Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cells

Background/purpose: Plasma rich in growth factors (PRGFs), which is prepared from autologous blood from patients, has been reported with regards to bone regeneration for dental implants. Human dental follicle cells (hDFCs) have the capacity to commit to multiple cell types such as the osteoblastic l...

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Main Authors: Hitoe Okada, Kosuke Takahashi, Naomi Ogura, Risa Tomoki, Ko Ito, Toshirou Kondoh
Format: Article
Language:English
Published: Elsevier 2016-09-01
Series:Journal of Dental Sciences
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1991790216000179
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author Hitoe Okada
Kosuke Takahashi
Naomi Ogura
Risa Tomoki
Ko Ito
Toshirou Kondoh
author_facet Hitoe Okada
Kosuke Takahashi
Naomi Ogura
Risa Tomoki
Ko Ito
Toshirou Kondoh
author_sort Hitoe Okada
collection DOAJ
description Background/purpose: Plasma rich in growth factors (PRGFs), which is prepared from autologous blood from patients, has been reported with regards to bone regeneration for dental implants. Human dental follicle cells (hDFCs) have the capacity to commit to multiple cell types such as the osteoblastic lineage. The aim of this study is to evaluate the effects of PRGFs for mineralization in hDFCs. Materials and methods: PRGFs was prepared from whole blood centrifuged at 460g for 8 minutes. hDFCs isolated from the dental follicle with collagenase/dispase were cultured with growth medium or osteogenic induction medium (OIM) containing PRGFs or fetal bovine serum. Concentrations of the growth factors were examined using an enzyme-linked immunosorbent assay kit. A cell migration assay was used for two-dimensional movement. Gene expressions were examined with real-time polymerase chain reaction using a DyNAmo SYBR Green quantitative polymerase chain reaction kit. Results: The platelet concentration in PRGF Fraction 2 was 2.14-fold higher than in whole blood. White blood cells were not detected in PRGFs. Transforming growth factor-β levels were higher than insulin-like growth factor-1, platelet-derived growth factor-AB and -BB, and vascular endothelial growth factors in PRGF Fraction 2. Proliferation and migration by hDFCs increased in OIM supplemented with PRGFs in a dose-dependent manner and were higher in hDFCs cultured in OIM plus 10% PRGFs compared with OIM plus 10% fetal bovine serum. PRGFs upregulated the gene expression of type I collagen, osteomodulin, alkaline phosphatase, bone morphogenic protein-4, and transforming growth factor-β in hDFCs. Conclusion: PRGFs may promote bone regeneration due to it including high levels of growth factors.
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spelling doaj.art-02d4a009345643d9aa2d0074c00e3ec52022-12-21T20:30:08ZengElsevierJournal of Dental Sciences1991-79022016-09-0111324525210.1016/j.jds.2015.12.001Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cellsHitoe Okada0Kosuke Takahashi1Naomi Ogura2Risa Tomoki3Ko Ito4Toshirou Kondoh5Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, JapanDepartment of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, JapanDepartment of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, JapanDepartment of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, JapanDepartment of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, JapanDepartment of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, JapanBackground/purpose: Plasma rich in growth factors (PRGFs), which is prepared from autologous blood from patients, has been reported with regards to bone regeneration for dental implants. Human dental follicle cells (hDFCs) have the capacity to commit to multiple cell types such as the osteoblastic lineage. The aim of this study is to evaluate the effects of PRGFs for mineralization in hDFCs. Materials and methods: PRGFs was prepared from whole blood centrifuged at 460g for 8 minutes. hDFCs isolated from the dental follicle with collagenase/dispase were cultured with growth medium or osteogenic induction medium (OIM) containing PRGFs or fetal bovine serum. Concentrations of the growth factors were examined using an enzyme-linked immunosorbent assay kit. A cell migration assay was used for two-dimensional movement. Gene expressions were examined with real-time polymerase chain reaction using a DyNAmo SYBR Green quantitative polymerase chain reaction kit. Results: The platelet concentration in PRGF Fraction 2 was 2.14-fold higher than in whole blood. White blood cells were not detected in PRGFs. Transforming growth factor-β levels were higher than insulin-like growth factor-1, platelet-derived growth factor-AB and -BB, and vascular endothelial growth factors in PRGF Fraction 2. Proliferation and migration by hDFCs increased in OIM supplemented with PRGFs in a dose-dependent manner and were higher in hDFCs cultured in OIM plus 10% PRGFs compared with OIM plus 10% fetal bovine serum. PRGFs upregulated the gene expression of type I collagen, osteomodulin, alkaline phosphatase, bone morphogenic protein-4, and transforming growth factor-β in hDFCs. Conclusion: PRGFs may promote bone regeneration due to it including high levels of growth factors.http://www.sciencedirect.com/science/article/pii/S1991790216000179PRGFgrowth factorsdental follicle cellsosteogenic differentiation
spellingShingle Hitoe Okada
Kosuke Takahashi
Naomi Ogura
Risa Tomoki
Ko Ito
Toshirou Kondoh
Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cells
Journal of Dental Sciences
PRGF
growth factors
dental follicle cells
osteogenic differentiation
title Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cells
title_full Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cells
title_fullStr Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cells
title_full_unstemmed Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cells
title_short Plasma rich in growth factors stimulates proliferation, migration, and gene expression associated with bone formation in human dental follicle cells
title_sort plasma rich in growth factors stimulates proliferation migration and gene expression associated with bone formation in human dental follicle cells
topic PRGF
growth factors
dental follicle cells
osteogenic differentiation
url http://www.sciencedirect.com/science/article/pii/S1991790216000179
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