Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure
Phenotypic plasticity is the exhibition of various phenotypic traits produced by a single genotype in response to environmental changes, enabling organisms to adapt to environmental changes by maintaining growth and reproduction. Despite its significance in evolutionary studies, we still know little...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.696730/full |
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| author | Dengcheng Yang Xuyang Zheng Libo Jiang Libo Jiang Meixia Ye Meixia Ye Xiaoqing He Xiaoqing He Yi Jin Yi Jin Rongling Wu Rongling Wu Rongling Wu |
| author_facet | Dengcheng Yang Xuyang Zheng Libo Jiang Libo Jiang Meixia Ye Meixia Ye Xiaoqing He Xiaoqing He Yi Jin Yi Jin Rongling Wu Rongling Wu Rongling Wu |
| author_sort | Dengcheng Yang |
| collection | DOAJ |
| description | Phenotypic plasticity is the exhibition of various phenotypic traits produced by a single genotype in response to environmental changes, enabling organisms to adapt to environmental changes by maintaining growth and reproduction. Despite its significance in evolutionary studies, we still know little about the genetic control of phenotypic plasticity. In this study, we designed and conducted a genome-wide association study (GWAS) to reveal genetic architecture of how Staphylococcus aureus strains respond to increasing concentrations of vancomycin (0, 2, 4, and 6 μg/mL) in a time course. We implemented functional mapping, a dynamic model for genetic mapping using longitudinal data, to map specific loci that mediate the growth trajectories of abundance of vancomycin-exposed S. aureus strains. 78 significant single nucleotide polymorphisms were identified following analysis of the whole growth and development process, and seven genes might play a pivotal role in governing phenotypic plasticity to the pressure of vancomycin. These seven genes, SAOUHSC_00020 (walR), SAOUHSC_00176, SAOUHSC_00544 (sdrC), SAOUHSC_02998, SAOUHSC_00025, SAOUHSC_00169, and SAOUHSC_02023, were found to help S. aureus regulate antibiotic pressure. Our dynamic gene mapping technique provides a tool for dissecting the phenotypic plasticity mechanisms of S. aureus under vancomycin pressure, emphasizing the feasibility and potential of functional mapping in the study of bacterial phenotypic plasticity. |
| first_indexed | 2024-12-22T10:27:13Z |
| format | Article |
| id | doaj.art-02f90350849a490eb8dc405addd5eeb7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T10:27:13Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-02f90350849a490eb8dc405addd5eeb72022-12-21T18:29:26ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-09-011210.3389/fmicb.2021.696730696730Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin PressureDengcheng Yang0Xuyang Zheng1Libo Jiang2Libo Jiang3Meixia Ye4Meixia Ye5Xiaoqing He6Xiaoqing He7Yi Jin8Yi Jin9Rongling Wu10Rongling Wu11Rongling Wu12Center for Computational Biology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCenter for Computational Biology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCenter for Computational Biology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCenter for Computational Biology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCenter for Computational Biology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCenter for Computational Biology, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaDepartment of Public Health Sciences and Statistics, Center for Statistical Genetics, The Pennsylvania State University, Hershey, PA, United StatesPhenotypic plasticity is the exhibition of various phenotypic traits produced by a single genotype in response to environmental changes, enabling organisms to adapt to environmental changes by maintaining growth and reproduction. Despite its significance in evolutionary studies, we still know little about the genetic control of phenotypic plasticity. In this study, we designed and conducted a genome-wide association study (GWAS) to reveal genetic architecture of how Staphylococcus aureus strains respond to increasing concentrations of vancomycin (0, 2, 4, and 6 μg/mL) in a time course. We implemented functional mapping, a dynamic model for genetic mapping using longitudinal data, to map specific loci that mediate the growth trajectories of abundance of vancomycin-exposed S. aureus strains. 78 significant single nucleotide polymorphisms were identified following analysis of the whole growth and development process, and seven genes might play a pivotal role in governing phenotypic plasticity to the pressure of vancomycin. These seven genes, SAOUHSC_00020 (walR), SAOUHSC_00176, SAOUHSC_00544 (sdrC), SAOUHSC_02998, SAOUHSC_00025, SAOUHSC_00169, and SAOUHSC_02023, were found to help S. aureus regulate antibiotic pressure. Our dynamic gene mapping technique provides a tool for dissecting the phenotypic plasticity mechanisms of S. aureus under vancomycin pressure, emphasizing the feasibility and potential of functional mapping in the study of bacterial phenotypic plasticity.https://www.frontiersin.org/articles/10.3389/fmicb.2021.696730/fullphenotypic plasticityStaphylococcus aureusfunctional mappingvancomycingrowth curve |
| spellingShingle | Dengcheng Yang Xuyang Zheng Libo Jiang Libo Jiang Meixia Ye Meixia Ye Xiaoqing He Xiaoqing He Yi Jin Yi Jin Rongling Wu Rongling Wu Rongling Wu Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure Frontiers in Microbiology phenotypic plasticity Staphylococcus aureus functional mapping vancomycin growth curve |
| title | Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure |
| title_full | Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure |
| title_fullStr | Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure |
| title_full_unstemmed | Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure |
| title_short | Functional Mapping of Phenotypic Plasticity of Staphylococcus aureus Under Vancomycin Pressure |
| title_sort | functional mapping of phenotypic plasticity of staphylococcus aureus under vancomycin pressure |
| topic | phenotypic plasticity Staphylococcus aureus functional mapping vancomycin growth curve |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.696730/full |
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