Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis
Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of micro...
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| Format: | Article |
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MDPI AG
2023-06-01
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| Series: | Birds |
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| Online Access: | https://www.mdpi.com/2673-6004/4/2/20 |
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| author | Marcelo Santos de Souza Suziane Alves Barcellos Victoria Tura Vera Lúcia Bobrowski Analía Del Valle Garnero Ricardo José Gunski Darren K. Griffin Rafael Kretschmer |
| author_facet | Marcelo Santos de Souza Suziane Alves Barcellos Victoria Tura Vera Lúcia Bobrowski Analía Del Valle Garnero Ricardo José Gunski Darren K. Griffin Rafael Kretschmer |
| author_sort | Marcelo Santos de Souza |
| collection | DOAJ |
| description | Passeriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of microchromosomes. Although the karyotype is typically 2n = 80, the diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed to understand the extent of conservation of the karyotype’s organizational structure within four species of this group using Bacterial Artificial Chromosomes via Fluorescence In Situ Hybridization (BAC-FISH) with microchromosome probes from Chicken (<i>Gallus gallus</i>) or Zebra Finch (<i>Taeniopygia guttata</i>) per microchromosomes (GGA10-28, except GGA16). By examining the chromosome complement of four passerine species—the Streaked Flycatcher (<i>Myiodynastes maculatus</i>), Shiny Cowbird (<i>Molothrus bonariensis</i>), Southern House Wren (<i>Troglodytes aedon</i>), and Double-collared Seedeater (<i>Sporophila caerulescens</i>)—we discovered a new chromosome number for Southern House Wren. Through FISH experiments, we were able to observe the same pattern of microchromosome organization as in the common ancestor of birds. As a result, we propose a new diploid number for Southern House Wren and confirm the conservation status of microchromosome organization, which may confer evolutionary advantages to this group. |
| first_indexed | 2024-03-11T02:41:24Z |
| format | Article |
| id | doaj.art-02ea7ad6062b4ca78d5fc01977ad8b52 |
| institution | Directory Open Access Journal |
| issn | 2673-6004 |
| language | English |
| last_indexed | 2024-03-11T02:41:24Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Birds |
| spelling | doaj.art-02ea7ad6062b4ca78d5fc01977ad8b522023-11-18T09:33:51ZengMDPI AGBirds2673-60042023-06-014223624410.3390/birds4020020Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH AnalysisMarcelo Santos de Souza0Suziane Alves Barcellos1Victoria Tura2Vera Lúcia Bobrowski3Analía Del Valle Garnero4Ricardo José Gunski5Darren K. Griffin6Rafael Kretschmer7Programa de Pós-Graduação em Ciências Biológicas (PPGCB), Universidade Federal do Pampa, São Gabriel 97300-000, RS, BrazilPrograma de Pós-Graduação em Ciências Biológicas (PPGCB), Universidade Federal do Pampa, São Gabriel 97300-000, RS, BrazilPrograma de Pós-Graduação em Ciências Biológicas (PPGCB), Universidade Federal do Pampa, São Gabriel 97300-000, RS, BrazilDepartamento de Ecologia, Zoologia e Genética, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas 96010-900, RS, BrazilPrograma de Pós-Graduação em Ciências Biológicas (PPGCB), Universidade Federal do Pampa, São Gabriel 97300-000, RS, BrazilPrograma de Pós-Graduação em Ciências Biológicas (PPGCB), Universidade Federal do Pampa, São Gabriel 97300-000, RS, BrazilSchool of Biosciences, University of Kent, Canterbury CT2 7NJ, UKDepartamento de Ecologia, Zoologia e Genética, Instituto de Biologia, Universidade Federal de Pelotas, Pelotas 96010-900, RS, BrazilPasseriformes birds are widely recognized for their remarkable diversity, with over 5700 species described so far. Like most bird species, they possess a karyotype characteristic of modern birds, which includes a bimodal karyotype consisting of a few pairs of macrochromosomes and many pairs of microchromosomes. Although the karyotype is typically 2n = 80, the diploid number can atypically vary greatly, ranging from 56 to approximately 100 chromosomes. In this study, we aimed to understand the extent of conservation of the karyotype’s organizational structure within four species of this group using Bacterial Artificial Chromosomes via Fluorescence In Situ Hybridization (BAC-FISH) with microchromosome probes from Chicken (<i>Gallus gallus</i>) or Zebra Finch (<i>Taeniopygia guttata</i>) per microchromosomes (GGA10-28, except GGA16). By examining the chromosome complement of four passerine species—the Streaked Flycatcher (<i>Myiodynastes maculatus</i>), Shiny Cowbird (<i>Molothrus bonariensis</i>), Southern House Wren (<i>Troglodytes aedon</i>), and Double-collared Seedeater (<i>Sporophila caerulescens</i>)—we discovered a new chromosome number for Southern House Wren. Through FISH experiments, we were able to observe the same pattern of microchromosome organization as in the common ancestor of birds. As a result, we propose a new diploid number for Southern House Wren and confirm the conservation status of microchromosome organization, which may confer evolutionary advantages to this group.https://www.mdpi.com/2673-6004/4/2/20Avesdiploid numberkaryotype organizationmolecular cytogenetic |
| spellingShingle | Marcelo Santos de Souza Suziane Alves Barcellos Victoria Tura Vera Lúcia Bobrowski Analía Del Valle Garnero Ricardo José Gunski Darren K. Griffin Rafael Kretschmer Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis Birds Aves diploid number karyotype organization molecular cytogenetic |
| title | Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis |
| title_full | Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis |
| title_fullStr | Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis |
| title_full_unstemmed | Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis |
| title_short | Highly Conserved Microchromosomal Organization in Passeriformes Birds Revealed via BAC-FISH Analysis |
| title_sort | highly conserved microchromosomal organization in passeriformes birds revealed via bac fish analysis |
| topic | Aves diploid number karyotype organization molecular cytogenetic |
| url | https://www.mdpi.com/2673-6004/4/2/20 |
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