Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS
<i>Zanthoxylum nitidum</i> (Roxb.) DC (Rutaceae), called as “liangmianzhen„ in China, is well known for its anti-inflammation and analgesic effect. Alkaloids are its main active constituents. However, little has been known about the absorption of main alkaloids in viv...
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| Format: | Article |
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MDPI AG
2019-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/3/585 |
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| author | Aihua Huang Yuguang Chi Jiawei Liu Mincun Wang Jialiang Qin Lijuan Ou Weiwen Chen Zhongxiang Zhao Ruoting Zhan Hui Xu |
| author_facet | Aihua Huang Yuguang Chi Jiawei Liu Mincun Wang Jialiang Qin Lijuan Ou Weiwen Chen Zhongxiang Zhao Ruoting Zhan Hui Xu |
| author_sort | Aihua Huang |
| collection | DOAJ |
| description | <i>Zanthoxylum nitidum</i> (Roxb.) DC (Rutaceae), called as “liangmianzhen„ in China, is well known for its anti-inflammation and analgesic effect. Alkaloids are its main active constituents. However, little has been known about the absorption of main alkaloids in vivo. In this study, an ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry was employed for identification of absorbed alkaloids in rats after oral administration of <i>Z. nitidum</i> decoction. By analyzing the fragmentation patterns, a total of nineteen alkaloids were exactly or tentatively identified in rat plasma after treatment, of which magnoflorine, α-allocryptopine, and skimmianine are dominant. Moreover, a high performance liquid chromatography coupled mass spectrometry method was developed for simultaneous quantification of magnoflorine, α-allocryptopine, and skimmianine, and successfully applied to pharmacokinetic study in rats after oral administration of <i>Z. nitidum</i> decoction. The research would contribute to comprehensive understanding of the material basis and function mechanism of <i>Z. nitidum</i> decoction. |
| first_indexed | 2024-12-11T10:12:52Z |
| format | Article |
| id | doaj.art-02e2ee71fd394cf88ada0859958e380b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-11T10:12:52Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-02e2ee71fd394cf88ada0859958e380b2022-12-22T01:11:42ZengMDPI AGMolecules1420-30492019-02-0124358510.3390/molecules24030585molecules24030585Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MSAihua Huang0Yuguang Chi1Jiawei Liu2Mincun Wang3Jialiang Qin4Lijuan Ou5Weiwen Chen6Zhongxiang Zhao7Ruoting Zhan8Hui Xu9Key Laboratory of Ministry of Education, Research Center of Chinese Herbal Resources and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaKey Laboratory of Ministry of Education, Research Center of Chinese Herbal Resources and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaKey Laboratory of Ministry of Education, Research Center of Chinese Herbal Resources and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaKey Laboratory of Ministry of Education, Research Center of Chinese Herbal Resources and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaKey Laboratory of Ministry of Education, Research Center of Chinese Herbal Resources and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, ChinaKey Laboratory of Ministry of Education, Research Center of Chinese Herbal Resources and Engineering, Guangzhou University of Chinese Medicine, Guangzhou 510006, China<i>Zanthoxylum nitidum</i> (Roxb.) DC (Rutaceae), called as “liangmianzhen„ in China, is well known for its anti-inflammation and analgesic effect. Alkaloids are its main active constituents. However, little has been known about the absorption of main alkaloids in vivo. In this study, an ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry was employed for identification of absorbed alkaloids in rats after oral administration of <i>Z. nitidum</i> decoction. By analyzing the fragmentation patterns, a total of nineteen alkaloids were exactly or tentatively identified in rat plasma after treatment, of which magnoflorine, α-allocryptopine, and skimmianine are dominant. Moreover, a high performance liquid chromatography coupled mass spectrometry method was developed for simultaneous quantification of magnoflorine, α-allocryptopine, and skimmianine, and successfully applied to pharmacokinetic study in rats after oral administration of <i>Z. nitidum</i> decoction. The research would contribute to comprehensive understanding of the material basis and function mechanism of <i>Z. nitidum</i> decoction.https://www.mdpi.com/1420-3049/24/3/585<i>Zanthoxylum nitidum</i>UPLC-Q-TOF-MS/MSpharmacokinetic studyHPLC-MS/MSmagnoflorine |
| spellingShingle | Aihua Huang Yuguang Chi Jiawei Liu Mincun Wang Jialiang Qin Lijuan Ou Weiwen Chen Zhongxiang Zhao Ruoting Zhan Hui Xu Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS Molecules <i>Zanthoxylum nitidum</i> UPLC-Q-TOF-MS/MS pharmacokinetic study HPLC-MS/MS magnoflorine |
| title | Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS |
| title_full | Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS |
| title_fullStr | Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS |
| title_full_unstemmed | Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS |
| title_short | Profiling and Pharmacokinetic Studies of Alkaloids in Rats After Oral Administration of <i>Zanthoxylum nitidum</i> Decoction by UPLC-Q-TOF-MS/MS and HPLC-MS/MS |
| title_sort | profiling and pharmacokinetic studies of alkaloids in rats after oral administration of i zanthoxylum nitidum i decoction by uplc q tof ms ms and hplc ms ms |
| topic | <i>Zanthoxylum nitidum</i> UPLC-Q-TOF-MS/MS pharmacokinetic study HPLC-MS/MS magnoflorine |
| url | https://www.mdpi.com/1420-3049/24/3/585 |
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