Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development
Abstract Background The challenge of pigeonpea breeding lies in its photosensitivity and seasonal specificity. This poses a problem to the breeder, as it restricts to single generation advancement in a year. Currently, the cross to cultivar gap is twelve to thirteen years resulting in a limited numb...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-02-01
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| Series: | Plant Methods |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13007-024-01155-w |
| _version_ | 1797274418106335232 |
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| author | Prakash I. Gangashetty Shruthi H. Belliappa Naresh Bomma Vinutha Kanuganahalli Sobhan Babu Sajja Sunita Choudhary Ramanagouda Gaviyappanavar Deekshitha Bomireddy V. Anil Kumar Jwala Pranati Mamta Sharma Manish K. Pandey |
| author_facet | Prakash I. Gangashetty Shruthi H. Belliappa Naresh Bomma Vinutha Kanuganahalli Sobhan Babu Sajja Sunita Choudhary Ramanagouda Gaviyappanavar Deekshitha Bomireddy V. Anil Kumar Jwala Pranati Mamta Sharma Manish K. Pandey |
| author_sort | Prakash I. Gangashetty |
| collection | DOAJ |
| description | Abstract Background The challenge of pigeonpea breeding lies in its photosensitivity and seasonal specificity. This poses a problem to the breeder, as it restricts to single generation advancement in a year. Currently, the cross to cultivar gap is twelve to thirteen years resulting in a limited number of varietal releases over the past six decades. Shortening the breeding cycle was need of the hour, unlikely achieved by conventional breeding. To overcome these hindrances speed breeding was a necessary leap. An experiment was planned to optimize the speed breeding coupled with single seed descent and seed or pod chip-based genotyping to shorten the breeding cycle in pigeonpea at ICRISAT, Hyderabad. Monitored photoperiod, light wavelength, temperature and crop management regime were the indicators attributing to the success of speed breeding. Result A photoperiod of 13 h: 8 h: 13 h at vegetative: flowering and pod filling stages is ideal for shortening the breeding cycle. Broad spectrum light (5700 K LED) hastened early vegetative growth and pod formation. Whereas far-red (735 nm) light favoured early flowering. A significant difference between the photoperiods, genotypes as well as photoperiod x genotype interaction for both days to flowering and plant height was noted. Conclusion The optimized protocol serves as a road map for rapid generation advancement in pigeonpea. Deploying this protocol, it is possible to advance 2–4 generations per year. The breeding cycle can be reduced to 2–4 years which otherwise takes 7 years under conventional breeding. Single Seed Descent and seed or pod chip-based genotyping for early generation marker assisted selection, strengthened the precision of this technique aiding in high throughput line development. |
| first_indexed | 2024-03-07T14:59:03Z |
| format | Article |
| id | doaj.art-02ebfc5009754087a3f4c1240f142566 |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-03-07T14:59:03Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-02ebfc5009754087a3f4c1240f1425662024-03-05T19:15:24ZengBMCPlant Methods1746-48112024-02-0120111210.1186/s13007-024-01155-wOptimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line developmentPrakash I. Gangashetty0Shruthi H. Belliappa1Naresh Bomma2Vinutha Kanuganahalli3Sobhan Babu Sajja4Sunita Choudhary5Ramanagouda Gaviyappanavar6Deekshitha Bomireddy7V. Anil Kumar8Jwala Pranati9Mamta Sharma10Manish K. Pandey11International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)International Crops Research Institute for the Semi-Arid tropics (ICRISAT)Abstract Background The challenge of pigeonpea breeding lies in its photosensitivity and seasonal specificity. This poses a problem to the breeder, as it restricts to single generation advancement in a year. Currently, the cross to cultivar gap is twelve to thirteen years resulting in a limited number of varietal releases over the past six decades. Shortening the breeding cycle was need of the hour, unlikely achieved by conventional breeding. To overcome these hindrances speed breeding was a necessary leap. An experiment was planned to optimize the speed breeding coupled with single seed descent and seed or pod chip-based genotyping to shorten the breeding cycle in pigeonpea at ICRISAT, Hyderabad. Monitored photoperiod, light wavelength, temperature and crop management regime were the indicators attributing to the success of speed breeding. Result A photoperiod of 13 h: 8 h: 13 h at vegetative: flowering and pod filling stages is ideal for shortening the breeding cycle. Broad spectrum light (5700 K LED) hastened early vegetative growth and pod formation. Whereas far-red (735 nm) light favoured early flowering. A significant difference between the photoperiods, genotypes as well as photoperiod x genotype interaction for both days to flowering and plant height was noted. Conclusion The optimized protocol serves as a road map for rapid generation advancement in pigeonpea. Deploying this protocol, it is possible to advance 2–4 generations per year. The breeding cycle can be reduced to 2–4 years which otherwise takes 7 years under conventional breeding. Single Seed Descent and seed or pod chip-based genotyping for early generation marker assisted selection, strengthened the precision of this technique aiding in high throughput line development.https://doi.org/10.1186/s13007-024-01155-wSpeed breedingPhotoperiodSeed or pod chip genotypingSingle seed descent |
| spellingShingle | Prakash I. Gangashetty Shruthi H. Belliappa Naresh Bomma Vinutha Kanuganahalli Sobhan Babu Sajja Sunita Choudhary Ramanagouda Gaviyappanavar Deekshitha Bomireddy V. Anil Kumar Jwala Pranati Mamta Sharma Manish K. Pandey Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development Plant Methods Speed breeding Photoperiod Seed or pod chip genotyping Single seed descent |
| title | Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development |
| title_full | Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development |
| title_fullStr | Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development |
| title_full_unstemmed | Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development |
| title_short | Optimizing speed breeding and seed/pod chip based genotyping techniques in pigeonpea: A way forward for high throughput line development |
| title_sort | optimizing speed breeding and seed pod chip based genotyping techniques in pigeonpea a way forward for high throughput line development |
| topic | Speed breeding Photoperiod Seed or pod chip genotyping Single seed descent |
| url | https://doi.org/10.1186/s13007-024-01155-w |
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