Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching Hospital
Polymyxin-resistant <i>Pseudomonas aeruginosa</i> is a major threat to public health globally. We investigated the prevalence of polymyxin-resistant <i>P. aeruginosa</i> in a Chinese teaching hospital and determined the genetic and drug-resistant phenotypes of the resistant i...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/6/799 |
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| author | Chenlu Xiao Yan Zhu Zhitao Yang Dake Shi Yuxing Ni Li Hua Jian Li |
| author_facet | Chenlu Xiao Yan Zhu Zhitao Yang Dake Shi Yuxing Ni Li Hua Jian Li |
| author_sort | Chenlu Xiao |
| collection | DOAJ |
| description | Polymyxin-resistant <i>Pseudomonas aeruginosa</i> is a major threat to public health globally. We investigated the prevalence of polymyxin-resistant <i>P. aeruginosa</i> in a Chinese teaching hospital and determined the genetic and drug-resistant phenotypes of the resistant isolates. <i>P. aeruginosa</i> isolates identified by MALDI-TOF MS were collected across a 3-month period in Ruijin Hospital. Antimicrobial susceptibility was determined by a Vitek-2 Compact system with broth dilution used to determine polymyxin B (PMB) susceptibility. Polymyxin-resistant isolates were further characterized by molecular typing using PCR, multi-locus sequence typing (MLST) and whole-genome sequencing. Phylogenetic relationships were analyzed using single nucleotide polymorphism (SNP) from the whole-genome sequencing. Of 362 <i>P. aeruginosa</i> isolates collected, 8 (2.2%) isolates from separate patients across six wards were polymyxin-resistant (MIC range, PMB 4–16 μg/mL and colistin 4–≥16 μg/mL). Four patients received PMB treatments (intravenous, aerosolized and/or topical) and all patients survived to discharge. All polymyxin-resistant isolates were genetically related and were assigned to five different clades (Isolate 150 and Isolate 211 being the same ST823 type). Genetic variations V51I, Y345H, G68S and R155H in <i>pmrB</i> and L71R in <i>pmrA</i> were identified, which might confer polymyxin resistance in these isolates. Six of the polymyxin-resistant isolates showed reduced susceptibility to imipenem and meropenem (MIC range ≥ 16 μg/mL), while two of the eight isolates were resistant to ceftazidime. We revealed a low prevalence of polymyxin-resistant <i>P. aeruginosa</i> in a Chinese teaching hospital with most polymyxin-resistant isolates being multidrug-resistant. Therefore, effective infection control measures are urgently needed to prevent further spread of resistance to the last-line polymyxins. |
| first_indexed | 2024-03-10T00:35:01Z |
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| id | doaj.art-02e378beaf1c4d4680841ba7f4da70ff |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T00:35:01Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-02e378beaf1c4d4680841ba7f4da70ff2023-11-23T15:17:32ZengMDPI AGAntibiotics2079-63822022-06-0111679910.3390/antibiotics11060799Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching HospitalChenlu Xiao0Yan Zhu1Zhitao Yang2Dake Shi3Yuxing Ni4Li Hua5Jian Li6Department of Laboratory Medicine, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaMonash Biomedicine Discovery Institute, Infection Program and Department of Microbiology, Monash University, Melbourne 3800, AustraliaDepartment of Emergency, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Infection Control, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaDepartment of Infection Control, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaSchool of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, ChinaMonash Biomedicine Discovery Institute, Infection Program and Department of Microbiology, Monash University, Melbourne 3800, AustraliaPolymyxin-resistant <i>Pseudomonas aeruginosa</i> is a major threat to public health globally. We investigated the prevalence of polymyxin-resistant <i>P. aeruginosa</i> in a Chinese teaching hospital and determined the genetic and drug-resistant phenotypes of the resistant isolates. <i>P. aeruginosa</i> isolates identified by MALDI-TOF MS were collected across a 3-month period in Ruijin Hospital. Antimicrobial susceptibility was determined by a Vitek-2 Compact system with broth dilution used to determine polymyxin B (PMB) susceptibility. Polymyxin-resistant isolates were further characterized by molecular typing using PCR, multi-locus sequence typing (MLST) and whole-genome sequencing. Phylogenetic relationships were analyzed using single nucleotide polymorphism (SNP) from the whole-genome sequencing. Of 362 <i>P. aeruginosa</i> isolates collected, 8 (2.2%) isolates from separate patients across six wards were polymyxin-resistant (MIC range, PMB 4–16 μg/mL and colistin 4–≥16 μg/mL). Four patients received PMB treatments (intravenous, aerosolized and/or topical) and all patients survived to discharge. All polymyxin-resistant isolates were genetically related and were assigned to five different clades (Isolate 150 and Isolate 211 being the same ST823 type). Genetic variations V51I, Y345H, G68S and R155H in <i>pmrB</i> and L71R in <i>pmrA</i> were identified, which might confer polymyxin resistance in these isolates. Six of the polymyxin-resistant isolates showed reduced susceptibility to imipenem and meropenem (MIC range ≥ 16 μg/mL), while two of the eight isolates were resistant to ceftazidime. We revealed a low prevalence of polymyxin-resistant <i>P. aeruginosa</i> in a Chinese teaching hospital with most polymyxin-resistant isolates being multidrug-resistant. Therefore, effective infection control measures are urgently needed to prevent further spread of resistance to the last-line polymyxins.https://www.mdpi.com/2079-6382/11/6/799<i>Pseudomonas aeruginosa</i>polymyxin resistancephenotypesgenomics |
| spellingShingle | Chenlu Xiao Yan Zhu Zhitao Yang Dake Shi Yuxing Ni Li Hua Jian Li Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching Hospital Antibiotics <i>Pseudomonas aeruginosa</i> polymyxin resistance phenotypes genomics |
| title | Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching Hospital |
| title_full | Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching Hospital |
| title_fullStr | Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching Hospital |
| title_full_unstemmed | Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching Hospital |
| title_short | Prevalence and Molecular Characteristics of Polymyxin-Resistant <i>Pseudomonas aeruginosa</i> in a Chinese Tertiary Teaching Hospital |
| title_sort | prevalence and molecular characteristics of polymyxin resistant i pseudomonas aeruginosa i in a chinese tertiary teaching hospital |
| topic | <i>Pseudomonas aeruginosa</i> polymyxin resistance phenotypes genomics |
| url | https://www.mdpi.com/2079-6382/11/6/799 |
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