High-Throughput and Accurate Determination of Transgene Copy Number and Zygosity in Transgenic Maize: From DNA Extraction to Data Analysis

High-Throughput and Accurate Determination of Transgene Copy Number and Zygosity in Transgenic Maize: From DNA Extraction to Data Analysis

It is vital to develop high-throughput methods to determine transgene copy numbers initially and zygosity during subsequent breeding. In this study, the target sequence of the previously reported endogenous reference gene <i>hmg</i> was analyzed using 633 maize inbred lines, and two SNPs...

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Bibliographic Details
Main Authors: Fang Liu, Jinkui Cheng, Xuhua Liu, Xi-Qing Wang
Format: Article
Language:English
Published: MDPI AG 2021-11-01
Series:International Journal of Molecular Sciences
Subjects:
high throughput
transgene copy number
zygosity
maize endogenous reference gene
single-nucleotide polymorphism
TaqMan assay
Online Access:https://www.mdpi.com/1422-0067/22/22/12487

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