Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast Brazil
<i>Staphylococcus aureus</i> is a notorious human pathogen associated with serious nosocomial and community-acquired infections, such as pneumonia, meningitis, endocarditis, toxic shock syndrome, and sepsis, among others. The objective of this study was to investigate the molecular profi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/7/210 |
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| author | Andrea de S. Monteiro Bruna L. S. Pinto Joveliane de M. Monteiro Rômulo M. Ferreira Patrícia C. S. Ribeiro Silvia Y. Bando Sirlei G. Marques Luís C. N. Silva Wallace R. Nunes Neto Gabriella F. Ferreira Maria Rosa Q. Bomfim Afonso G. Abreu |
| author_facet | Andrea de S. Monteiro Bruna L. S. Pinto Joveliane de M. Monteiro Rômulo M. Ferreira Patrícia C. S. Ribeiro Silvia Y. Bando Sirlei G. Marques Luís C. N. Silva Wallace R. Nunes Neto Gabriella F. Ferreira Maria Rosa Q. Bomfim Afonso G. Abreu |
| author_sort | Andrea de S. Monteiro |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> is a notorious human pathogen associated with serious nosocomial and community-acquired infections, such as pneumonia, meningitis, endocarditis, toxic shock syndrome, and sepsis, among others. The objective of this study was to investigate the molecular profile, antimicrobial resistance, and clonal diversity of <i>S. aureus</i> isolated from the bloodstream. The determination of the minimum inhibitory concentration (MIC) of the antimicrobial was performed by an automated method. The presence of several virulence and resistance genes was evaluated by PCR. In addition, multilocus sequence typing (MLST) was used to analyze the clonal diversity of <i>S. aureus</i>. A high resistance to oxacillin (78%), clindamycin (78%), erythromycin (70%), ciprofloxacin (61%), and gentamicin (52%) was observed among the isolates. In most of them, the following virulence genes were detected: <i>hlb</i> (83%), <i>ebpS</i> (61%), <i>icaA</i> (57%), <i>fnbpA</i> (17%), and <i>clfA</i> (13%). Only one isolate carried the <i>pvl</i> gene. MLST analysis identified five new sequence types (STs): 5429, 5430, 5431, 5432, and 5433, as well as another seven—ST5, ST97, ST398, ST101, ST30, ST461, and ST2779—among the remaining strains. These seven STs and the four new STs are clustered in four clonal complexes: CC1, CC2, CC7, and CC17. Phylogenetic analysis showed the genetic relationship of the five new ST strains with another 18 strains. Altogether, these analyses indicate the horizontal transfer acquisition of virulence factor genes and multidrug resistance. |
| first_indexed | 2024-12-20T04:26:31Z |
| format | Article |
| id | doaj.art-0300570900f440899b13d2c3d544db72 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-20T04:26:31Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-0300570900f440899b13d2c3d544db722022-12-21T19:53:29ZengMDPI AGMicroorganisms2076-26072019-07-017721010.3390/microorganisms7070210microorganisms7070210Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast BrazilAndrea de S. Monteiro0Bruna L. S. Pinto1Joveliane de M. Monteiro2Rômulo M. Ferreira3Patrícia C. S. Ribeiro4Silvia Y. Bando5Sirlei G. Marques6Luís C. N. Silva7Wallace R. Nunes Neto8Gabriella F. Ferreira9Maria Rosa Q. Bomfim10Afonso G. Abreu11Programa de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, BrazilPrograma de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, BrazilPrograma de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, BrazilPrograma de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, BrazilLaboratório Cedro, São Luís 65020-570, BrazilDepartamento de Pediatria, Faculdade de Medicina da Universidade de São Paulo (FMUSP), São Paulo 01246-903, BrazilLaboratório Cedro, São Luís 65020-570, BrazilPrograma de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, BrazilPrograma de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, BrazilDepartamento de Farmácia, Universidade Federal de Juiz de Fora, Governador Valadares 35010-177, BrazilPrograma de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, BrazilPrograma de Pós-Graduação, Universidade Ceuma, São Luís 65075-120, Brazil<i>Staphylococcus aureus</i> is a notorious human pathogen associated with serious nosocomial and community-acquired infections, such as pneumonia, meningitis, endocarditis, toxic shock syndrome, and sepsis, among others. The objective of this study was to investigate the molecular profile, antimicrobial resistance, and clonal diversity of <i>S. aureus</i> isolated from the bloodstream. The determination of the minimum inhibitory concentration (MIC) of the antimicrobial was performed by an automated method. The presence of several virulence and resistance genes was evaluated by PCR. In addition, multilocus sequence typing (MLST) was used to analyze the clonal diversity of <i>S. aureus</i>. A high resistance to oxacillin (78%), clindamycin (78%), erythromycin (70%), ciprofloxacin (61%), and gentamicin (52%) was observed among the isolates. In most of them, the following virulence genes were detected: <i>hlb</i> (83%), <i>ebpS</i> (61%), <i>icaA</i> (57%), <i>fnbpA</i> (17%), and <i>clfA</i> (13%). Only one isolate carried the <i>pvl</i> gene. MLST analysis identified five new sequence types (STs): 5429, 5430, 5431, 5432, and 5433, as well as another seven—ST5, ST97, ST398, ST101, ST30, ST461, and ST2779—among the remaining strains. These seven STs and the four new STs are clustered in four clonal complexes: CC1, CC2, CC7, and CC17. Phylogenetic analysis showed the genetic relationship of the five new ST strains with another 18 strains. Altogether, these analyses indicate the horizontal transfer acquisition of virulence factor genes and multidrug resistance.https://www.mdpi.com/2076-2607/7/7/210<i>Staphylococcus aureus</i>bloodstream infectionmultilocus sequence typing (MLST)antimicrobial resistance |
| spellingShingle | Andrea de S. Monteiro Bruna L. S. Pinto Joveliane de M. Monteiro Rômulo M. Ferreira Patrícia C. S. Ribeiro Silvia Y. Bando Sirlei G. Marques Luís C. N. Silva Wallace R. Nunes Neto Gabriella F. Ferreira Maria Rosa Q. Bomfim Afonso G. Abreu Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast Brazil Microorganisms <i>Staphylococcus aureus</i> bloodstream infection multilocus sequence typing (MLST) antimicrobial resistance |
| title | Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast Brazil |
| title_full | Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast Brazil |
| title_fullStr | Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast Brazil |
| title_full_unstemmed | Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast Brazil |
| title_short | Phylogenetic and Molecular Profile of <i>Staphylococcus aureus</i> Isolated from Bloodstream Infections in Northeast Brazil |
| title_sort | phylogenetic and molecular profile of i staphylococcus aureus i isolated from bloodstream infections in northeast brazil |
| topic | <i>Staphylococcus aureus</i> bloodstream infection multilocus sequence typing (MLST) antimicrobial resistance |
| url | https://www.mdpi.com/2076-2607/7/7/210 |
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