A simple method to align cells on 3D hydrogels using 3D printed molds
Vascular smooth muscle cells align circumferentially around the vessel lumen, which allows these cells to control vascular tone by contracting and relaxing. It is essential that this circumferential alignment is recapitulated in tissue engineered blood vessels. While many methods have been reported...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | Biomedical Engineering Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667099221000013 |
| _version_ | 1818691502518304768 |
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| author | Jesse Vo Yusuf Mastoor Pattie S. Mathieu Alisa Morss Clyne |
| author_facet | Jesse Vo Yusuf Mastoor Pattie S. Mathieu Alisa Morss Clyne |
| author_sort | Jesse Vo |
| collection | DOAJ |
| description | Vascular smooth muscle cells align circumferentially around the vessel lumen, which allows these cells to control vascular tone by contracting and relaxing. It is essential that this circumferential alignment is recapitulated in tissue engineered blood vessels. While many methods have been reported to align cells on 2D polymeric substrates, few techniques enable cell alignment on a 3D physiologically relevant hydrogel substrate. We hypothesized that the ridges inherent to the sides of fused deposition modeling 3D printed molds could be used to topographically pattern both stiff and soft substrates and thereby align cells on flat and curved surfaces. Flat and curved molds with 150, 250, and 350 µm ridges were 3D printed and used to topographically pattern polydimethylsiloxane and gelatin-methacryloyl. The ridges transferred to both substrates with less than 10% change in ridge size. Vascular smooth muscle cells were then seeded on each substrate, and nuclear and actin alignment were quantified. Cells were highly aligned with the molded ridges to a similar extent on both the stiffer polydimethylsiloxane and the softer gelatin-methacryloyl substrates. These data confirm that fused deposition modeling 3D printed molds are a rapid, cost-effective way to topographically pattern stiff and soft substrates in varied 3D shapes. This method will enable investigators to align cells on 3D polymeric and hydrogel structures for tissue engineering and other applications. |
| first_indexed | 2024-12-17T12:42:55Z |
| format | Article |
| id | doaj.art-02f26e54e41148e3b319bcc7b96faad5 |
| institution | Directory Open Access Journal |
| issn | 2667-0992 |
| language | English |
| last_indexed | 2024-12-17T12:42:55Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Engineering Advances |
| spelling | doaj.art-02f26e54e41148e3b319bcc7b96faad52022-12-21T21:47:52ZengElsevierBiomedical Engineering Advances2667-09922021-06-011100001A simple method to align cells on 3D hydrogels using 3D printed moldsJesse Vo0Yusuf Mastoor1Pattie S. Mathieu2Alisa Morss Clyne3Fischell Department of Bioengineering University of Maryland 8278 Paint Branch Drive College Park, MD 20742, USAFischell Department of Bioengineering University of Maryland 8278 Paint Branch Drive College Park, MD 20742, USAFischell Department of Bioengineering University of Maryland 8278 Paint Branch Drive College Park, MD 20742, USACorresponding author.; Fischell Department of Bioengineering University of Maryland 8278 Paint Branch Drive College Park, MD 20742, USAVascular smooth muscle cells align circumferentially around the vessel lumen, which allows these cells to control vascular tone by contracting and relaxing. It is essential that this circumferential alignment is recapitulated in tissue engineered blood vessels. While many methods have been reported to align cells on 2D polymeric substrates, few techniques enable cell alignment on a 3D physiologically relevant hydrogel substrate. We hypothesized that the ridges inherent to the sides of fused deposition modeling 3D printed molds could be used to topographically pattern both stiff and soft substrates and thereby align cells on flat and curved surfaces. Flat and curved molds with 150, 250, and 350 µm ridges were 3D printed and used to topographically pattern polydimethylsiloxane and gelatin-methacryloyl. The ridges transferred to both substrates with less than 10% change in ridge size. Vascular smooth muscle cells were then seeded on each substrate, and nuclear and actin alignment were quantified. Cells were highly aligned with the molded ridges to a similar extent on both the stiffer polydimethylsiloxane and the softer gelatin-methacryloyl substrates. These data confirm that fused deposition modeling 3D printed molds are a rapid, cost-effective way to topographically pattern stiff and soft substrates in varied 3D shapes. This method will enable investigators to align cells on 3D polymeric and hydrogel structures for tissue engineering and other applications.http://www.sciencedirect.com/science/article/pii/S2667099221000013Vascular smooth muscle cellsCell alignmentHydrogel patterning3D printing |
| spellingShingle | Jesse Vo Yusuf Mastoor Pattie S. Mathieu Alisa Morss Clyne A simple method to align cells on 3D hydrogels using 3D printed molds Biomedical Engineering Advances Vascular smooth muscle cells Cell alignment Hydrogel patterning 3D printing |
| title | A simple method to align cells on 3D hydrogels using 3D printed molds |
| title_full | A simple method to align cells on 3D hydrogels using 3D printed molds |
| title_fullStr | A simple method to align cells on 3D hydrogels using 3D printed molds |
| title_full_unstemmed | A simple method to align cells on 3D hydrogels using 3D printed molds |
| title_short | A simple method to align cells on 3D hydrogels using 3D printed molds |
| title_sort | simple method to align cells on 3d hydrogels using 3d printed molds |
| topic | Vascular smooth muscle cells Cell alignment Hydrogel patterning 3D printing |
| url | http://www.sciencedirect.com/science/article/pii/S2667099221000013 |
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