Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage
Abstract Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cem...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2016-01-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100247&lng=en&tlng=en |
| _version_ | 1818370188257525760 |
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| author | Ana Lívia GOMES-CORNÉLIO Elisandra Márcia RODRIGUES Leticia Boldrin MESTIERI Thaís de Oliveira Rodrigues Sanzovo FALCOSKI Christiane Pienna SOARES Juliane Maria GUERREIRO-TANOMARU Carlos ROSSA JUNIOR Mário TANOMARU FILHO |
| author_facet | Ana Lívia GOMES-CORNÉLIO Elisandra Márcia RODRIGUES Leticia Boldrin MESTIERI Thaís de Oliveira Rodrigues Sanzovo FALCOSKI Christiane Pienna SOARES Juliane Maria GUERREIRO-TANOMARU Carlos ROSSA JUNIOR Mário TANOMARU FILHO |
| author_sort | Ana Lívia GOMES-CORNÉLIO |
| collection | DOAJ |
| description | Abstract Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni’s posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials. |
| first_indexed | 2024-12-13T23:35:46Z |
| format | Article |
| id | doaj.art-02fb2e6ac78d4eafae60e51346fea824 |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-13T23:35:46Z |
| publishDate | 2016-01-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-02fb2e6ac78d4eafae60e51346fea8242022-12-21T23:27:19ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072016-01-0130110.1590/1807-3107BOR-2016.vol30.0048S1806-83242016000100247Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineageAna Lívia GOMES-CORNÉLIOElisandra Márcia RODRIGUESLeticia Boldrin MESTIERIThaís de Oliveira Rodrigues Sanzovo FALCOSKIChristiane Pienna SOARESJuliane Maria GUERREIRO-TANOMARUCarlos ROSSA JUNIORMário TANOMARU FILHOAbstract Several calcium silicate-based biomaterials have been developed in recent years, in addition to Mineral Trioxide Aggregate (MTA). The aim of this study was to evaluate the cytotoxicity, genotoxicity and apoptosis/necrosis in human osteoblast cells (SAOS-2) of pure calcium silicate-based cements (CSC) and modified formulations: modified calcium silicate-based cements (CSCM) and three resin-based calcium silicate cements (CSCR1) (CSCR 2) (CSCR3). The following tests were performed after 24 hours of cement extract exposure: methyl-thiazolyl tetrazolium (MTT), apoptosis/necrosis assay and comet assay. The negative control (CT-) was performed with untreated cells, and the positive control (CT+) used hydrogen peroxide. The data for MTT and apoptosis were submitted to analysis of variance and Bonferroni’s posttest (p < 0.05), and the data for the comet assay analysis, to the Kruskal-Wallis and Dunn tests (p < 0.05). The MTT test showed no significant difference among the materials in 2 mg/mL and 10 mg/mL concentrations. CSCR3 showed lower cell viability at 10 mg/mL. Only CSC showed lower cell viability at 50 mg/mL. CSCR1, CSCR2 and CSCR3 showed a higher percentage of initial apoptosis than the control in the apoptosis test, after 24 hours exposure. The same cements showed no genotoxicity in the concentration of 2 mg/mL, with the comet assay. CSC and CSCR2 were also not genotoxic at 10 mg/mL. All experimental materials showed viability with MTT. CSC and CSCR2 presented a better response to apoptosis and genotoxicity evaluation in the 10 mg/mL concentration, and demonstrated a considerable potential for use as reparative materials.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100247&lng=en&tlng=enCytotoxicity, ImmunologicMutagenicity TestsCalcium Compounds |
| spellingShingle | Ana Lívia GOMES-CORNÉLIO Elisandra Márcia RODRIGUES Leticia Boldrin MESTIERI Thaís de Oliveira Rodrigues Sanzovo FALCOSKI Christiane Pienna SOARES Juliane Maria GUERREIRO-TANOMARU Carlos ROSSA JUNIOR Mário TANOMARU FILHO Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage Brazilian Oral Research Cytotoxicity, Immunologic Mutagenicity Tests Calcium Compounds |
| title | Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
| title_full | Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
| title_fullStr | Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
| title_full_unstemmed | Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
| title_short | Cytotoxicity and genotoxicity of calcium silicate-based cements on an osteoblast lineage |
| title_sort | cytotoxicity and genotoxicity of calcium silicate based cements on an osteoblast lineage |
| topic | Cytotoxicity, Immunologic Mutagenicity Tests Calcium Compounds |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242016000100247&lng=en&tlng=en |
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