipogenesis3T3-L1 preadipocytesdifferentiation<i>db/db</i> mice |
| spellingShingle | Miey Park Hana Baek Jin-Young Han Hae-Jeung Lee Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> Mice Cells stevioside adipogenesis 3T3-L1 preadipocytes differentiation <i>db/db</i> mice |
| title | Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> Mice |
| title_full | Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> Mice |
| title_fullStr | Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> Mice |
| title_full_unstemmed | Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> Mice |
| title_short | Stevioside Enhances the Anti-Adipogenic Effect and β-Oxidation by Activating AMPK in 3T3-L1 Cells and Epididymal Adipose Tissues of <i>db/db</i> Mice |
| title_sort | stevioside enhances the anti adipogenic effect and β oxidation by activating ampk in 3t3 l1 cells and epididymal adipose tissues of i db db i mice |
| topic | stevioside adipogenesis 3T3-L1 preadipocytes differentiation <i>db/db</i> mice |
| url | https://www.mdpi.com/2073-4409/11/7/1076 |
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