Serratamolide is a hemolytic factor produced by Serratia marcescens.
Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A g...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3353980?pdf=render |
| _version_ | 1819077275874754560 |
|---|---|
| author | Robert M Q Shanks Nicholas A Stella Roni M Lahr Shaoru Wang Tara I Veverka Regis P Kowalski Xinyu Liu |
| author_facet | Robert M Q Shanks Nicholas A Stella Roni M Lahr Shaoru Wang Tara I Veverka Regis P Kowalski Xinyu Liu |
| author_sort | Robert M Q Shanks |
| collection | DOAJ |
| description | Serratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use. |
| first_indexed | 2024-12-21T18:54:37Z |
| format | Article |
| id | doaj.art-02e9ca0e76f6469bb2c4379bed9f5d85 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T18:54:37Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-02e9ca0e76f6469bb2c4379bed9f5d852022-12-21T18:53:38ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3639810.1371/journal.pone.0036398Serratamolide is a hemolytic factor produced by Serratia marcescens.Robert M Q ShanksNicholas A StellaRoni M LahrShaoru WangTara I VeverkaRegis P KowalskiXinyu LiuSerratia marcescens is a common contaminant of contact lens cases and lenses. Hemolytic factors of S. marcescens contribute to the virulence of this opportunistic bacterial pathogen. We took advantage of an observed hyper-hemolytic phenotype of crp mutants to investigate mechanisms of hemolysis. A genetic screen revealed that swrW is necessary for the hyper-hemolysis phenotype of crp mutants. The swrW gene is required for biosynthesis of the biosurfactant serratamolide, previously shown to be a broad-spectrum antibiotic and to contribute to swarming motility. Multicopy expression of swrW or mutation of the hexS transcription factor gene, a known inhibitor of swrW expression, led to an increase in hemolysis. Surfactant zones and expression from an swrW-transcriptional reporter were elevated in a crp mutant compared to the wild type. Purified serratamolide was hemolytic to sheep and murine red blood cells and cytotoxic to human airway and corneal limbal epithelial cells in vitro. The swrW gene was found in the majority of contact lens isolates tested. Genetic and biochemical analysis implicate the biosurfactant serratamolide as a hemolysin. This novel hemolysin may contribute to irritation and infections associated with contact lens use.http://europepmc.org/articles/PMC3353980?pdf=render |
| spellingShingle | Robert M Q Shanks Nicholas A Stella Roni M Lahr Shaoru Wang Tara I Veverka Regis P Kowalski Xinyu Liu Serratamolide is a hemolytic factor produced by Serratia marcescens. PLoS ONE |
| title | Serratamolide is a hemolytic factor produced by Serratia marcescens. |
| title_full | Serratamolide is a hemolytic factor produced by Serratia marcescens. |
| title_fullStr | Serratamolide is a hemolytic factor produced by Serratia marcescens. |
| title_full_unstemmed | Serratamolide is a hemolytic factor produced by Serratia marcescens. |
| title_short | Serratamolide is a hemolytic factor produced by Serratia marcescens. |
| title_sort | serratamolide is a hemolytic factor produced by serratia marcescens |
| url | http://europepmc.org/articles/PMC3353980?pdf=render |
| work_keys_str_mv | AT robertmqshanks serratamolideisahemolyticfactorproducedbyserratiamarcescens AT nicholasastella serratamolideisahemolyticfactorproducedbyserratiamarcescens AT ronimlahr serratamolideisahemolyticfactorproducedbyserratiamarcescens AT shaoruwang serratamolideisahemolyticfactorproducedbyserratiamarcescens AT taraiveverka serratamolideisahemolyticfactorproducedbyserratiamarcescens AT regispkowalski serratamolideisahemolyticfactorproducedbyserratiamarcescens AT xinyuliu serratamolideisahemolyticfactorproducedbyserratiamarcescens |