Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts
In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC2...
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MDPI AG
2015-12-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/8/1/7 |
| _version_ | 1828149729934966784 |
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| author | Thao T. Nguyen Marie-Odile Parat Mark P. Hodson Jenny Pan Paul N. Shaw Amitha K. Hewavitharana |
| author_facet | Thao T. Nguyen Marie-Odile Parat Mark P. Hodson Jenny Pan Paul N. Shaw Amitha K. Hewavitharana |
| author_sort | Thao T. Nguyen |
| collection | DOAJ |
| description | In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer. |
| first_indexed | 2024-04-11T21:34:30Z |
| format | Article |
| id | doaj.art-02d3d528c464457e8bff75a7debc4b32 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T21:34:30Z |
| publishDate | 2015-12-01 |
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| series | Toxins |
| spelling | doaj.art-02d3d528c464457e8bff75a7debc4b322022-12-22T04:01:48ZengMDPI AGToxins2072-66512015-12-0181710.3390/toxins8010007toxins8010007Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf ExtractsThao T. Nguyen0Marie-Odile Parat1Mark P. Hodson2Jenny Pan3Paul N. Shaw4Amitha K. Hewavitharana5School of Pharmacy, The University of Queensland, Brisbane, QLD 4072, AustraliaSchool of Pharmacy, The University of Queensland, Brisbane, QLD 4072, AustraliaSchool of Pharmacy, The University of Queensland, Brisbane, QLD 4072, AustraliaSchool of Pharmacy, The University of Queensland, Brisbane, QLD 4072, AustraliaSchool of Pharmacy, The University of Queensland, Brisbane, QLD 4072, AustraliaSchool of Pharmacy, The University of Queensland, Brisbane, QLD 4072, AustraliaIn traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer.http://www.mdpi.com/2072-6651/8/1/7Carica papayacytotoxicitymass spectrometrycancerflavonoidschromatography |
| spellingShingle | Thao T. Nguyen Marie-Odile Parat Mark P. Hodson Jenny Pan Paul N. Shaw Amitha K. Hewavitharana Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts Toxins Carica papaya cytotoxicity mass spectrometry cancer flavonoids chromatography |
| title | Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts |
| title_full | Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts |
| title_fullStr | Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts |
| title_full_unstemmed | Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts |
| title_short | Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts |
| title_sort | chemical characterization and in vitro cytotoxicity on squamous cell carcinoma cells of carica papaya leaf extracts |
| topic | Carica papaya cytotoxicity mass spectrometry cancer flavonoids chromatography |
| url | http://www.mdpi.com/2072-6651/8/1/7 |
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