Isoflavones hydrolisis and extraction
Isoflavones are found in leguminous species and are used as phytoestrogens widely used by industry for its beneficial effects as estrogens mimicked, antioxidant action and anti-cancer activity. The identification and quantification of isoflavones in plants is a need due to the high demand of industr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Zootecnia
2012-12-01
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| Series: | Boletim de Indústria Animal |
| Online Access: | http://revistas.bvs-vet.org.br/bia/article/view/7875 |
| _version_ | 1818994470184550400 |
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| author | Jozilene Fernandes Farias dos Santos Keila Maria Roncato Duarte Fernanda Ferreira Pessoa Marcia Atauri Cardelli de Lucena |
| author_facet | Jozilene Fernandes Farias dos Santos Keila Maria Roncato Duarte Fernanda Ferreira Pessoa Marcia Atauri Cardelli de Lucena |
| author_sort | Jozilene Fernandes Farias dos Santos |
| collection | DOAJ |
| description | Isoflavones are found in leguminous species and are used as phytoestrogens widely used by industry for its beneficial effects as estrogens mimicked, antioxidant action and anti-cancer activity. The identification and quantification of isoflavones in plants is a need due to the high demand of industry. Several methods are used for its extraction, using organic solvents (methanol, ethanol and acetonitrile). Samples from five legumes species from Instituto de Zootecnia (IZ), Forage Gene Bank were tested. All seeds received a hydrothermic treatment immersed in pure water at 50°C for 12 hours. Seeds were then oven-dryed. In this work we tested the extraction using only the hydrothermic treatment and hyfrothermic treatment allied to methanol extaction protocol. Seeds were grinded and half of the samples were ressuspended in PBS (phosphate Buffer) and the other half were submited to 4 mL of methanol and 1% of acetic acid, soaked for 5 hours, shaked every 15 minutes, at room temperature. The five legume species that we quantify isoflavones by enzyme immunoassay (EIA) were: Calopogonium mucunoides, Bauhinia sp., Cajanus cajan, Galactia martii, Leucaena leucocephala. The extraction procedure is a recomendation of AOAC (Association of Official Analytical Chemists) for isoflavone quantification. Ours results show an increase of extraction using methanol 80% plus acetic acid 1% and was obtained using solvent extraction in comparison to hydrothermic procedure alone (figure 1). |
| first_indexed | 2024-12-20T20:58:27Z |
| format | Article |
| id | doaj.art-02f19cbe84194b879a61b8943f4235f6 |
| institution | Directory Open Access Journal |
| issn | 1981-4100 |
| language | English |
| last_indexed | 2024-12-20T20:58:27Z |
| publishDate | 2012-12-01 |
| publisher | Instituto de Zootecnia |
| record_format | Article |
| series | Boletim de Indústria Animal |
| spelling | doaj.art-02f19cbe84194b879a61b8943f4235f62022-12-21T19:26:45ZengInstituto de ZootecniaBoletim de Indústria Animal1981-41002012-12-0169supl.47477075Isoflavones hydrolisis and extractionJozilene Fernandes Farias dos Santos0Keila Maria Roncato Duarte1Fernanda Ferreira Pessoa2Marcia Atauri Cardelli de Lucena3Secretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Nutrição Animal e Pastagem, Nova Odessa, SPSecretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Nutrição Animal e Pastagem, Nova Odessa, SPSecretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Nutrição Animal e Pastagem, Nova Odessa, SPSecretaria de Agricultura e Abastecimento do Estado de São Paulo, Agência Paulista de Tecnologia dos Agronegócios, Instituto de Zootecnia, Centro de Pesquisa e Desenvolvimento em Nutrição Animal e Pastagem, Nova Odessa, SPIsoflavones are found in leguminous species and are used as phytoestrogens widely used by industry for its beneficial effects as estrogens mimicked, antioxidant action and anti-cancer activity. The identification and quantification of isoflavones in plants is a need due to the high demand of industry. Several methods are used for its extraction, using organic solvents (methanol, ethanol and acetonitrile). Samples from five legumes species from Instituto de Zootecnia (IZ), Forage Gene Bank were tested. All seeds received a hydrothermic treatment immersed in pure water at 50°C for 12 hours. Seeds were then oven-dryed. In this work we tested the extraction using only the hydrothermic treatment and hyfrothermic treatment allied to methanol extaction protocol. Seeds were grinded and half of the samples were ressuspended in PBS (phosphate Buffer) and the other half were submited to 4 mL of methanol and 1% of acetic acid, soaked for 5 hours, shaked every 15 minutes, at room temperature. The five legume species that we quantify isoflavones by enzyme immunoassay (EIA) were: Calopogonium mucunoides, Bauhinia sp., Cajanus cajan, Galactia martii, Leucaena leucocephala. The extraction procedure is a recomendation of AOAC (Association of Official Analytical Chemists) for isoflavone quantification. Ours results show an increase of extraction using methanol 80% plus acetic acid 1% and was obtained using solvent extraction in comparison to hydrothermic procedure alone (figure 1).http://revistas.bvs-vet.org.br/bia/article/view/7875 |
| spellingShingle | Jozilene Fernandes Farias dos Santos Keila Maria Roncato Duarte Fernanda Ferreira Pessoa Marcia Atauri Cardelli de Lucena Isoflavones hydrolisis and extraction Boletim de Indústria Animal |
| title | Isoflavones hydrolisis and extraction |
| title_full | Isoflavones hydrolisis and extraction |
| title_fullStr | Isoflavones hydrolisis and extraction |
| title_full_unstemmed | Isoflavones hydrolisis and extraction |
| title_short | Isoflavones hydrolisis and extraction |
| title_sort | isoflavones hydrolisis and extraction |
| url | http://revistas.bvs-vet.org.br/bia/article/view/7875 |
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