Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado
Citrullus lanatus (Thunb.) is an important crop in Brazil and affected by relevant insect vectors, including thrips (Thysanoptera: Thripidae). The present work was focused on evaluating the presence of Groundnut ringspot virus (GRSV) associated with Frankliniella sp. (Thysanoptera: Thripidae) in wat...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidad de Nariño
2023-08-01
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| Series: | Revista de Ciencias Agrícolas |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.22267/rcia |
| _version_ | 1797672259252387840 |
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| author | Ananias Queiroz Melissa Alexandra Romero Gil Santos Raimundo Wagner Aguiar |
| author_facet | Ananias Queiroz Melissa Alexandra Romero Gil Santos Raimundo Wagner Aguiar |
| author_sort | Ananias Queiroz |
| collection | DOAJ |
| description | Citrullus lanatus (Thunb.) is an important crop in Brazil and affected by relevant insect vectors, including thrips (Thysanoptera: Thripidae). The present work was focused on evaluating the presence of Groundnut ringspot virus (GRSV) associated with Frankliniella sp. (Thysanoptera: Thripidae) in watermelon, C. lanatus, cultivated in the Brazilian Cerrado biome. Several species of thrips were collected from four commercial crops located in Gurupi, Lagoa da Confusão, Formoso do Araguaia and Porto Nacional counties, all of them belonging to the Brazilian state of Tocantins). The total viral RNA obtained from the thrips was enriched and used to perform ugh RT-PCR. Using specific primers, a 644 bp fragment of the GRSV nucleocapsid was identified in all insect samples, which allowed us to perform a phylogenetic analysis with GRSV sequences described for other geographical regions. The findings demonstrate the wide distribution of the GRSV virus in commercial watermelon crops in the Brazilian Cerrado Biome. The association with Frankliniella sp. is highlighted as one of the most relevant vector pathways in this region of high intensity and advanced agriculture. In addition, phylogenetic analysis demonstrated a close relationship between GRSV sequences from South Africa and Brazil. These findings may contribute to the understanding of GRSV distribution in watermelon crops in different localities of Brazilian Cerrado Biome. |
| first_indexed | 2024-03-11T21:27:27Z |
| format | Article |
| id | doaj.art-02d9218bf10746d7b7772401f5b341c4 |
| institution | Directory Open Access Journal |
| issn | 0120-0135 2256-2273 |
| language | English |
| last_indexed | 2024-03-11T21:27:27Z |
| publishDate | 2023-08-01 |
| publisher | Universidad de Nariño |
| record_format | Article |
| series | Revista de Ciencias Agrícolas |
| spelling | doaj.art-02d9218bf10746d7b7772401f5b341c42023-09-27T14:28:42ZengUniversidad de NariñoRevista de Ciencias Agrícolas0120-01352256-22732023-08-0140210.22267/rcia.20234002.211Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian CerradoAnanias Queiroz0https://orcid.org/0000-0002-9626-5382Melissa Alexandra Romero1https://orcid.org/0000-0002-3601-6034Gil Santos2https://orcid.org/0000-0002-3830-9463Raimundo Wagner Aguiar3https://orcid.org/0000-0002-5169-4968Federal University of TocantinsFederal University of TocantinsFederal University of TocantinsUniversidade Federal do TocantinsCitrullus lanatus (Thunb.) is an important crop in Brazil and affected by relevant insect vectors, including thrips (Thysanoptera: Thripidae). The present work was focused on evaluating the presence of Groundnut ringspot virus (GRSV) associated with Frankliniella sp. (Thysanoptera: Thripidae) in watermelon, C. lanatus, cultivated in the Brazilian Cerrado biome. Several species of thrips were collected from four commercial crops located in Gurupi, Lagoa da Confusão, Formoso do Araguaia and Porto Nacional counties, all of them belonging to the Brazilian state of Tocantins). The total viral RNA obtained from the thrips was enriched and used to perform ugh RT-PCR. Using specific primers, a 644 bp fragment of the GRSV nucleocapsid was identified in all insect samples, which allowed us to perform a phylogenetic analysis with GRSV sequences described for other geographical regions. The findings demonstrate the wide distribution of the GRSV virus in commercial watermelon crops in the Brazilian Cerrado Biome. The association with Frankliniella sp. is highlighted as one of the most relevant vector pathways in this region of high intensity and advanced agriculture. In addition, phylogenetic analysis demonstrated a close relationship between GRSV sequences from South Africa and Brazil. These findings may contribute to the understanding of GRSV distribution in watermelon crops in different localities of Brazilian Cerrado Biome.http://dx.doi.org/10.22267/rciainsect vectororthotospovirusrt-pcrthysanopteraviral rnafrankliniella spwatermelonbraziliancitrullus lanatus |
| spellingShingle | Ananias Queiroz Melissa Alexandra Romero Gil Santos Raimundo Wagner Aguiar Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado Revista de Ciencias Agrícolas insect vector orthotospovirus rt-pcr thysanoptera viral rna frankliniella sp watermelon brazilian citrullus lanatus |
| title | Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado |
| title_full | Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado |
| title_fullStr | Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado |
| title_full_unstemmed | Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado |
| title_short | Detection of Groundnut ringspot virus carried by Frankliniella sp. on watermelon fields in Brazilian Cerrado |
| title_sort | detection of groundnut ringspot virus carried by frankliniella sp on watermelon fields in brazilian cerrado |
| topic | insect vector orthotospovirus rt-pcr thysanoptera viral rna frankliniella sp watermelon brazilian citrullus lanatus |
| url | http://dx.doi.org/10.22267/rcia |
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