Differences in senescence of late Endothelial Progenitor Cells in non-smokers and smokers
Introduction Endothelial Progenitor Cells (EPCs) are part of hematopoietic stem cells that differentiate into endothelial cells during their blood vessels’ maturation process. The role of EPCs is widely known to contribute to repair of the vascular wall when endothelial dysfunction occurs. However,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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European Publishing
2021-06-01
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| Series: | Tobacco Induced Diseases |
| Subjects: | |
| Online Access: | http://www.tobaccoinduceddiseases.org/Differences-in-senescence-of-late-Endothelial-Progenitor-Cells-nin-non-smokers-and,135320,0,2.html |
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| author | Kumboyono Kumboyono Indah Nur Chomsy Wiwit Nurwidyaningtyas Fibe Yulinda Cesa Cholid Tri Tjahjono Titin Andri Wihastuti |
| author_facet | Kumboyono Kumboyono Indah Nur Chomsy Wiwit Nurwidyaningtyas Fibe Yulinda Cesa Cholid Tri Tjahjono Titin Andri Wihastuti |
| author_sort | Kumboyono Kumboyono |
| collection | DOAJ |
| description | Introduction
Endothelial Progenitor Cells (EPCs) are part of hematopoietic stem
cells that differentiate into endothelial cells during their blood vessels’ maturation
process. The role of EPCs is widely known to contribute to repair of the vascular
wall when endothelial dysfunction occurs. However, various risk factors for
cardiovascular disease (CVD) influence EPC performance, leading to endothelial
dysfunction. One EPC dysfunction is decreased amount of EPC mobilization to the
injured tissue. EPC dysfunction reduces the angiogenetic function of EPCs. The
vital maturation process that the EPCs must pass is the late phase. The dysfunction
of late EPCs is known as senescence. This study aimed to identify and compare
senescence of late EPCs, through CD62E and CD41 markers, in non-smokers and
smokers as a risk factor for CVD.
Methods
EPC collection was from peripheral mononuclear cells (PBMCs) in
non-smokers (n=30) and smokers (n=31). The EPCs were then marked by
CD62E/CD41 and senescence β-galactosidase assay using FACS. Identification
of senescence cells was based on fluorescence with DAPI.
Results
Positive percentage of late EPCs in non-smokers was not significantly
different from that in smokers (p=0.014). The number of senescent late EPCs in
smokers was higher than in non-smokers (p<0.0001).
Conclusions
Endothelial progenitor cells that experienced senescence in the smokers
showed EPC dysfunction, which resulted in decreased cell angiogenic function.
Further research is needed to explain the mechanism of re-endothelialization
failure in EPC dysfunction due to smoking. |
| first_indexed | 2024-12-13T14:53:26Z |
| format | Article |
| id | doaj.art-02e91f8cdb904e17975155d4017ad705 |
| institution | Directory Open Access Journal |
| issn | 1617-9625 |
| language | English |
| last_indexed | 2024-12-13T14:53:26Z |
| publishDate | 2021-06-01 |
| publisher | European Publishing |
| record_format | Article |
| series | Tobacco Induced Diseases |
| spelling | doaj.art-02e91f8cdb904e17975155d4017ad7052022-12-21T23:41:17ZengEuropean PublishingTobacco Induced Diseases1617-96252021-06-0119June1810.18332/tid/135320135320Differences in senescence of late Endothelial Progenitor Cells in non-smokers and smokersKumboyono Kumboyono0Indah Nur Chomsy1Wiwit Nurwidyaningtyas2Fibe Yulinda Cesa3Cholid Tri Tjahjono4Titin Andri Wihastuti5School of Nursing, Faculty of Medicine, University of Brawijaya, Malang, IndonesiaFaculty of Medicine, University of Brawijaya, Malang, IndonesiaFaculty of Medicine, University of Brawijaya, Malang, IndonesiaFaculty of Medicine, University of Brawijaya, Malang, IndonesiaDepartment of Cardiology, Faculty of Medicine, University of Brawijaya, Malang, IndonesiaDepartment of Basic Nursing Science, Faculty of Medicine, University of Brawijaya, Malang, IndonesiaIntroduction Endothelial Progenitor Cells (EPCs) are part of hematopoietic stem cells that differentiate into endothelial cells during their blood vessels’ maturation process. The role of EPCs is widely known to contribute to repair of the vascular wall when endothelial dysfunction occurs. However, various risk factors for cardiovascular disease (CVD) influence EPC performance, leading to endothelial dysfunction. One EPC dysfunction is decreased amount of EPC mobilization to the injured tissue. EPC dysfunction reduces the angiogenetic function of EPCs. The vital maturation process that the EPCs must pass is the late phase. The dysfunction of late EPCs is known as senescence. This study aimed to identify and compare senescence of late EPCs, through CD62E and CD41 markers, in non-smokers and smokers as a risk factor for CVD. Methods EPC collection was from peripheral mononuclear cells (PBMCs) in non-smokers (n=30) and smokers (n=31). The EPCs were then marked by CD62E/CD41 and senescence β-galactosidase assay using FACS. Identification of senescence cells was based on fluorescence with DAPI. Results Positive percentage of late EPCs in non-smokers was not significantly different from that in smokers (p=0.014). The number of senescent late EPCs in smokers was higher than in non-smokers (p<0.0001). Conclusions Endothelial progenitor cells that experienced senescence in the smokers showed EPC dysfunction, which resulted in decreased cell angiogenic function. Further research is needed to explain the mechanism of re-endothelialization failure in EPC dysfunction due to smoking.http://www.tobaccoinduceddiseases.org/Differences-in-senescence-of-late-Endothelial-Progenitor-Cells-nin-non-smokers-and,135320,0,2.htmlprogenitor cellslate epcsepc dysfunctionsenescencesmoking |
| spellingShingle | Kumboyono Kumboyono Indah Nur Chomsy Wiwit Nurwidyaningtyas Fibe Yulinda Cesa Cholid Tri Tjahjono Titin Andri Wihastuti Differences in senescence of late Endothelial Progenitor Cells in non-smokers and smokers Tobacco Induced Diseases progenitor cells late epcs epc dysfunction senescence smoking |
| title | Differences in senescence of late Endothelial Progenitor Cells
in non-smokers and smokers |
| title_full | Differences in senescence of late Endothelial Progenitor Cells
in non-smokers and smokers |
| title_fullStr | Differences in senescence of late Endothelial Progenitor Cells
in non-smokers and smokers |
| title_full_unstemmed | Differences in senescence of late Endothelial Progenitor Cells
in non-smokers and smokers |
| title_short | Differences in senescence of late Endothelial Progenitor Cells
in non-smokers and smokers |
| title_sort | differences in senescence of late endothelial progenitor cells in non smokers and smokers |
| topic | progenitor cells late epcs epc dysfunction senescence smoking |
| url | http://www.tobaccoinduceddiseases.org/Differences-in-senescence-of-late-Endothelial-Progenitor-Cells-nin-non-smokers-and,135320,0,2.html |
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