Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor
The biosurfactant production is characterized by high costs with substrates, which does not make them sufficiently competitive against synthetic surfactants. The insertion of alternative sources of low cost, especially agro-industrial residue, is an excellent alternative to make this competitiveness...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-12-01
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| Series: | Biotechnology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X19301687 |
| _version_ | 1817984465885986816 |
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| author | Aline Alves Oliveira Santos Prado Brenda Lohanny Passos Santos Isabela Maria Monteiro Vieira Larissa Castor Ramos Roberto Rodrigues de Souza Daniel Pereira Silva Denise Santos Ruzene |
| author_facet | Aline Alves Oliveira Santos Prado Brenda Lohanny Passos Santos Isabela Maria Monteiro Vieira Larissa Castor Ramos Roberto Rodrigues de Souza Daniel Pereira Silva Denise Santos Ruzene |
| author_sort | Aline Alves Oliveira Santos Prado |
| collection | DOAJ |
| description | The biosurfactant production is characterized by high costs with substrates, which does not make them sufficiently competitive against synthetic surfactants. The insertion of alternative sources of low cost, especially agro-industrial residue, is an excellent alternative to make this competitiveness viable. An alkaline pretreatment was used to extract the hemicellulose from corncob in order to enhance its C5 fraction, common to vegetable biomasses. The hemicellulosic corncob liquor was used with glucose and mineral salt solution as carbon and nutrients sources in a fermentation process for the growth of Bacillus subtilis. It was performed a 23 full factorial design to determine the best conditions for the surfactin production in relation to the following response variables: surface tension reduction rate (STRR) and emulsification index (EI24), from which were obtained two optimized bioproducts under specific conditions. The optimized biosurfactants found to be effected presenting a critical micelle concentration of 100 mg.L−1 and a maximum bioremediation potential of 85.18%, as well as maximum values of 57.38% and 65.30% for STRR and EI24 variables, respectively. Overall results pointed for a successful commercial application for the surfactin produced. Keywords: Biosurfactant, Design of experiments, Lignocellulosic residue, Hemicellulosic corncob liquor, Bioremediation |
| first_indexed | 2024-04-13T23:44:59Z |
| format | Article |
| id | doaj.art-02f2168651954da2b4773bf064ddbb12 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-13T23:44:59Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-02f2168651954da2b4773bf064ddbb122022-12-22T02:24:21ZengElsevierBiotechnology Reports2215-017X2019-12-0124Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquorAline Alves Oliveira Santos Prado0Brenda Lohanny Passos Santos1Isabela Maria Monteiro Vieira2Larissa Castor Ramos3Roberto Rodrigues de Souza4Daniel Pereira Silva5Denise Santos Ruzene6Northeastern Biotechnology Network, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil; Coordination of Food Technical Course, Federal Institute of Sergipe, 49055-260, Aracaju, SE, BrazilCenter for Exact Sciences and Technology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, BrazilNortheastern Biotechnology Network, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil; Center for Exact Sciences and Technology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, BrazilNortheastern Biotechnology Network, Federal University of Sergipe, 49100-000, São Cristóvão, SE, BrazilNortheastern Biotechnology Network, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil; Center for Exact Sciences and Technology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, BrazilNortheastern Biotechnology Network, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil; Center for Exact Sciences and Technology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil; Corresponding author at: Federal University of Sergipe, Center for Exact Sciences and Technology, Rodovia Marechal Rondon, s/n, Jardim Rosa Elze, São Cristóvão, Sergipe 49100-000, Brazil.Northeastern Biotechnology Network, Federal University of Sergipe, 49100-000, São Cristóvão, SE, Brazil; Center for Exact Sciences and Technology, Federal University of Sergipe, 49100-000, São Cristóvão, SE, BrazilThe biosurfactant production is characterized by high costs with substrates, which does not make them sufficiently competitive against synthetic surfactants. The insertion of alternative sources of low cost, especially agro-industrial residue, is an excellent alternative to make this competitiveness viable. An alkaline pretreatment was used to extract the hemicellulose from corncob in order to enhance its C5 fraction, common to vegetable biomasses. The hemicellulosic corncob liquor was used with glucose and mineral salt solution as carbon and nutrients sources in a fermentation process for the growth of Bacillus subtilis. It was performed a 23 full factorial design to determine the best conditions for the surfactin production in relation to the following response variables: surface tension reduction rate (STRR) and emulsification index (EI24), from which were obtained two optimized bioproducts under specific conditions. The optimized biosurfactants found to be effected presenting a critical micelle concentration of 100 mg.L−1 and a maximum bioremediation potential of 85.18%, as well as maximum values of 57.38% and 65.30% for STRR and EI24 variables, respectively. Overall results pointed for a successful commercial application for the surfactin produced. Keywords: Biosurfactant, Design of experiments, Lignocellulosic residue, Hemicellulosic corncob liquor, Bioremediationhttp://www.sciencedirect.com/science/article/pii/S2215017X19301687 |
| spellingShingle | Aline Alves Oliveira Santos Prado Brenda Lohanny Passos Santos Isabela Maria Monteiro Vieira Larissa Castor Ramos Roberto Rodrigues de Souza Daniel Pereira Silva Denise Santos Ruzene Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor Biotechnology Reports |
| title | Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor |
| title_full | Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor |
| title_fullStr | Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor |
| title_full_unstemmed | Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor |
| title_short | Evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor |
| title_sort | evaluation of a new strategy in the elaboration of culture media to produce surfactin from hemicellulosic corncob liquor |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X19301687 |
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