Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
A large number of Endotrypanum stocks (representing an heterogeneous population of strains) have been screened against a panel of monoclonal antibodies (MAbs) derived for selected species of Endotrypanum or Leishmania, to see whether this approach could be used to group/differentiate further among t...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1997-01-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100013 |
| _version_ | 1827842495529091072 |
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| author | Ramos Franco Antonia Maria Machado Gerzia MC Naiff Roberto D Moreira Celia FS McMahon-Pratt Diane Grimaldi Jr Gabriel |
| author_facet | Ramos Franco Antonia Maria Machado Gerzia MC Naiff Roberto D Moreira Celia FS McMahon-Pratt Diane Grimaldi Jr Gabriel |
| author_sort | Ramos Franco Antonia Maria |
| collection | DOAJ |
| description | A large number of Endotrypanum stocks (representing an heterogeneous population of strains) have been screened against a panel of monoclonal antibodies (MAbs) derived for selected species of Endotrypanum or Leishmania, to see whether this approach could be used to group/differentiate further among these parasites. Using different immunological assay systems, MAbs considered specific for the genus Endotrypanum (E-24, CXXX-3G5-F12) or strain M6159 of E. schaudinni (E-2, CXIV-3C7-F5) reacted variably according to the test used but in the ELISA or immunofluorescence assay both reacted with all the strains tested. Analyses using these MAbs showed antigenic diversity occurring among the Endotrypanum strains, but no qualitative or quantitative reactivity pattern could be consistently related to parasite origin (i.e., host species involved) or geographic area of isolation. Western blot analyses of the parasites showed that these MAbs recognized multiple components. Differences existed either in the epitope density or molecular forms associated with the antigenic determinants and therefore allowed the assignment of the strains to specific antigenic groups. Using immunofluorescence or ELISA assay, clone E-24 produced reaction with L. equatorensis (which is a parasite of sloth and rodent), but not with other trypanosomatids examined. Interestingly, the latter parasite and the Endotrypanum strains cross-reacted with a number of MAbs that were produced against members of the L. major-L. tropica complex |
| first_indexed | 2024-03-12T08:10:22Z |
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| id | doaj.art-02f094abb30c4198bfd27484b9847501 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:10:22Z |
| publishDate | 1997-01-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-02f094abb30c4198bfd27484b98475012023-09-02T19:07:06ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601997-01-019216368Characterization of Endotrypanum Parasites Using Specific Monoclonal AntibodiesRamos Franco Antonia MariaMachado Gerzia MCNaiff Roberto DMoreira Celia FSMcMahon-Pratt DianeGrimaldi Jr GabrielA large number of Endotrypanum stocks (representing an heterogeneous population of strains) have been screened against a panel of monoclonal antibodies (MAbs) derived for selected species of Endotrypanum or Leishmania, to see whether this approach could be used to group/differentiate further among these parasites. Using different immunological assay systems, MAbs considered specific for the genus Endotrypanum (E-24, CXXX-3G5-F12) or strain M6159 of E. schaudinni (E-2, CXIV-3C7-F5) reacted variably according to the test used but in the ELISA or immunofluorescence assay both reacted with all the strains tested. Analyses using these MAbs showed antigenic diversity occurring among the Endotrypanum strains, but no qualitative or quantitative reactivity pattern could be consistently related to parasite origin (i.e., host species involved) or geographic area of isolation. Western blot analyses of the parasites showed that these MAbs recognized multiple components. Differences existed either in the epitope density or molecular forms associated with the antigenic determinants and therefore allowed the assignment of the strains to specific antigenic groups. Using immunofluorescence or ELISA assay, clone E-24 produced reaction with L. equatorensis (which is a parasite of sloth and rodent), but not with other trypanosomatids examined. Interestingly, the latter parasite and the Endotrypanum strains cross-reacted with a number of MAbs that were produced against members of the L. major-L. tropica complexhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100013EndotrypanumKinetoplastidaTrypanosomatidaemammalian reservoirsmolecular characterizationmonoclonal antibodies |
| spellingShingle | Ramos Franco Antonia Maria Machado Gerzia MC Naiff Roberto D Moreira Celia FS McMahon-Pratt Diane Grimaldi Jr Gabriel Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies Memorias do Instituto Oswaldo Cruz Endotrypanum Kinetoplastida Trypanosomatidae mammalian reservoirs molecular characterization monoclonal antibodies |
| title | Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies |
| title_full | Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies |
| title_fullStr | Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies |
| title_full_unstemmed | Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies |
| title_short | Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies |
| title_sort | characterization of endotrypanum parasites using specific monoclonal antibodies |
| topic | Endotrypanum Kinetoplastida Trypanosomatidae mammalian reservoirs molecular characterization monoclonal antibodies |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000100013 |
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