Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort Study
<strong>Background: </strong>The incidence of ischemic reperfusion injury (IRI) in early phase post-transplantation and activation of toll-like receptor (TLR-2) and TLR-4 remarkably impact the outcome of a renal allograft. <strong>Objective: </strong>To investigate whether th...
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2017-03-01
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| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | http://iji.sums.ac.ir/article_39286_9155557e5753adf15f78c908488a2326.pdf |
| _version_ | 1818355991647879168 |
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| author | Morteza Hosseinzadeh Mohsen Nafar Pedram Ahmadpoor Farshid Noorbakhsh Mir Saeed Yekaninejad Mohammad Hossein Niknam Aliakbar Amirzargar |
| author_facet | Morteza Hosseinzadeh Mohsen Nafar Pedram Ahmadpoor Farshid Noorbakhsh Mir Saeed Yekaninejad Mohammad Hossein Niknam Aliakbar Amirzargar |
| author_sort | Morteza Hosseinzadeh |
| collection | DOAJ |
| description | <strong>Background: </strong>The incidence of ischemic reperfusion injury (IRI) in early phase post-transplantation and activation of toll-like receptor (TLR-2) and TLR-4 remarkably impact the outcome of a renal allograft. <strong>Objective: </strong>To investigate whether the expression of TLRs in peripheral blood mononuclear cells (PBMCs) can predict the clinical outcome of kidney allografts. <strong>Methods:</strong> We obtained blood samples from 52 renal transplant patients before transplant, and 2, 90, and 180 days post-transplantation in order to analyze the surface expressions of TLR-2 and TLR-4 on peripheral blood monocytes. The expression patterns of TLR-2 and TLR-4 were compared between patients with graft dysfunction (GD) and those with well-functioning graft (WFG). <strong>Results: </strong>Significantly different mean dynamic changes in surface expression of TLR-2, according to percentage of TLR-2<sup>+</sup> cells, between (the GD and WFG) groups existed at most time-points before and after renal transplantation (p=0.007) with the exception of day 2 post-transplantation. We observed significantly higher mean fluorescence intensities of TLR-2 and TLR-4 on CD14<sup>+ </sup>cells in the GD group compared to the WFG group. This finding was particularly observed 180 days post-transplantation (p=0.001). Based on TLR-2 and TLR-4 protein expression for each step, multiple logistic regression and ROC curve analysis revealed that an increase in CD14<sup>+</sup> TLR-2<sup>+</sup> monocytes within the 90 days post-transplantaton was associated with increased risk of GD at 180 and 365 days post-transplantation [odds ratio (OR)=1.27, p=0.005)]. <strong>Conclusion:</strong> Sequential monitoring of TLR-2 and TLR-4 expression patterns in peripheral blood monocytes appear to be prognostic and predictive biomarkers for early and late kidney allograft outcomes. |
| first_indexed | 2024-12-13T19:50:07Z |
| format | Article |
| id | doaj.art-02f877a0073a4e318b71b0e69f48736d |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-12-13T19:50:07Z |
| publishDate | 2017-03-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-02f877a0073a4e318b71b0e69f48736d2022-12-21T23:33:27ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2017-03-01141243439286Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort StudyMorteza Hosseinzadeh0Mohsen Nafar1Pedram Ahmadpoor2Farshid Noorbakhsh3Mir Saeed Yekaninejad4Mohammad Hossein Niknam5Aliakbar Amirzargar6Department of Immunology, School of Medicine, Ilam University of Medical Sciences, Ilam, IranChronic Kidney Disease Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranChronic Kidney Disease Research Center, Shahid Beheshti University of Medical Sciences,Tehran, IranDepartment of Immunology, School of Medicine, Ilam University of Medical Sciences, Ilam, IranDepartment of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Immunology, School of Medicine, Ilam University of Medical Sciences, Ilam, IranDepartment of Immunology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran<strong>Background: </strong>The incidence of ischemic reperfusion injury (IRI) in early phase post-transplantation and activation of toll-like receptor (TLR-2) and TLR-4 remarkably impact the outcome of a renal allograft. <strong>Objective: </strong>To investigate whether the expression of TLRs in peripheral blood mononuclear cells (PBMCs) can predict the clinical outcome of kidney allografts. <strong>Methods:</strong> We obtained blood samples from 52 renal transplant patients before transplant, and 2, 90, and 180 days post-transplantation in order to analyze the surface expressions of TLR-2 and TLR-4 on peripheral blood monocytes. The expression patterns of TLR-2 and TLR-4 were compared between patients with graft dysfunction (GD) and those with well-functioning graft (WFG). <strong>Results: </strong>Significantly different mean dynamic changes in surface expression of TLR-2, according to percentage of TLR-2<sup>+</sup> cells, between (the GD and WFG) groups existed at most time-points before and after renal transplantation (p=0.007) with the exception of day 2 post-transplantation. We observed significantly higher mean fluorescence intensities of TLR-2 and TLR-4 on CD14<sup>+ </sup>cells in the GD group compared to the WFG group. This finding was particularly observed 180 days post-transplantation (p=0.001). Based on TLR-2 and TLR-4 protein expression for each step, multiple logistic regression and ROC curve analysis revealed that an increase in CD14<sup>+</sup> TLR-2<sup>+</sup> monocytes within the 90 days post-transplantaton was associated with increased risk of GD at 180 and 365 days post-transplantation [odds ratio (OR)=1.27, p=0.005)]. <strong>Conclusion:</strong> Sequential monitoring of TLR-2 and TLR-4 expression patterns in peripheral blood monocytes appear to be prognostic and predictive biomarkers for early and late kidney allograft outcomes.http://iji.sums.ac.ir/article_39286_9155557e5753adf15f78c908488a2326.pdfAllograft functionKidney transplantToll-Like Receptors |
| spellingShingle | Morteza Hosseinzadeh Mohsen Nafar Pedram Ahmadpoor Farshid Noorbakhsh Mir Saeed Yekaninejad Mohammad Hossein Niknam Aliakbar Amirzargar Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort Study Iranian Journal of Immunology Allograft function Kidney transplant Toll-Like Receptors |
| title | Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort Study |
| title_full | Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort Study |
| title_fullStr | Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort Study |
| title_full_unstemmed | Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort Study |
| title_short | Increased Expression of Toll-Like Receptors 2 and 4 in Renal Transplant Recipients that Develop Allograft Dysfunction: A Cohort Study |
| title_sort | increased expression of toll like receptors 2 and 4 in renal transplant recipients that develop allograft dysfunction a cohort study |
| topic | Allograft function Kidney transplant Toll-Like Receptors |
| url | http://iji.sums.ac.ir/article_39286_9155557e5753adf15f78c908488a2326.pdf |
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