BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathway
Objective To explore the role of calcium release activated calcium channel protein 1 (Orai1) in bone marrow mesenchymal stem cells (BMSCs) osteogenesis induced by bone morphogenetic protein 9 (BMP9). Methods BMSCs were isolated by adherent method, and the third generation BMSCs were trea...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-10-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201257.pdf |
| _version_ | 1797365693524475904 |
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| author | ZHOU Jia-wang, LU Guo-hai, LI Xiao-lin |
| author_facet | ZHOU Jia-wang, LU Guo-hai, LI Xiao-lin |
| author_sort | ZHOU Jia-wang, LU Guo-hai, LI Xiao-lin |
| collection | DOAJ |
| description | Objective To explore the role of calcium release activated calcium channel protein 1 (Orai1) in bone marrow mesenchymal stem cells (BMSCs) osteogenesis induced by bone morphogenetic protein 9 (BMP9). Methods BMSCs were isolated by adherent method, and the third generation BMSCs were treated with 10－5mol/L of BMP9, and the mRNA expression of Orai1 was measured at different time points. RT-qPCR and Western blot were used to value the transfection efficiency after Orai1 knockdown. The osteogenic markers of Runx2, OPN and OCN were detected by RT-qPCR. Hydroxyapatite/PLA/chitosan, a scaffold material for bone tissue engineering, was implanted into the subcutaneous of nude mice. After 4 weeks, the osteogenic effect was examined by microscopy. Results The mRNA expression of Orai1 increased gradually at the 5th day after BMP9 induction (P＜0.05). The gene and protein expression of Orai1 decreased significantly after Orai1 knockdown (P＜0.05). Besides, the osteogenic effect of Orai1 knockdown decreased significantly, and the expression of Runx2, OPN and OCN gene decreased significantly (P＜0.05). Moreover, BMP9 could significantly improve the ectopic osteogenic ability of BMSCs, and Orai1 knockdown significantly inhibited this effect (P＜0.05). Conclusions BMP9 plays an important role in osteogenic differentiation of BMSCs potentially by regulating Orai1. |
| first_indexed | 2024-03-08T16:53:34Z |
| format | Article |
| id | doaj.art-02d4977e150445d2ae55d5f1df1f0682 |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:34Z |
| publishDate | 2021-10-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-02d4977e150445d2ae55d5f1df1f06822024-01-05T03:07:14ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-10-01411014701475BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathwayZHOU Jia-wang, LU Guo-hai, LI Xiao-lin01. Department of Orthopaedics, the Ninth People's Hospital of Suzhou, Wu Jiang Hospital Affiliated to Nantong University, Suzhou 215200;;2. Department of Orthopaedics, the North District of Suzhou Municipal Hospital, the Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou 215008, ChinaObjective To explore the role of calcium release activated calcium channel protein 1 (Orai1) in bone marrow mesenchymal stem cells (BMSCs) osteogenesis induced by bone morphogenetic protein 9 (BMP9). Methods BMSCs were isolated by adherent method, and the third generation BMSCs were treated with 10－5mol/L of BMP9, and the mRNA expression of Orai1 was measured at different time points. RT-qPCR and Western blot were used to value the transfection efficiency after Orai1 knockdown. The osteogenic markers of Runx2, OPN and OCN were detected by RT-qPCR. Hydroxyapatite/PLA/chitosan, a scaffold material for bone tissue engineering, was implanted into the subcutaneous of nude mice. After 4 weeks, the osteogenic effect was examined by microscopy. Results The mRNA expression of Orai1 increased gradually at the 5th day after BMP9 induction (P＜0.05). The gene and protein expression of Orai1 decreased significantly after Orai1 knockdown (P＜0.05). Besides, the osteogenic effect of Orai1 knockdown decreased significantly, and the expression of Runx2, OPN and OCN gene decreased significantly (P＜0.05). Moreover, BMP9 could significantly improve the ectopic osteogenic ability of BMSCs, and Orai1 knockdown significantly inhibited this effect (P＜0.05). Conclusions BMP9 plays an important role in osteogenic differentiation of BMSCs potentially by regulating Orai1.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201257.pdfbone morphogenetic protein 9 (bmp9)|orai1|bone marrow mesenchymal stem cells (bmscs)|osteogenic differentiation |
| spellingShingle | ZHOU Jia-wang, LU Guo-hai, LI Xiao-lin BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathway Jichu yixue yu linchuang bone morphogenetic protein 9 (bmp9)|orai1|bone marrow mesenchymal stem cells (bmscs)|osteogenic differentiation |
| title | BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathway |
| title_full | BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathway |
| title_fullStr | BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathway |
| title_full_unstemmed | BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathway |
| title_short | BMP9 promotes bone differentiation of bone marrow mesenchymal stem cells via Orai1 pathway |
| title_sort | bmp9 promotes bone differentiation of bone marrow mesenchymal stem cells via orai1 pathway |
| topic | bone morphogenetic protein 9 (bmp9)|orai1|bone marrow mesenchymal stem cells (bmscs)|osteogenic differentiation |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a201257.pdf |
| work_keys_str_mv | AT zhoujiawangluguohailixiaolin bmp9promotesbonedifferentiationofbonemarrowmesenchymalstemcellsviaorai1pathway |