Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice
Echinochrome A (Ech A, 7-ethyl-2,3,5,6,8-pentahydroxy-1,4-naphthoquinone) has been known to exhibit anti-oxidative and anti-inflammatory effects. However, no study has been carried out on the efficacy of Ech A against skin photoaging; this process is largely mediated by oxidative stress. Six-week-ol...
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MDPI AG
2021-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/10/550 |
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| author | Jung Eun Seol Sang Woo Ahn Bomin Seol Hyeong Rok Yun Nammi Park Hyoung Kyu Kim Elena A. Vasileva Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Jin Han |
| author_facet | Jung Eun Seol Sang Woo Ahn Bomin Seol Hyeong Rok Yun Nammi Park Hyoung Kyu Kim Elena A. Vasileva Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Jin Han |
| author_sort | Jung Eun Seol |
| collection | DOAJ |
| description | Echinochrome A (Ech A, 7-ethyl-2,3,5,6,8-pentahydroxy-1,4-naphthoquinone) has been known to exhibit anti-oxidative and anti-inflammatory effects. However, no study has been carried out on the efficacy of Ech A against skin photoaging; this process is largely mediated by oxidative stress. Six-week-old male SKH-1 hairless mice (<i>n</i> = 36) were divided into five groups. Except for a group that were not treated (<i>n</i> = 4), all mice underwent ultraviolet-B (UVB) exposure for 8 weeks while applying phosphate-buffered saline or Ech A through intraperitoneal injection. UVB impaired skin barrier function, showing increased transepidermal water loss and decreased stratum corneum hydration. UVB induced dermal collagen degeneration and mast cell infiltration. Ech A injection was found to significantly lower transepidermal water loss while attenuating tissue inflammatory changes and collagen degeneration compared to the control. Furthermore, Ech A was found to decrease the relative expression of matrix metalloproteinase, tryptase, and chymase. Taken together, these results suggest that Ech A protects against UVB-induced photoaging in both functional and histologic aspects, causing a lowering of collagen degradation and inflammatory cell infiltration. |
| first_indexed | 2024-03-10T06:25:33Z |
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| id | doaj.art-0300de4d78cd4e1cbe1104da74dd0e16 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T06:25:33Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-0300de4d78cd4e1cbe1104da74dd0e162023-11-22T18:55:11ZengMDPI AGMarine Drugs1660-33972021-09-01191055010.3390/md19100550Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless MiceJung Eun Seol0Sang Woo Ahn1Bomin Seol2Hyeong Rok Yun3Nammi Park4Hyoung Kyu Kim5Elena A. Vasileva6Natalia P. Mishchenko7Sergey A. Fedoreyev8Valentin A. Stonik9Jin Han10Department of Dermatology, Inje University Busan Paik Hospital, College of Medicine, Inje University, Busan 47392, KoreaDepartment of Dermatology, Inje University Busan Paik Hospital, College of Medicine, Inje University, Busan 47392, KoreaDepartment of Dermatology, Inje University Busan Paik Hospital, College of Medicine, Inje University, Busan 47392, KoreaDepartment of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Smart Marine Therapeutic Center, Busan 47392, KoreaDepartment of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Smart Marine Therapeutic Center, Busan 47392, KoreaDepartment of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Smart Marine Therapeutic Center, Busan 47392, KoreaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 690022 Vladivostok, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 690022 Vladivostok, RussiaDepartment of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center, Smart Marine Therapeutic Center, Busan 47392, KoreaEchinochrome A (Ech A, 7-ethyl-2,3,5,6,8-pentahydroxy-1,4-naphthoquinone) has been known to exhibit anti-oxidative and anti-inflammatory effects. However, no study has been carried out on the efficacy of Ech A against skin photoaging; this process is largely mediated by oxidative stress. Six-week-old male SKH-1 hairless mice (<i>n</i> = 36) were divided into five groups. Except for a group that were not treated (<i>n</i> = 4), all mice underwent ultraviolet-B (UVB) exposure for 8 weeks while applying phosphate-buffered saline or Ech A through intraperitoneal injection. UVB impaired skin barrier function, showing increased transepidermal water loss and decreased stratum corneum hydration. UVB induced dermal collagen degeneration and mast cell infiltration. Ech A injection was found to significantly lower transepidermal water loss while attenuating tissue inflammatory changes and collagen degeneration compared to the control. Furthermore, Ech A was found to decrease the relative expression of matrix metalloproteinase, tryptase, and chymase. Taken together, these results suggest that Ech A protects against UVB-induced photoaging in both functional and histologic aspects, causing a lowering of collagen degradation and inflammatory cell infiltration.https://www.mdpi.com/1660-3397/19/10/550echinochrome Aphotoaginghairless micedermal collagen degenerationmast cell infiltration |
| spellingShingle | Jung Eun Seol Sang Woo Ahn Bomin Seol Hyeong Rok Yun Nammi Park Hyoung Kyu Kim Elena A. Vasileva Natalia P. Mishchenko Sergey A. Fedoreyev Valentin A. Stonik Jin Han Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice Marine Drugs echinochrome A photoaging hairless mice dermal collagen degeneration mast cell infiltration |
| title | Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice |
| title_full | Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice |
| title_fullStr | Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice |
| title_full_unstemmed | Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice |
| title_short | Echinochrome A Protects against Ultraviolet B-induced Photoaging by Lowering Collagen Degradation and Inflammatory Cell Infiltration in Hairless Mice |
| title_sort | echinochrome a protects against ultraviolet b induced photoaging by lowering collagen degradation and inflammatory cell infiltration in hairless mice |
| topic | echinochrome A photoaging hairless mice dermal collagen degeneration mast cell infiltration |
| url | https://www.mdpi.com/1660-3397/19/10/550 |
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