Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and Phytopathogens
In this study, seven different silver nanoparticles (AgNPs) were obtained using the fungi species from the phylum Ascomycota, <i>Aspergillus tubingensis</i>, <i>Aspergillus</i> spp., <i>Cladosporium pini-ponderosae</i>, <i>Fusarium proliferatum</i>, &l...
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MDPI AG
2023-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/1/91 |
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| author | Luiz Gustavo Ribeiro Gabriella Sales Calaço Roque Rafael Conrado Ana Olívia De Souza |
| author_facet | Luiz Gustavo Ribeiro Gabriella Sales Calaço Roque Rafael Conrado Ana Olívia De Souza |
| author_sort | Luiz Gustavo Ribeiro |
| collection | DOAJ |
| description | In this study, seven different silver nanoparticles (AgNPs) were obtained using the fungi species from the phylum Ascomycota, <i>Aspergillus tubingensis</i>, <i>Aspergillus</i> spp., <i>Cladosporium pini-ponderosae</i>, <i>Fusarium proliferatum</i>, <i>Epicoccum nigrum</i>, <i>Exserohilum rostratum</i>, and <i>Bionectria ochroleuca</i>, isolated from the Brazilian biodiversity, particularly from the mangrove and Caatinga biomes. The nanoparticles were coded as AgNP-AT, AgNP-Asp, AgNP-CPP, AgNP-FP, AgNP-EN, AgNP-ER, and AgNP-BO and characterized using spectrophotometry (UV-Vis), dynamic light scattering (DLS), zeta potential, transmission electron microcopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy. All the AgNPs presented homogeneous size in the range from 43.4 to 120.6 nm (DLS) and from 21.8 to 35.8 nm (TEM), pH from 4.5 to 7.5, negative charge, and presence of protein coating on their surface. The antifungal activity of the AgNPs was evaluated on clinical strains of <i>Candida albicans</i>, and on the non-albicans species, <i>Candida krusei</i>, <i>Candida glabrata</i>, <i>Candida parapsilosis</i>, <i>Candida tropicalis</i>, and <i>Candida guilliermondii</i>, common in hospital infections, and against the phytopathogens <i>Fusarium oxysporum, Fusarium phaseoli</i>, <i>Fusarium sacchari</i>, <i>Fusarium subglutinans</i>, <i>Fusarium verticillioides</i>, and <i>Curvularia lunata</i>, which are species responsible for serious damage to agriculture production. The AgNPs were effective against the yeasts with MICs ranging from 1.25 to 40 µM and on the phytopathogens with MICs from 4 to 250 µM, indicating the promising possibility of application of these AgNPs as antifungal agents. The results indicated that the physicochemical parameters of the AgNPs, including the functional groups present on their surface, interfered with their antifungal activity. Overall, the results indicate that there is no specificity of the AgNPs for the yeasts or for the phytopathogens, which can be an advantage, increasing the possibility of application in different areas. |
| first_indexed | 2024-03-09T13:47:44Z |
| format | Article |
| id | doaj.art-02ee186732fe4cc3bf50f72199c5d89d |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T13:47:44Z |
| publishDate | 2023-01-01 |
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| spelling | doaj.art-02ee186732fe4cc3bf50f72199c5d89d2023-11-30T20:55:34ZengMDPI AGAntibiotics2079-63822023-01-011219110.3390/antibiotics12010091Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and PhytopathogensLuiz Gustavo Ribeiro0Gabriella Sales Calaço Roque1Rafael Conrado2Ana Olívia De Souza3Development and Innovation Laboratory, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, BrazilDevelopment and Innovation Laboratory, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, BrazilDevelopment and Innovation Laboratory, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, BrazilDevelopment and Innovation Laboratory, Instituto Butantan, Avenida Vital Brasil, 1500, São Paulo 05503-900, SP, BrazilIn this study, seven different silver nanoparticles (AgNPs) were obtained using the fungi species from the phylum Ascomycota, <i>Aspergillus tubingensis</i>, <i>Aspergillus</i> spp., <i>Cladosporium pini-ponderosae</i>, <i>Fusarium proliferatum</i>, <i>Epicoccum nigrum</i>, <i>Exserohilum rostratum</i>, and <i>Bionectria ochroleuca</i>, isolated from the Brazilian biodiversity, particularly from the mangrove and Caatinga biomes. The nanoparticles were coded as AgNP-AT, AgNP-Asp, AgNP-CPP, AgNP-FP, AgNP-EN, AgNP-ER, and AgNP-BO and characterized using spectrophotometry (UV-Vis), dynamic light scattering (DLS), zeta potential, transmission electron microcopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy. All the AgNPs presented homogeneous size in the range from 43.4 to 120.6 nm (DLS) and from 21.8 to 35.8 nm (TEM), pH from 4.5 to 7.5, negative charge, and presence of protein coating on their surface. The antifungal activity of the AgNPs was evaluated on clinical strains of <i>Candida albicans</i>, and on the non-albicans species, <i>Candida krusei</i>, <i>Candida glabrata</i>, <i>Candida parapsilosis</i>, <i>Candida tropicalis</i>, and <i>Candida guilliermondii</i>, common in hospital infections, and against the phytopathogens <i>Fusarium oxysporum, Fusarium phaseoli</i>, <i>Fusarium sacchari</i>, <i>Fusarium subglutinans</i>, <i>Fusarium verticillioides</i>, and <i>Curvularia lunata</i>, which are species responsible for serious damage to agriculture production. The AgNPs were effective against the yeasts with MICs ranging from 1.25 to 40 µM and on the phytopathogens with MICs from 4 to 250 µM, indicating the promising possibility of application of these AgNPs as antifungal agents. The results indicated that the physicochemical parameters of the AgNPs, including the functional groups present on their surface, interfered with their antifungal activity. Overall, the results indicate that there is no specificity of the AgNPs for the yeasts or for the phytopathogens, which can be an advantage, increasing the possibility of application in different areas.https://www.mdpi.com/2079-6382/12/1/91biogenic silver nanoparticlesphytopathogens<i>Candida</i> sp.antifungal activity |
| spellingShingle | Luiz Gustavo Ribeiro Gabriella Sales Calaço Roque Rafael Conrado Ana Olívia De Souza Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and Phytopathogens Antibiotics biogenic silver nanoparticles phytopathogens <i>Candida</i> sp. antifungal activity |
| title | Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and Phytopathogens |
| title_full | Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and Phytopathogens |
| title_fullStr | Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and Phytopathogens |
| title_full_unstemmed | Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and Phytopathogens |
| title_short | Antifungal Activity of Mycogenic Silver Nanoparticles on Clinical Yeasts and Phytopathogens |
| title_sort | antifungal activity of mycogenic silver nanoparticles on clinical yeasts and phytopathogens |
| topic | biogenic silver nanoparticles phytopathogens <i>Candida</i> sp. antifungal activity |
| url | https://www.mdpi.com/2079-6382/12/1/91 |
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