EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYING
In this work maltodextrins were added to commercial GOS in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of L. plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00584/full |
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| author | Natalia eSosa Esteban eGerbino Marina Alejandra Golowczyc Carolina eSchebor Andrea eGomez-Zavaglia E. Elizabeth Tymczyszyn |
| author_facet | Natalia eSosa Esteban eGerbino Marina Alejandra Golowczyc Carolina eSchebor Andrea eGomez-Zavaglia E. Elizabeth Tymczyszyn |
| author_sort | Natalia eSosa |
| collection | DOAJ |
| description | In this work maltodextrins were added to commercial GOS in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of L. plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20ºC for 12 weeks, or at 30ºC for 6 weeks.The Tgs of GOS:MD matrices were about 20-30oC higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33 and 44% RH at 5, 20 and 30ºC.Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no MD were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5oC. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for each storage condition (k values). This information can be useful to calculate the shelf-life of spray-dried starters stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-drying stabilization of a potentially probiotic strain in the presence of GOS. |
| first_indexed | 2024-12-11T06:20:52Z |
| format | Article |
| id | doaj.art-02e4ec71db6e4aee88610b200dd7cec8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T06:20:52Z |
| publishDate | 2016-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-02e4ec71db6e4aee88610b200dd7cec82022-12-22T01:17:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-05-01710.3389/fmicb.2016.00584190361EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYINGNatalia eSosa0Esteban eGerbino1Marina Alejandra Golowczyc2Carolina eSchebor3Andrea eGomez-Zavaglia4E. Elizabeth Tymczyszyn5Facultad de Bromatologia. Universidad Nacional de Entre RiosCenter for Research and Development in Food Cryotechnology (CIDCA CCT Conicet)Center for Research and Development in Food Cryotechnology (CIDCA CCT Conicet)Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires.Center for Research and Development in Food Cryotechnology (CIDCA CCT Conicet)Universidad Nacional de QuilmesIn this work maltodextrins were added to commercial GOS in a 1:1 ratio and their thermophysical characteristics were analyzed. GOS:MD solutions were then used as matrices during spray-drying of L. plantarum CIDCA 83114. The obtained powders were equilibrated at different relative humidities (RH) and stored at 5 and 20ºC for 12 weeks, or at 30ºC for 6 weeks.The Tgs of GOS:MD matrices were about 20-30oC higher than those of GOS at RH within 11 and 52%. A linear relation between the spin-spin relaxation time (T2) and T-Tg parameter was observed for GOS:MD matrices equilibrated at 11, 22, 33 and 44% RH at 5, 20 and 30ºC.Spray-drying of L. plantarum CIDCA 83114 in GOS:MD matrices allowed the recovery of 93% microorganisms. In contrast, only 64% microorganisms were recovered when no MD were included in the dehydration medium. Survival of L. plantarum CIDCA 83114 during storage showed the best performance for bacteria stored at 5oC. In a further step, the slopes of the linear regressions provided information about the rate of microbial inactivation for each storage condition (k values). This information can be useful to calculate the shelf-life of spray-dried starters stored at different temperatures and RH. Using GOS:MD matrices as a dehydration medium enhanced the recovery of L. plantarum CIDCA 83114 after spray-drying. This strategy allowed for the first time the spray-drying stabilization of a potentially probiotic strain in the presence of GOS.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00584/fullLactic acid bacteriagalacto-oligosaccharidesglass transition temperatureMaltodextrinSpray-drying |
| spellingShingle | Natalia eSosa Esteban eGerbino Marina Alejandra Golowczyc Carolina eSchebor Andrea eGomez-Zavaglia E. Elizabeth Tymczyszyn EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYING Frontiers in Microbiology Lactic acid bacteria galacto-oligosaccharides glass transition temperature Maltodextrin Spray-drying |
| title | EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYING |
| title_full | EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYING |
| title_fullStr | EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYING |
| title_full_unstemmed | EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYING |
| title_short | EFFECT OF GALACTO-OLIGOSACCHARIDES:MALTODEXTRIN MATRICES ON THE RECOVERY OF LACTOBACILLUS PLANTARUM AFTER SPRAY-DRYING |
| title_sort | effect of galacto oligosaccharides maltodextrin matrices on the recovery of lactobacillus plantarum after spray drying |
| topic | Lactic acid bacteria galacto-oligosaccharides glass transition temperature Maltodextrin Spray-drying |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00584/full |
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