Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium
Chagas' myocardiopathy, caused by the intracellular protozoan Trypanosoma cruzi, is characterized by microvascular alterations, heart failure and arrhythmias. Ischemia and arrythmogenesis have been attributed to proteins shed by the parasite, although this has not been fully dem...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2013-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100058&lng=en&tlng=en |
| _version_ | 1811343120517824512 |
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| author | H. Rodriguez-Angulo J. Toro-Mendoza J. Marques R. Bonfante-Cabarcas A. Mijares |
| author_facet | H. Rodriguez-Angulo J. Toro-Mendoza J. Marques R. Bonfante-Cabarcas A. Mijares |
| author_sort | H. Rodriguez-Angulo |
| collection | DOAJ |
| description | Chagas' myocardiopathy, caused by the intracellular protozoan Trypanosoma cruzi, is characterized by microvascular alterations, heart failure and arrhythmias. Ischemia and arrythmogenesis have been attributed to proteins shed by the parasite, although this has not been fully demonstrated. The aim of the present investigation was to study the effect of substances shed by T. cruzi on ischemia/reperfusion-induced arrhythmias. We performed a triple ischemia-reperfusion (I/R) protocol whereby the isolated beating rat hearts were perfused with either Vero-control or Vero T. cruzi-infected conditioned medium during the different stages of ischemia and subsequently reperfused with Tyrode's solution. ECG and heart rate were recorded during the entire experiment. We observed that triple I/R-induced bradycardia was associated with the generation of auricular-ventricular blockade during ischemia and non-sustained nodal and ventricular tachycardia during reperfusion. Interestingly, perfusion with Vero-infected medium produced a delay in the reperfusion-induced recovery of heart rate, increased the frequency of tachycardic events and induced ventricular fibrillation. These results suggest that the presence of parasite-shed substances in conditioned media enhances the arrhythmogenic effects that occur during the I/R protocol. |
| first_indexed | 2024-04-13T19:23:01Z |
| format | Article |
| id | doaj.art-02e518168347472cb5c542129f48ef45 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-13T19:23:01Z |
| publishDate | 2013-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-02e518168347472cb5c542129f48ef452022-12-22T02:33:26ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2013-01-01461586410.1590/1414-431X20122409S0100-879X2013000100058Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned mediumH. Rodriguez-AnguloJ. Toro-MendozaJ. MarquesR. Bonfante-CabarcasA. MijaresChagas' myocardiopathy, caused by the intracellular protozoan Trypanosoma cruzi, is characterized by microvascular alterations, heart failure and arrhythmias. Ischemia and arrythmogenesis have been attributed to proteins shed by the parasite, although this has not been fully demonstrated. The aim of the present investigation was to study the effect of substances shed by T. cruzi on ischemia/reperfusion-induced arrhythmias. We performed a triple ischemia-reperfusion (I/R) protocol whereby the isolated beating rat hearts were perfused with either Vero-control or Vero T. cruzi-infected conditioned medium during the different stages of ischemia and subsequently reperfused with Tyrode's solution. ECG and heart rate were recorded during the entire experiment. We observed that triple I/R-induced bradycardia was associated with the generation of auricular-ventricular blockade during ischemia and non-sustained nodal and ventricular tachycardia during reperfusion. Interestingly, perfusion with Vero-infected medium produced a delay in the reperfusion-induced recovery of heart rate, increased the frequency of tachycardic events and induced ventricular fibrillation. These results suggest that the presence of parasite-shed substances in conditioned media enhances the arrhythmogenic effects that occur during the I/R protocol.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100058&lng=en&tlng=enChagas' diseaseArrhythmiasIschemia |
| spellingShingle | H. Rodriguez-Angulo J. Toro-Mendoza J. Marques R. Bonfante-Cabarcas A. Mijares Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium Brazilian Journal of Medical and Biological Research Chagas' disease Arrhythmias Ischemia |
| title | Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium |
| title_full | Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium |
| title_fullStr | Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium |
| title_full_unstemmed | Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium |
| title_short | Induction of chagasic-like arrhythmias in the isolated beating hearts of healthy rats perfused with Trypanosoma cruzi-conditioned medium |
| title_sort | induction of chagasic like arrhythmias in the isolated beating hearts of healthy rats perfused with trypanosoma cruzi conditioned medium |
| topic | Chagas' disease Arrhythmias Ischemia |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100058&lng=en&tlng=en |
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