Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model
Nanosized delivery systems have been the subject of research and discussion in the scientific community due to their unique properties and functionality. However, studies reporting the behaviour of nanodelivery systems under dynamic <i>in vitro</i> digestion conditions are still very sca...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/13/15/2237 |
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| author | Daniel A. Madalena João F. Araújo Óscar L. Ramos António A. Vicente Ana C. Pinheiro |
| author_facet | Daniel A. Madalena João F. Araújo Óscar L. Ramos António A. Vicente Ana C. Pinheiro |
| author_sort | Daniel A. Madalena |
| collection | DOAJ |
| description | Nanosized delivery systems have been the subject of research and discussion in the scientific community due to their unique properties and functionality. However, studies reporting the behaviour of nanodelivery systems under dynamic <i>in vitro</i> digestion conditions are still very scarce. To address this gap, this study aims to assess the dynamic <i>in vitro</i> gastric digestion of lactoferrin/curcumin nanoparticles in the realistic gastric model (RGM). For this purpose, the INFOGEST standard semi-dynamic digestion protocol was used. The nanosystems were characterized in terms of hydrodynamic size, size distribution, polydispersity index (PdI), and zeta potential using dynamic light scattering (DLS), before and during the digestion process. Confocal laser scanning microscopy (CLSM) was also used to examine particle aggregation. In addition, the release of curcumin was evaluated spectroscopically and the intrinsic fluorescence of lactoferrin was measured throughout the digestion process. The protein hydrolysis was also determined by UV-VIS-SWNIR spectroscopy to estimate, in real-time, the presence of free NH2 groups during gastric digestion. It was possible to observe that lactoferrin/curcumin nanoparticles were destabilized during the dynamic digestion process. It was also possible to conclude that low sample volumes can pose a major challenge in the application of dynamic <i>in vitro</i> digestion models. |
| first_indexed | 2024-03-11T00:20:07Z |
| format | Article |
| id | doaj.art-02ffe1b1ee654326a29d517a90ba73fa |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T00:20:07Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-02ffe1b1ee654326a29d517a90ba73fa2023-11-18T23:21:59ZengMDPI AGNanomaterials2079-49912023-08-011315223710.3390/nano13152237Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric ModelDaniel A. Madalena0João F. Araújo1Óscar L. Ramos2António A. Vicente3Ana C. Pinheiro4CEB—Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalCEB—Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalCBQF—Centro de Biotecnologia e Química Fina, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, 4202-401 Porto, PortugalCEB—Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalCEB—Centre of Biological Engineering, University of Minho, 4710-057 Braga, PortugalNanosized delivery systems have been the subject of research and discussion in the scientific community due to their unique properties and functionality. However, studies reporting the behaviour of nanodelivery systems under dynamic <i>in vitro</i> digestion conditions are still very scarce. To address this gap, this study aims to assess the dynamic <i>in vitro</i> gastric digestion of lactoferrin/curcumin nanoparticles in the realistic gastric model (RGM). For this purpose, the INFOGEST standard semi-dynamic digestion protocol was used. The nanosystems were characterized in terms of hydrodynamic size, size distribution, polydispersity index (PdI), and zeta potential using dynamic light scattering (DLS), before and during the digestion process. Confocal laser scanning microscopy (CLSM) was also used to examine particle aggregation. In addition, the release of curcumin was evaluated spectroscopically and the intrinsic fluorescence of lactoferrin was measured throughout the digestion process. The protein hydrolysis was also determined by UV-VIS-SWNIR spectroscopy to estimate, in real-time, the presence of free NH2 groups during gastric digestion. It was possible to observe that lactoferrin/curcumin nanoparticles were destabilized during the dynamic digestion process. It was also possible to conclude that low sample volumes can pose a major challenge in the application of dynamic <i>in vitro</i> digestion models.https://www.mdpi.com/2079-4991/13/15/2237<i>in vitro</i> digestiondynamic gastric digestionnanodelivery systemnanostructuresbioaccessibility |
| spellingShingle | Daniel A. Madalena João F. Araújo Óscar L. Ramos António A. Vicente Ana C. Pinheiro Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model Nanomaterials <i>in vitro</i> digestion dynamic gastric digestion nanodelivery system nanostructures bioaccessibility |
| title | Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model |
| title_full | Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model |
| title_fullStr | Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model |
| title_full_unstemmed | Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model |
| title_short | Assessing the <i>In Vitro</i> Digestion of Lactoferrin-Curcumin Nanoparticles Using the Realistic Gastric Model |
| title_sort | assessing the i in vitro i digestion of lactoferrin curcumin nanoparticles using the realistic gastric model |
| topic | <i>in vitro</i> digestion dynamic gastric digestion nanodelivery system nanostructures bioaccessibility |
| url | https://www.mdpi.com/2079-4991/13/15/2237 |
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