Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)

Evaluation of parameters affecting switchgrass tissue culture: toward a consolidated procedure for Agrobacterium-mediated transformation of switchgrass (Panicum virgatum)

Abstract Background Switchgrass (Panicum virgatum), a robust perennial C4-type grass, has been evaluated and designated as a model bioenergy crop by the U.S. DOE and USDA. Conventional breeding of switchgrass biomass is difficult because it displays self-incompatible hindrance. Therefore, direct gen...

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Bibliographic Details
Main Authors: Chien-Yuan Lin, Bryon S. Donohoe, Neha Ahuja, Deborah M. Garrity, Rongda Qu, Melvin P. Tucker, Michael E. Himmel, Hui Wei
Format: Article
Language:English
Published: BMC 2017-12-01
Series:Plant Methods
Subjects:
Switchgrass
Bioenergy crop
Red fluorescent protein (RFP)
β-Glucuronidase (GUS) staining
Agrobacterium-mediated transformation
Online Access:http://link.springer.com/article/10.1186/s13007-017-0263-6

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http://link.springer.com/article/10.1186/s13007-017-0263-6

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