Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways
Liquiritin is a natural flavone with a variety of pharmacological effects derived from the medicinal food homology plant Glycyrrhiza uralensis Fisch. As a kind of lethal allergic reactions, pseudo-allergic reactions (PARs) arise from the Mas-related G protein coupled receptor X2 (MRGPRX2)-triggered...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023004978 |
| _version_ | 1811161751892262912 |
|---|---|
| author | Lu Wang Chuyue Huang Zhili Li Guizhou Hu Jin Qi Zhimin Fan |
| author_facet | Lu Wang Chuyue Huang Zhili Li Guizhou Hu Jin Qi Zhimin Fan |
| author_sort | Lu Wang |
| collection | DOAJ |
| description | Liquiritin is a natural flavone with a variety of pharmacological effects derived from the medicinal food homology plant Glycyrrhiza uralensis Fisch. As a kind of lethal allergic reactions, pseudo-allergic reactions (PARs) arise from the Mas-related G protein coupled receptor X2 (MRGPRX2)-triggered fast degranulation of mast cells (MCs). In the current work, the anti-pseudo-allergy action and potential mechanisms of liquiritin were explored in vivo and in vitro. Liquiritin suppressed the calcium influx and degranulation elicited by Compound 48/80 (C48/80) in mouse peritoneal mast cells (MPMCs). In mice, liquiritin also inhibited the C48/80-elicited hind paw extravasation, as well as the elevations in TNF-α and histamine levels. Molecular docking combined with detection of HEK293T cells expressing human MRGPRX2 showed that liquiritin was a potential MRGPRX2 antagonist and inhibited PARs through the PI3K/AKT and PLCγ signaling pathways downstream of MRGPRX2. The present work opens a new avenue for the PARs management. |
| first_indexed | 2024-04-10T06:20:09Z |
| format | Article |
| id | doaj.art-02d8149efad84061a27d13ef3131699e |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T06:20:09Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-02d8149efad84061a27d13ef3131699e2023-03-02T05:00:51ZengElsevierHeliyon2405-84402023-02-0192e13290Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathwaysLu Wang0Chuyue Huang1Zhili Li2Guizhou Hu3Jin Qi4Zhimin Fan5Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, PR China; Corresponding author.Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, PR ChinaNanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, PR ChinaChina Pharmaceutical University, Nanjing, 211198, PR ChinaChina Pharmaceutical University, Nanjing, 211198, PR ChinaNanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210022, PR China; Corresponding author.Liquiritin is a natural flavone with a variety of pharmacological effects derived from the medicinal food homology plant Glycyrrhiza uralensis Fisch. As a kind of lethal allergic reactions, pseudo-allergic reactions (PARs) arise from the Mas-related G protein coupled receptor X2 (MRGPRX2)-triggered fast degranulation of mast cells (MCs). In the current work, the anti-pseudo-allergy action and potential mechanisms of liquiritin were explored in vivo and in vitro. Liquiritin suppressed the calcium influx and degranulation elicited by Compound 48/80 (C48/80) in mouse peritoneal mast cells (MPMCs). In mice, liquiritin also inhibited the C48/80-elicited hind paw extravasation, as well as the elevations in TNF-α and histamine levels. Molecular docking combined with detection of HEK293T cells expressing human MRGPRX2 showed that liquiritin was a potential MRGPRX2 antagonist and inhibited PARs through the PI3K/AKT and PLCγ signaling pathways downstream of MRGPRX2. The present work opens a new avenue for the PARs management.http://www.sciencedirect.com/science/article/pii/S2405844023004978LiquiritinAnti-Pseudo-allergyMAS-Related G protein-coupled receptor-X2PI3K/AKTPLCγImmunopharmacology |
| spellingShingle | Lu Wang Chuyue Huang Zhili Li Guizhou Hu Jin Qi Zhimin Fan Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways Heliyon Liquiritin Anti-Pseudo-allergy MAS-Related G protein-coupled receptor-X2 PI3K/AKT PLCγ Immunopharmacology |
| title | Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways |
| title_full | Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways |
| title_fullStr | Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways |
| title_full_unstemmed | Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways |
| title_short | Liquiritin inhibits MRGPRX2-mediated pseudo-allergy through the PI3K/AKT and PLCγ signaling pathways |
| title_sort | liquiritin inhibits mrgprx2 mediated pseudo allergy through the pi3k akt and plcγ signaling pathways |
| topic | Liquiritin Anti-Pseudo-allergy MAS-Related G protein-coupled receptor-X2 PI3K/AKT PLCγ Immunopharmacology |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023004978 |
| work_keys_str_mv | AT luwang liquiritininhibitsmrgprx2mediatedpseudoallergythroughthepi3kaktandplcgsignalingpathways AT chuyuehuang liquiritininhibitsmrgprx2mediatedpseudoallergythroughthepi3kaktandplcgsignalingpathways AT zhilili liquiritininhibitsmrgprx2mediatedpseudoallergythroughthepi3kaktandplcgsignalingpathways AT guizhouhu liquiritininhibitsmrgprx2mediatedpseudoallergythroughthepi3kaktandplcgsignalingpathways AT jinqi liquiritininhibitsmrgprx2mediatedpseudoallergythroughthepi3kaktandplcgsignalingpathways AT zhiminfan liquiritininhibitsmrgprx2mediatedpseudoallergythroughthepi3kaktandplcgsignalingpathways |